Customer Testimonial VideoReal-Time qPCR/PCR VideoVideos

Using PrimePCR™ qPCR Assays to Understand Breast Cancer Metastasis

Traci Lyons describes how PrimePCR Assays ended her laboratory’s struggle to find a qPCR assay that could reliably detect transcription of the cyclooxygenase-2 (Cox-2) gene, which they had identified as potentially prometastatic. Using the PrimePCR Assay for Cox-2, researchers in her lab were finally able to verify cyclooxygenase-2 knockdown, which is a necessary step toward confirming its role in breast cancer metastasis.
READ MORE →
Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

How to Design Hydrolysis Probes for qPCR

What’s the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5′ guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.
READ MORE →
Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

Six Tips for Increasing the Reproducibility of qPCR Experiments

Follow these six simple tips and you’ll increase the reproducibility of your qPCR experiments.
READ MORE →
Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

How to Minimize Contamination in qPCR Experiments

Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We’re sharing seven tips for minimizing contamination of qPCR experiments.
READ MORE →
Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

How to Design Primers for qPCR

How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.
READ MORE →
Customer Testimonial VideoReal-Time qPCR/PCR VideoVideos

High Quality Gene Expression from Precious Samples
— A Simplified Workflow

Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad’s SingleShot Cell Lysis Kit, PreAmp Supermix, and PrimePCR validated primer panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.
READ MORE →
Bench PartnersBench Partners Video SeriesReal-Time qPCR/PCR VideoTips and Techniques VideoVideos

PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate

One easy way to obtain a PrimePCR plate customized for your research is to modify one of Bio-Rad’s predesigned panels. This short video tutorial shows you how to use the online tools at Bio-Rad.com to edit a predesigned plate and create, then order your own customized 96- or 384-well PCR plate.
READ MORE →
Bench PartnersBench Partners Video SeriesReal-Time qPCR/PCR VideoTips and Techniques VideoVideos

How to Build a Custom PrimePCR™ Real-Time PCR Plate Using a Suggested or Blank Template

It’s fast and easy to build your own PrimePCR plate. This video walks you through using the Custom Plate Configurator at Bio-Rad.com to create your own 96- or 384-well PCR plate.
READ MORE →
Bench PartnersBench Partners Video SeriesReal-Time qPCR/PCR VideoTips and Techniques VideoVideos

How to Order PrimePCR™ Assays – Primers, Probes, and Panels

A wide range of PCR assays is available through Bio-Rad.com for use with real-time or Droplet Digital™ PCR systems. Get a quick refresher on ordering these products with this short video.
READ MORE →
Customer Testimonial VideoReal-Time qPCR/PCR VideoVideos

T100™ Thermal Cycler – Endorsed by Customers around the World

Life science researchers all over the world use the T100 thermal cycler in their daily routine. In this video, users describe how it saves them time and helps them consistently obtain high-quality data.
READ MORE →