ArticlesChromatographyChromatography VideoVideos

How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution?

The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.
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ArticlesChromatographyProtocols and Tips

Chromatography: Everything You Need to Know about Your Peaks

What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.
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Tip of the Week

#7 How Do You Detect DNA Contamination in Your Protein During Purification?

Here are some ways you can detect DNA contamination during the protein purification process.
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Tip of the Week

#5 How Do You Choose the Right Hardware for Your Chromatography Application?

Which valves, pumps or detectors are optimal for your FPLC system? How do you achieve optimal gradient performance? Here are some tips.
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Optimizing to Automation: The Advantages of Multidimensional (Multi-D) Chromatography

Philip Chapman of Bio-Rad Laboratories discusses the advantages of automated multidimensional (Multi-D) chromatography compared to traditional multistep purification strategies.  
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Bench PartnersChromatography VideoLab NotesVideos

Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography

From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don’t have to worry about proteases, we share helpful tips for developing a sample preparation protocol.
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Bench PartnersChromatography VideoLab NotesVideos

Five Tips for Picking the Right Cell Disruption Method for Protein Analysis

Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.
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Tip of the Week

#4 How Do You Listen to Your FPLC UV Detector?

Here are some tips to fix random spikes in your peaks and pulsations in your gradient.
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ArticlesChromatographyProduct Highlights

Bio-Rad’s Multi-D Method Addresses Unmet Chromatography Needs

Some of the frustrations in protein purification stem from unmet chromatography needs, such as automation, productivity, throughput, and reproducibility among others. Bio-Rad’s new Multi-D chromatography method addresses these and provides solutions to faster, efficient and more reliable protein purification.
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