ArticlesElectrophoresis/Western BlottingGeneral Interest

Defining the New Normal in Quantitative Western Blot Data

Normalization of western blot data is very crucial in quantitating proteins. Normalization of band intensity of proteins of interest with the band intensity of housekeeping proteins (HKP) is routinely done in labs. But more and more journals question the validity of using HKPs in normalization and are demanding more validations and tests. An alternative method is to use total protein normalization (TPN) for normalization. Explore the TPN option and see how you can get reliable western blot data easily.
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Electrophoresis/Western BlottingPast WebinarsWebinars

Generating Semi-Quantitative Western Blot Data Using Bio-Rad’s Image Lab™ 6.0 Software

Presented by: Kenneth J. Oh, PhD, Applications, Collaborations, and New Technology Manager, Protein Quantitation Marketing Group, Bio-Rad Laboratories, Inc.
Date: Tuesday, March 6, 2018

Western blotting is a popular tool for protein quantitation, and the advent of digital imaging has greatly increased the sensitivity, dynamic range, and quantitation limits of this technique. In the second webinar in this series, get step-to-step instructions for normalizing western blot data using Image Lab 6.0 Software.

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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Tips to Make Fluorophore Picking Easier

Fluorescence is a property of photo-excitable molecules that has been exploited to develop dyes, or fluorophores, that are used in a variety of experimental procedures, including western blotting. Learn more about the science behind fluorescent dyes and get some helpful tips on how to pick the right fluorophores for your experimental needs.
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ArticlesElectrophoresis/Western BlottingGeneral Interest

Identify Major Immune Cell Types by Their CD Marker Expression

Our new immune cell lineage and marker database provides an interactive way to help you easily examine detailed marker data, view immune cell marker expression, and find the antibody to your marker. Learn more and try out the interactive tool for yourself.
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ArticlesElectrophoresis/Western BlottingGeneral Interest

Validating Antibodies — the Good, the Bad, and the Necessary

Despite the fact that antibodies are one of the most frequently used tools in life science and clinical research, there are no universally accepted guidelines to ensure that they have been properly validated. Learn about the issues facing researchers and what antibody suppliers and researchers can do to ensure that the research being carried out is trustworthy and is helping to improve overall data quality.
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ArticlesElectrophoresis/Western BlottingFeatured Stories

Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol

Western blotting using fluorescently tagged detection antibodies is a technique that is gaining in popularity. However, adopting a new technique can be challenging. Use the tips provided in this article to get started and see how fluorescent western blotting can enhance your scientific research.
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ArticlesElectrophoresis/Western BlottingFeatured StoriesGeneral Interest

The Fluorescent Way: Why Start Fluorescent Western Blotting Now?

Find out why fluorescent western blotting is gaining in popularity and how it compares to more widely used methods, such as chemiluminescence. From easier multiplexing to brighter fluorophores to easier method development, learn about the many advantages of fluorescent western blotting.
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Bench PartnersBench Partners Video SeriesElectrophoresis/Western Blotting VideoTips and Techniques VideoVideos

TGX Stain-Free™ FastCast™ Acrylamide Gel Casting

This short video demonstrates how you can access the unique benefits of Bio-Rad’s proprietary TGX Stain-Free Gel chemistry by hand casting your own TGX Stain-Free Gels in just a few quick and easy steps.
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Electrophoresis/Western Blotting VideoVideos

Meet the Brightest ECL Substrate Around — Clarity Max™ Western ECL Substrate

See how our new high-sensitivity western ECL substrate, Clarity Max, can help you achieve greater western blotting success.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Six Tips for Efficient Transfer of Your Proteins

A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.
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