Bio-Rad’s Downstream Process Development Support and Services
Bio-Rad’s Chromatography Support Scientist, Artur Stanczak, discusses the company’s comprehensive downstream process development services. From method development to troubleshooting, Bio-Rad provides specialized support to help customers overcome bioprocessing challenges. Access tools, case studies, and expert guidance to optimize processes and advance biomanufacturing efforts in this informative video.
Development of an IEX Purification Process for Lentiviral Vectors
In recent years, gene therapy treatments have increased significantly, requiring more efficient processes to purify and deliver these therapeutics. Lentiviral vectors (LVVs) are a type of retrovirus and are an effective tool for gene delivery. In this webinar, data are shown for a high-throughput screening strategy that was employed for LVV purification, which provided high yield as well as acceptable host cell protein (HCP) clearance.
Developing Efficient Downstream Purification Processes for Bispecific Antibodies
Modern chromatographic resins enable new strategies for downstream processing of bispecific antibodies. Watch this webinar to see case studies and workflows.
A Polishing Strategy for Removing Impurities in Bispecific Antibody Purification
Bispecific antibodies pose unique challenges in downstream processing. See how impurities were efficiently removed by mixed-mode chromatography in this webinar.
mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach
In this case study, process chromatography application scientist Artur Stanczak presents a two-step mAb purification workflow, comparing multimodal resin alternatives used for the polishing step that occurs after protein A capture. The design of experiment (DOE) data presented characterizes the behavior of multiple mAbs with two different mixed-mode resins. The importance of scale, prior knowledge, and the stage of development will also be discussed. The results of both mixed-mode resin studies and the different DOE approaches demonstrate the ability to achieve high-purity products.
Assessing Viral Clearance in Early-Phase Process Development
Viral clearance studies are part of a multifaceted approach to ensure biopharmaceutical product safety. To prevent costly changes to a manufacturing process, it is important to assess each operation unit for its efficiency early on during downstream process development. See how a design of experiments (DOE) approach was utilized in this case study to investigate the effect of buffer pH and conductivity on the removal of impurities by an anion exchange (AEX) mixed-mode chromatography resin.
mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach
This webinar shows a 2-step mAb purification workflow and compares multimodal options for polishing. See how these methods can achieve high-purity products.
Downstream Process Development for the Purification of Biosimilar Insulin from Escherichia coli
This webinar presents a downstream processing workflow for recombinant human insulin production from E. coli. Learn how proinsulin and insulin can be purified.
Purification of DNA Oligonucleotides using Anion Exchange Chromatography
In this webcast, see how Nuvia HP-Q Resin, an anion exchange resin, was used to purify oligonucleotides with phosphodiester backbones in a scaleable workflow.
The Process of Resin Screening
Process development scientists, faced with an increasing number of proteins or complex biomolecules, continuously search for robust purification methods that can be rapidly scaled for manufacturing. What are the optimal purification conditions for high throughput and yield? Which resin or resin combination best purifies the desired protein? How can an efficient, yet cost-effective process be established? The answer: use a systematic screening approach. Read on to learn more.