
10 Key Factors for Achieving Accurate Cell Counts: Tips, Tricks, and Myths
Counting is something we are all familiar with but itβs not always as simple as one, two, three. Measuring the concentration of cells in suspension is a common but surprisingly complex task in cell biology that is easy to get wrong. This article explores ten factors that can influence cell counting, gives advice on how to improve accuracy, and dispels some commonly held myths.

Sequenced β Comic Series
Join Terence, a motivated undergrad, as he experiences the ups and downs of research using next-generation sequencing, learning valuable tips for successful NGS experiments along the way

What You Need to Know About Packing Resins at Process Scale
Learn best practices and guidelines for successfully packing different types of chromatography resins in this video webinar. This includes incompressible resins, like CHT Ceramic Hydroxyapatite Media, as well as traditional compressible resins. In general, parameters such as compression factor, settling rate, and attributes of the base matrix need to be considered when packing resins at process scale. Packing methods can be adapted to obtain simple, highly reproducible packing. Get tips here.

Quick Tips for Bio-Plex Assays
It is imperative to plan and prepare ahead of time for your research using Bio-Plex Assays to be successful. Good assay planning and preparation reduces mistakes and makes for a stress-free assay run. In this series of short videos, we share some simple steps you can take to make sure your work goes smoothly.

A Mixed-Mode Approach β An Alternative to a Three-Step mAb Purification Process
Monoclonal antibody (mAb) purification methods often involve the use of resin-based chromatography. In this video, we compare a three-step mAb purification workflow, which uses Nuvia Ion Exchange Resins postβProtein A capture, to a two-step purification workflow, which includes a Protein A capture step followed by a mixed-mode chromatography step using Nuvia aPrime 4A Resin, a hydrophobic anion exchange resin. We present an additional two-step purification approach using Protein A resin followed by a calcium affinity cation exchange media, CHT Ceramic Hydroxyapatite XT Media. The results of both studies demonstrate that a high purity product can be attained using one fewer unit operation than traditional workflows.

Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples
Incorporating preamplification into your qPCR experiments may seem daunting, but it is a powerful way to analyze targets from limited samples. The tips in this article will help you understand how preamplification can enhance your qPCR experiments and the considerations and implications for incorporating it into your workflow.

Tips to Make Fluorophore Picking Easier
Fluorescence is a property of photo-excitable molecules that has been exploited to develop dyes, or fluorophores, that are used in a variety of experimental procedures, including western blotting. Learn more about the science behind fluorescent dyes and get some helpful tips on how to pick the right fluorophores for your experimental needs.

Key Steps to Ensure Optimized and Reproducible qPCR Experiments
Real-time PCR is a common and easy-to-adopt technology. By keeping a few simple steps in mind as you design and run your experiments you can ensure high-quality, reproducible data.

Characterization of Critical Reagents for Ligand Binding Assays
Ligand binding assays are utilized throughout the large-molecule drug development process. However, their robustness, accuracy, and reproducibility depend on the quality of critical reagents. See how you can ensure their consistent characterization, particularly during early drug development stages.

Multiplex Immunoassays: Five Tips for Optimizing Your Experiments
Follow the five tips in this article before embarking on your multiplex immunoassay journey to generate accurate, efficient, and biologically relevant results. From help designing experiments to acquiring accurate data, this article has it all.