Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR
ArticlesFeatured StoriesProtocols and TipsReal-time qPCR/PCR

Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR

One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.
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Multiplex RT qPCR in One Step
ArticlesFeatured StoriesReal-time qPCR/PCR

New Product Feature: Multiplex RT-qPCR in One Step

Using RT-qPCR for RNA, though critical in several industries, is often met with challenges. Many current options don’t allow for multiplexing or high-throughput operation. Bio-Rad’s new Reliance One-Step Multiplex Supermix was designed to grant researchers the expanded, efficient capabilities they need in RT-qPCR. This article presents some of the performance data from the new supermix and describes how exceptional it is at multiplex detection.
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The Universal qPCR Reagent
ArticlesGeneral InterestReal-time qPCR/PCR

One for All — The Universal qPCR Reagent

Passive reference dyes are included in qPCR/RT-qPCR supermixes to compensate for pipetting errors and thermal and/or optical differences in real-time instruments. Our universal line of reagents eliminates the need for spiking in dyes or having to worry about how much ROX your instrument needs. See how your qPCR experiments can also generate more reliable data and be reproducible by anyone.
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Understanding the Long Noncoding Transcriptome
Real-Time qPCR/PCR VideoVideos

Discover lncRNA: Understanding the Long Noncoding Transcriptome

Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).
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