Bench PartnersBench Partners Video SeriesChromatography VideoTips and Techniques VideoVideos

Removing Air Bubbles from the NGC™ Chromatography System

This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles.
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Bench PartnersBench Partners Video SeriesChromatography VideoTips and Techniques VideoVideos

Using the Air Sensor on the NGC™ Chromatography System

This video describes the functions of the Air Sensor Module and how to connect it to the NGC Chromatography System. The NGC Air Sensor senses the end of sample or buffer and prevents the introduction of air into the system, which can damage columns and/or incur the loss of precious samples. Both small and large air sensors are available to fit the tubing being used. Steps for connecting the Air Sensor Module and associated air sensors are described including how to add the air sensors into the system flow path using ChromLab™ Software and the differences in using the air sensors in manual vs. method modes.
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ArticlesChromatographyProduct Highlights

Purifying Difficult-to-Purify Proteins

Protein purification becomes a challenging step for applications requiring high protein concentrations (such as structural biology studies). This is also true for researchers purifying integral membranes proteins and untagged proteins from native sources. Here we provide some useful tips for selecting the proper resins, optimizing conditions and developing effective strategies to tackle the challenges associated with such difficult-to-purify proteins.
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ArticlesChromatographyChromatography VideoProduct HighlightsVideos

How Do You Automate a Multicolumn Protein Purification?

Multicolumn purifications are tedious and the manual operations during this process introduce variabilities, affecting the success of purification and reproducibility of results. This whiteboard animation explains how an automated multi-dimensional chromatography method can make this an easier process with very high success rates.
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Past WebinarsWebinars

Overcoming Purification Hurdles with Mixed-Mode Chromatography

Dr. Mark Snyder, a leading expert in the field of protein purification, presents the purification process development for two very different targets, adenovirus and bacterial surface proteins. In both cases, a two-step column process results in a preparation with high yield and activity and very low host cell protein and DNA contamination. Conditions used make the methods suitable for large-scale production. In this webinar you will learn about: Advantages of mixed-mode resins Optimizing purification process development Procedures for purifying adenovirus and bacterial surface proteins  
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Past WebinarsWebinars

Characterizing Functional Domains of Potential Therapeutics with Better Protein Purification

Dr. John Bruning (University of Adelaide) and Dr. Jiansheng Wu (Genentech), two leading experts in the field of protein purification, share exciting examples of their research and recent chromatography tools they have used to demonstrate robust and improved efficiencies to their discovery and characterization protein purification workflows.
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ArticlesChromatographyTechnical Reports

Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes

The separation of monomers from product-related impurities is quite challenging in process separations. It requires a thorough screening and an efficient resin to get efficiency in the purification process. Review the performance data of Bio-Rad’s mixed mode CHT™ Ceramic Hydroxapatite in achieving process separation efficiency and how it compares with various Capto media.
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ArticlesChromatographyChromatography VideoVideos

How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution?

The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.
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ArticlesChromatographyProtocols and Tips

Chromatography: Everything You Need to Know about Your Peaks

What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.
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Tip of the Week

#7 How Do You Detect DNA Contamination in Your Protein During Purification?

Here are some ways you can detect DNA contamination during the protein purification process.
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