Discovering Small Vesicles with Flow Cytometry
Cell BiologyFeatured Stories

Flow Cytometry — A Gateway for Discovering More about Small Vesicles

Small vesicles were identified over 50 years ago but scientists are still trying to understand their biology and role in disease. These vesicles have become relevant for monitoring, diagnosing, and treating complex diseases such as cancer. Yet, because of their small size compared to cells, they require intricate technologies to truly understand their impact in research. Learn about the various technologies used to study small vesicles and discover the power of flow cytometry for learning more about these valuable vesicles.
Western Blot Detection of Proteins Present in Tissue Lysates
Electrophoresis/Western BlottingProtocols and Tips

10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates

Extracting proteins from tissue lysates for western blotting can be a challenge. From knowing how to process your tissue to verifying protein loading to including suitable controls, there is a lot to keep track of. The ten tips in this article can help you on your way to generating reproducible and reliable blots and do better western blotting overall.
Brilliant Science Podcast Series
ArticlesDrug Discovery and DevelopmentPodcasts

Brilliant Science Podcast Series

The Brilliant Science podcasts will introduce you to some of the amazing science being done around the world. Join us every month to see what you can learn.
Minimize On-Column Aggregation during mAb Purification
ChromatographyProtocols and Tips

Got Purification Troubles?

Use our interactive infographic to find out how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification. And find out what you can do to minimize these on-column aggregates.
ArticlesElectrophoresis/Western BlottingGeneral Interest

Defining the New Normal in Quantitative Western Blot Data

Normalization of western blot data is very crucial in quantitating proteins. Normalization of band intensity of proteins of interest with the band intensity of housekeeping proteins (HKP) is routinely done in labs. But more and more journals question the validity of using HKPs in normalization and are demanding more validations and tests. An alternative method is to use total protein normalization (TPN) for normalization. Explore the TPN option and see how you can get reliable western blot data easily.
Multiplex Cytokine Immunoassays
ArticlesBio-Plex Multiplex AssaysFeatured Stories

Multiplex Cytokine Immunoassays — Minimizing Effort, Maximizing Results

Since the first cytokine was identified over 60 years ago, a lot has been done to better understand these molecules. The use of multiplex immunoassays is one such way, though they aren’t considered quite as mainstream yet. Learn about how the SMARTS approach can be used to better align user experience to the performance characteristics of these multiplex assays and hopefully transition them to a more mainstream role in research.
A Resin for Purification of Large Biomolecules
ArticlesChromatographyProduct Highlights

A Resin Built Specifically for Large Biomolecule Purifications

Nuvia HP-Q is a high-performance strong anion exchange resin designed for purification of large biomolecules. See how its optimized particle and pore size leads to high target recovery and high-throughput purifications.
Publishing Meaningful Antibody Data
ArticlesCell BiologyElectrophoresis/Western BlottingProtocols and Tips

Finding a Good Antibody:
Part III – Publish Meaningful Data

In the first two articles in our Finding a Good Antibody series, you learned how to select the best antibodies and received tips on proper antibody validation. In this third and final article, see how you can use this information to publish meaningful data when using antibodies. And get some guidelines for antibody best practices.
Single-Cell Analysis and the Endless Possibilities
ArticlesDrug Discovery and DevelopmentPodcasts

Finding New Horizons — What Possibilities Exist with Single-Cell Analysis?

Single-cell techniques offer exciting, new ways to understand personalized medicine and research. Developing these techniques at Bio-Rad is Dr. Kelly Kaihara, the third guest on Brilliant Science. Read about what makes single-cell analysis different and Kaihara’s perspective on its potential moving forward.
ChromatographyPast WebinarsWebinars

Increase Yield and Reproducibility While Saving Time by Automating Recombinant Protein Purification

Presented by: Philip Chapman, MSc, Senior Global Product Manager
Date: Tuesday, July 31, 2018
Time: 8 AM and 6 PM, U.S. Pacific time

Learn how to quickly optimize conditions prior to automating a four-step purification of a histidine-tagged recombinant protein. Automating purification reduces a multi-day process into 12 hours while increasing yield and reproducibility.