How to Design Hydrolysis Probes for qPCR
What’s the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5′ guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.
Top Stories of 2015 — Bioradiations Year in Review
In 2015, we published an extensive array of tips, protocols, videos, articles of general interest, customer testimonials, and technical and application notes on bioradiations.com. Here is a list of the most popular 2015 stories.
So, How Can the Sso7d Fusion Polymerase Technology Help Your PCR?
During a PCR reaction, it is imperative for the DNA polymerase to remain attached to the template while extending the new strand. This association is jeopardized under challenging PCR conditions and when amplifying longer DNA fragments. See how Sso7d, a small protein capable of binding with the polymerase, helps in stabilizing this association and increasing PCR efficiency.
Flow Cytometry: Basic Definitions
It’s always good to get the basics straight. Here, we’ve defined some flow cytometry terms that might have eluded you.
Six Tips for Increasing the Reproducibility of qPCR Experiments
Follow these six simple tips and you’ll increase the reproducibility of your qPCR experiments.
High-Processivity Sso7d Fusion Polymerase Improves qPCR Results
Processivity is the rate at which nucleotides are added to extend PCR products. High processivity results in a greater number of nucleotides incorporated in each polymerase binding event.
Bio-Rad’s PrimePCR™ Assays and Panels Eliminate Multiple Steps from Your qPCR Workflow
A qPCR workflow involves multiple steps, each of which adds to the time and cost of the entire experiment. The PrimePCR™ Assays and Panels from Bio-Rad eliminate a number of these steps with their predesigned primers and probes and help with the assay design. See what steps they eliminate and how that would help expedite your qPCR experiment.
In Pursuit of (Ig) Nobility: The Ig Nobel Prize
What is so noble about receiving an award? What if it is an Ig Nobel award? This article reviews what Ig Nobel awards are, how they are determined, and discusses some research topics that were awarded the Ig Nobel awards this year.
10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels
Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.
How to Minimize Contamination in qPCR Experiments
Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We’re sharing seven tips for minimizing contamination of qPCR experiments.