real-time qPCR/PCR

Large sample volumes may sometimes be needed to conduct gene expression studies. Bio-Rad’s new CFX96 Touch Deep Well™ real-time PCR detection system can run sample volumes up to 125 µl and can discriminate up to five targets simultaneously.

Verifying the performance of your real-time PCR system is crucial to obtaining reliable data from your experiments. Bio-Rad’s new CFX qualification plate comes with a quick and easy protocol that provides reliable performance verification of any real-time qPCR system.

Pathway analysis is an efficient approach for studying a large number of related gene targets in a single experiment. Bio-Rad Laboratories and Thomson Reuters, a leading provider of system biology tools, have partnered to provide predesigned pathway panels for gene expression analyses using real-time qPCR. This tech report describes how Bio-Rad’s PrimePCR™ pathway panels were curated and designed using a ranking strategy developed by Thomson Reuters.

The PX1 PCR Plate Sealer efficiently seals microplates used in thermal cycling. This heat sealing method consistently delivers reliable data by helping eliminate sample evaporation during PCR.

The MIQE guidelines have set a new standard for publishing qPCR results to ensure integrity in the scientific literature and increase experimental transparency. One of the key guidelines focuses on the proper design and experimental validation of primer assays used in qPCR. This tech report describes the design and wet-lab validation of PrimePCR assays and how they comply with the MIQE guidelines.

The latest version of the CFX Manager software provides a variety of usability enhancements and powerful new data analyses so users can get the most out of each run.

PrimePCR™ assays set a new standard for real-time PCR. Bio-Rad® collaborated with the leaders in real-time PCR to design, optimize, and experimentally validate every primer pair.

The significant drop in leprosy worldwide is attributed to the development of antimicrobial multidrug therapy (MDT). Resistance to rifampicin — the backbone of MDT treatment — has appeared. Controlling leprosy transmission requires routine surveillance for mutations in the drug target genes before, during, and after the course of treatment. New high resolution melt (HRM) assays offer leprosy researchers a faster, more economical method to investigate resistance targets.

Use the PrimePCR lookup tool to find assays and panels for your genes of interest.

The iTaq™ universal SYBR® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument.