Chromatography VideoVideos

A Mixed-Mode Approach — An Alternative to a Three-Step mAb Purification Process

Monoclonal antibody (mAb) purification methods often involve the use of resin-based chromatography. In this video, we compare a three-step mAb purification workflow, which uses Nuvia Ion Exchange Resins post–Protein A capture, to a two-step purification workflow, which includes a Protein A capture step followed by a mixed-mode chromatography step using Nuvia aPrime 4A Resin, a hydrophobic anion exchange resin. We present an additional two-step purification approach using Protein A resin followed by a calcium affinity cation exchange media, CHT Ceramic Hydroxyapatite XT Media. The results of both studies demonstrate that a high purity product can be attained using one fewer unit operation than traditional workflows.
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Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples
ArticlesFeatured StoriesProtocols and TipsReal-time qPCR/PCR

Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples

Incorporating preamplification into your qPCR experiments may seem daunting, but it is a powerful way to analyze targets from limited samples. The tips in this article will help you understand how preamplification can enhance your qPCR experiments and the considerations and implications for incorporating it into your workflow.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Tips to Make Fluorophore Picking Easier

Fluorescence is a property of photo-excitable molecules that has been exploited to develop dyes, or fluorophores, that are used in a variety of experimental procedures, including western blotting. Learn more about the science behind fluorescent dyes and get some helpful tips on how to pick the right fluorophores for your experimental needs.
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ArticlesFeatured StoriesProtocols and Tips

Key Steps to Ensure Optimized and Reproducible qPCR Experiments

Real-time PCR is a common and easy-to-adopt technology. By keeping a few simple steps in mind as you design and run your experiments you can ensure high-quality, reproducible data.
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ArticlesDrug Discovery and DevelopmentProtocols and Tips

Characterization of Critical Reagents for Ligand Binding Assays

Ligand binding assays are utilized throughout the large-molecule drug development process. However, their robustness, accuracy, and reproducibility depend on the quality of critical reagents. See how you can ensure their consistent characterization, particularly during early drug development stages.
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ArticlesBio-Plex Multiplex AssaysProtocols and Tips

Multiplex Immunoassays: Five Tips for Optimizing Your Experiments

Follow the five tips in this article before embarking on your multiplex immunoassay journey to generate accurate, efficient, and biologically relevant results. From help designing experiments to acquiring accurate data, this article has it all.
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ArticlesDroplet Digital PCR (ddPCR)Featured StoriesProtocols and Tips

Digital PCR: Helpful Tips When Using Droplet Partitioning Technology

Digital PCR offers a highly sensitive alternative to conventional qPCR, enabling absolute quantification of nucleic acids and rare allele detection. The tips in this article help you set up and optimize your digital PCR experiments when using droplet partitioning technology.
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Bench PartnersBench Partners Video SeriesElectrophoresis/Western Blotting VideoTips and Techniques VideoVideos

TGX Stain-Free™ FastCast™ Acrylamide Gel Casting

This short video demonstrates how you can access the unique benefits of Bio-Rad’s proprietary TGX Stain-Free Gel chemistry by hand casting your own TGX Stain-Free Gels in just a few quick and easy steps.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Six Tips for Efficient Transfer of Your Proteins

A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.
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ChromatographyProtocols and Tips

Tips and Strategies for Successful Purification of Your Proteins

A biomolecule protein purification workflow requires more than one resin. The trick is to find the combination of resins that provides the highest purity in the least number of steps and at the lowest cost. Here are some guides to select the optimal resins for the purification of monoclonal antibodies, native and recombinant proteins, Ig G and mammalian and adenovirus, and for removing aggregates and endotoxins.
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