Which valves, pumps or detectors are optimal for your FPLC system? How do you achieve optimal gradient performance? Here are some tips.
Philip Chapman of Bio-Rad Laboratories discusses the advantages of automated multidimensional (Multi-D) chromatography compared to traditional multistep purification strategies.
From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don’t have to worry about proteases, we share helpful tips for developing a sample preparation protocol.
Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.
Some of the frustrations in protein purification stem from unmet chromatography needs, such as automation, productivity, throughput, and reproducibility among others. Bio-Rad’s new Multi-D chromatography method addresses these and provides solutions to faster, efficient and more reliable protein purification.
Here are some handy tips for maintaining your chromatography columns in great shape.
Here are some tips for attaching, filling or running your chromatography columns.
Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography
Traditional sequential chromatography typically requires intensive hands-on user manipulation, which could hinder the reproducibility of protein purification runs. Multi-D chromatography aims to improve this process while maintaining the efficacy of the traditional workflow.
Here are some tips to clarify your lysate, filter proteins, and to achieve optimal binding of your proteins.