Bench PartnersBench Partners Video SeriesElectrophoresis/Western Blotting VideoTips and Techniques VideoVideos

TGX Stain-Free™ FastCast™ Acrylamide Gel Casting

This short video demonstrates how you can access the unique benefits of Bio-Rad’s proprietary TGX Stain-Free Gel chemistry by hand casting your own TGX Stain-Free Gels in just a few quick and easy steps.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Six Tips for Efficient Transfer of Your Proteins

A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.
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ChromatographyProtocols and Tips

Tips and Strategies for Successful Purification of Your Proteins

A biomolecule protein purification workflow requires more than one resin. The trick is to find the combination of resins that provides the highest purity in the least number of steps and at the lowest cost. Here are some guides to select the optimal resins for the purification of monoclonal antibodies, native and recombinant proteins, Ig G and mammalian and adenovirus, and for removing aggregates and endotoxins.
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ArticlesElectrophoresis/Western BlottingFeatured StoriesProduct HighlightsProtocols and TipsReal-time qPCR/PCR

A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates

It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Tips for Selecting the Best Gel for Your Application

Picking the right gel for your application is not easy. It depends on so many factors, such as the purpose of your application, the size of your protein, what kind of buffer you need to have in your sample etc. Here we provide a guide for you to choose the best Bio-Rad gel based on all these factors.
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ArticlesBio-Plex Multiplex AssaysProtocols and Tips

8 Tips for Understanding Multiplex Immunoassay Development and Validation

Multiplex immunoassay development requires a lot of validation and standardization steps. Several factors can influence the performance of these assays. Some factors that need to be considered are mentioned here.
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ArticlesBio-Plex Multiplex AssaysProtocols and Tips

List of Mouse Lineage Biomarkers for Different Immune Cell Types

How can we easily identify biomarkers for different immune cell types? This color-coded poster makes identification of mouse lineage biomarkers easy.
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ArticlesChromatographyProtocols and Tips

Chromatography: Everything You Need to Know about Your Peaks

What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.
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ArticlesCell BiologyProtocols and Tips

Flow Cytometry: Basic Definitions

It’s always good to get the basics straight. Here, we’ve defined some flow cytometry terms that might have eluded you.
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ArticlesCell BiologyProtocols and Tips

10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels

Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.
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