chromatography

Traditional sequential chromatography typically requires intensive hands-on user manipulation, which could hinder the reproducibility of protein purification runs. Multi-D chromatography aims to improve this process while maintaining the efficacy of the traditional workflow.

Here are some tips to clarify your lysate, filter proteins, and to achieve optimal binding of your proteins.

Doing these three simple checks will protect your chromatography system from common lab hazards.

Here are the methods you need to ensure optimal, long-lasting column performance.

Follow these steps to go from crude cell lysate to a sample that’s ready to purify.

Bioradiations, Bio-Rad’s technical resource for life science research, sent out its first issue in 1965. How did this publication evolve in the years that followed? Take a look in our archives and find out!

The NGC Chromatography System introduces a new level of modularity and usability for protein purification. The modular design of the NGC System provides multiple configurations, each addressing different requirements. Use our configurator tool to build your own system to meet your specific workflow and throughput needs.

See how researcher Petr Novák, a structural biologist in Prague, Czech Republic, has accelerated his effort to characterize complex receptor molecules by integrating the NGC chromatography system into his lab’s workflow.

What does automated protein purification mean today? What might it look like tomorrow? Bioradiations talks to a variety of users about what they’re doing now to implement automation in their purification processes, and what possibilities they envision that will improve chromatography in the near future.

While using affinity tagging is the preferred method to achieve highly pure protein through recombinant expression, this approach isn’t feasible for all proteins. This tech report describes workflow optimizations using the NGC Chromatography System that allow users to achieve high purities with untagged proteins.