Electrophoresis/Western Blotting

For years, CCD sensors, known for their high-quality imaging capabilities, have been the gold standard in scientific instrumentation. However, recent advancements in CMOS sensors have positioned them as a cost-effective and competitive alternative that is quickly gaining traction in the field. In this article, we delve into the strengths and applications of CCD and CMOS sensors, exploring how this shift in technology is reshaping the digital imaging landscape.

Maintaining compliance while leveraging advanced digital imaging technologies can be a daunting task. Luckily, Bio-Rad Image Lab Touch Software takes this challenge head-on by offering a seamless solution for digital imaging. Learn how Image Lab Touch Software integrates with Bio-Rad imaging systems to support researchers in meeting stringent regulatory requirements, ensuring data integrity and security without compromising efficiency or ease of use.

A groundbreaking addition to imaging technology has arrived — the ChemiDoc Go Imaging System. The ChemiDoc Go Imaging System is tailored for both gel and western blot imaging, including chemiluminescence and StarBright Blue fluorescent detection. Its compatibility with various trays and LED configurations ensures optimal performance across different experimental setups, including gel excision, nucleic acid staining, and protein detection.

Protein expression analysis is essential in cell biology research, as it provides valuable insights into the molecular mechanisms that underlie cellular functions. Traditional methods for protein analysis, such as western blotting and ELISA, are useful for studying protein expression in cell lysates. However, these methods require labor-intensive sample preparation and do not provide information on the spatial distribution of proteins within intact cells. In-Cell Western is a powerful technique that enables quantitative and qualitative in situ protein expression analysis.

Do you think you know western blotting? Test your knowledge with a crossword puzzle.

Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.

The next iteration of Bio-Rad Gel Documentation Systems has arrived. The GelDoc Go Imaging System gives you a benchtop imaging solution that enables gel documentation in three simple steps. Acquire high-resolution, publication-quality images of nucleic acid gels, protein gels, and colorimetric blots. Image up to four mini gels at once with the large imaging area, and easily set up and control the GelDoc Go Imaging System with Image Lab Touch Software. Read more about the compact, easy-to-use GelDoc Go System.

Western blotting is a well-accepted technique which has a place in every molecular biologist’s toolkit. However, the traditional process is lengthy and filled with manual steps, which can result in variability. Stain-Free technology can shorten your workflow and improve your data. Read our top three reasons to incorporate Stain-Free technology in your western blotting workflow.

Join us for an in-depth dive into western blotting. Whether you are new to western blotting or an experienced user looking for tips, this five-part webinar series has something for you. Slides and downloadable resources will be available for each webinar. Complete all five classes to receive a certificate of completion and a colorful Western Blotting University poster.

Presented by: Gary F. Ross, PhD, Proteomics Field Application Scientist

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Learn best practices for fluorescent western blotting, including multiplex analysis and image acquisition.