Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting
2-D electrophoresis followed by immunodetection of protein spots using western blotting is adopted commonly in proteomics studies. Here we present step-by-step instructions for obtaining accurate overlaps between the gel and the blot using the PDQuest 2-D gel analysis software.
Bio-Rad’s 2-D electrophoresis workflow offers better results in a shorter time. Take a detailed look at this workflow by downloading the updated edition of our How-To Guide.