ArticlesBio-Plex Multiplex AssaysProtocols and Tips

8 Tips for Understanding Multiplex Immunoassay Development and Validation

Multiplex immunoassay development requires a lot of validation and standardization steps. Several factors can influence the performance of these assays. Some factors that need to be considered are mentioned here.
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ArticlesBio-Plex Multiplex AssaysProtocols and Tips

List of Mouse Lineage Biomarkers for Different Immune Cell Types

How can we easily identify biomarkers for different immune cell types? This color-coded poster makes identification of mouse lineage biomarkers easy.
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ArticlesChromatographyProtocols and Tips

Chromatography: Everything You Need to Know about Your Peaks

What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.
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ArticlesCell BiologyProtocols and Tips

Flow Cytometry: Basic Definitions

It’s always good to get the basics straight. Here, we’ve defined some flow cytometry terms that might have eluded you.
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ArticlesCell BiologyProtocols and Tips

10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels

Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.
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ArticlesCell BiologyProtocols and Tips

Protocol: Preparation of Cells for Flow Cytometry

Harvesting of tissue culture cells could be challenging. Here is an easy protocol for efficient harvesting of cells for flow cytometry analysis.
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ArticlesGeneral InterestProtocols and Tips

Get Published! 10 Tips for Getting Published Successfully

When you have finished all your experiments and have the paper ready in your head, you need to consider several factors before sending it for publication. Here are some tips that Bio-Rad’s PrimePCR™ PCR Primers and Assays group put together for getting the paper published successfully.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

7 Tips for Choosing the Right Antibody for Western Blotting

Which antibody is the best to obtain a definitive and conclusive answer for my questions? How do I choose the best antibody for my purpose? These are standard questions that scientists ask time and time again. Here are a few tips to choose the most appropriate antibody for your specific application.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol

Immunoprecipitation (IP) is a commonly used protocol for concentrating and purifying proteins using specific antibodies and magnetic beads. Here is a simple IP protocol using Bio-Rad’s SureBeads Magnetic Beads.
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ArticlesProtein Interaction AnalysisProtocols and Tips

Ligand Immobilization in Protein Interaction Studies — An Unattended Amine Coupling Protocol with Automatic Coinjection Activation

Amine coupling is the most commonly adopted technique to bind proteins to biosensor chips. Because of the possibility of the amine coupling reagents reacting with each other, these have to be premixed just before the injection and injected independently. Here are some tips to automatically coinject these reagents.
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