Articles

Immunoprecipitation (IP) experiments and the detection of IP samples by western blotting can be quite challenging at times. The tips in this article help you set up and optimize your overall IP experimenta design.

Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.

Part III of our cell health series describes the three main autophagy mechanisms and reviews the various methods for assessing macroautophagy and autophagic flux in cells.

A well-designed multidimensional (Multi-D) chromatography process, which utilizes optimized individual column methods, can help automate multistep protein purification processes. This can help you save time and increase productivity. See how you can use Multi-D chromatography to expedite the purification of polyhistidine-tagged proteins when using a typical capture, intermediate, and polish purification workflow.

CRISPR has revolutionized gene editing but is not the only way to edit a gene. We compare the pros and cons of four main gene editing techniques – meganucleases, zinc finger nucleases, TALENs, and CRISPR-Cas9 – discuss the advance of these methods to the clinic for therapeutic applications, and introduce newer technologies that eliminate some of the pitfalls of current techniques.

Monocytes play key roles in mediating innate and adaptive immune responses. Here is an overview of the types, functions, process of recruitment, and the role of monocytes in disease.

A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.

Striving for efficiency in workflow is not a new concept. Automation and use of smart technologies in industries such as automotive and shipping, and services like USPS and UPS, have drastically changed the way they function today not just with innovation but with personalization. Pharma is beginning to understand the need for adopting personalized workflow as well. Now, what can Pharma learn from these industries and how can it apply it to the industry?

In this second part of the series on assessing the health of your cells, we review the different types and stages of apoptosis, and the various methods of assessing apoptosis of cells.

Macrophages are important players in the phagocytosis process in the immune system. In this detailed review, we provide an overview on the different types of macrophages, the signaling molecules involved in the polarization of M1 and M2 macrophage subsets, and more.