A Resource for Life Science Research

Announcements

Science Ambassadors Program Reaches New Age Groups, More Schools
Bio-Rad Science Ambassador Program Reaches New Age Groups, More Schools

The Science Ambassador Program continues to grow in its mission to connect working scientists with students at the elementary, middle, and high school level. This month, bioradiations catches up with Bio-Rad’s corporate citizenship initiative at two events: the USA STEM festival in Washington, D.C., and a special day of science in Bio-Rad’s home town, Hercules, CA.

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Brighter Precision Plus Protein™ Dual Color Standard — The Brightest Protein Marker Available

Take a quick look at Bio-Rad’s brighter Precision Plus Protein™ Dual Color Standard with this article comparing this standard to other available protein markers. Plus, find out why the ability to visualize a protein standard after reprobing matters to one researcher!

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Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips

Bio-Rad’s Universal Real-Time PCR Kits and Reagents work on all real-time PCR systems. Now you can easily compare performance data and get supporting documents for these kits and reagents using Bio-Rad’s new tablet and web app. Learn more and download the app here.

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In The Field

Trends in Protein Separation and Analysis — the Advance of Stain-Free Technology
Trends in Protein Separation and Analysis — the Advance of Stain-Free Technology

What is stain-free technology? How does it work in electrophoresis and western blotting? How does it compare with existing staining techniques? What are its advantages and possible concerns? Here is a technical review of this rapidly growing technological advance.

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Purification Trends: How Smart is Chromatography Automation?

What does automated protein purification mean today? What might it look like tomorrow? Bioradiations talks to a variety of users about what they’re doing now to implement automation in their purification processes, and what possibilities they envision that will improve chromatography in the near future.

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Genomic Variation Analysis with Droplet Digital™ PCR: Success Stories

Researchers are having success applying Droplet Digital PCR (ddPCR™) technology across many different fields. Here are two such success stories, one involving the application of ddPCR in detecting mutations in iPS cells, the other in predicting allele-specific variation in human brain development.

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Tips and Techniques

Bench Partners
Responding to Common Error Messages When Using the Bio-Plex® 200 System

These short videos demonstrate how to troubleshoot some of the most common error messages that occur in operating the Bio-Plex® 200 System.

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DIY Cell Sorting with Bio-Rad’s S3™ Cell Sorter

Until recently, cell sorting has been a complex endeavor requiring specialized facilities and trained technicians. This infographic reveals how Bio-Rad’s S3 cell sorter makes this technique available to individual, non-expert researchers in 3 easy steps.

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Bio-Rad TC20™ and TC10™ Cell Counters – Cleaning the Print Heads for Clear Printed Results

Most problems with misaligned printouts on the printer supplied with the TC20 and TC10 cell counters can be solved by cleaning the print heads. In this short video, find out the fast, easy, and effective way to clean these print heads so you can get back to your research!

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Solutions

The Ever-Expanding Universe of Droplet Digital PCR Applications
The Ever-Expanding Universe of Droplet Digital PCR Applications

Bio-Rad’s groundbreaking Droplet Digital™ PCR technology can be applied in a variety of applications to glean precious information that cannot be obtained by any other method. Find out more about how this technology has been used in applications such as quantification of cancer biomarkers, validation of next generation sequencing, distinguishing genomic variation, and more.

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Profiling Dynamic Cancer Drug Responses with BioMarker Strategies, LLC and the Bio-Plex Multiplex Immunoassay System

In an age of personalized medicine, getting the most out of highly targeted cancer therapies takes finding the best molecular targets for each patient. BioMarker Strategies LLC, a small startup, has been developing an innovative method to do this by modeling oncogenic pathway responses, but they needed to accurately measure the levels of phosphoproteins resulting from the activation of these genes. Learn how they are using the Bio-Plex multiplex immunoassay system to accurately quantify the proteins and generate functional signaling profiles for these oncogenes.

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Building New Anti-HIV Molecules with Bio-Rad's ProteOn™ XPR36 System

Can we construct molecules that will interfere with disease pathways? Dan Mitchell of the University of Warwick and his colleagues wanted to apply new synthesis techniques to interrupting HIV infection. Find out what they learned about the potential of these novel molecules using Bio-Rad's ProteOn™ XPR36 System.

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Technical Reports

Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro™ RBM Apoptosis Assays
Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro™ RBM Apoptosis Assays

Bio-Rad’s new Bio-Plex Pro RBM Apoptosis Multiplex Assays enable complex, quantitative analyses of biomarkers in the intrinsic apoptosis pathway. Learn more about these biomarkers and view sample data and performance characteristics of these assays in this detailed technical report. Read More

 
Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer
Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer

Conducting a triplex one-step RT-qPCR assay promises a more efficient and economical analysis of gene expression compared to running three individual assays. This technical report describes a colorectal cancer case study where a triplex multiplex assay was validated. Read More

 
Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System
Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System

While using affinity tagging is the preferred method to achieve highly pure protein through recombinant expression, this approach isn’t feasible for all proteins. This tech report describes workflow optimizations using the NGC Chromatography System that allow users to achieve high purities with untagged proteins. Read More

 
Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction Using the ProteOn™ XPR36 System
Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction Using the ProteOn™ XPR36 System

Surface plasmon resonance (SPR) biosensors offer a label-free technique for profiling biomolecular interactions, including those between drugs and liposomes. Here we describe the novel surface chemistries that make capturing liposomes possible, and explore the application of the liposome capturing kit in analyzing liposome-small molecule interactions. Read More

 
Applications of Bio-Plex Pro™ Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways
Applications of Bio-Plex Pro™ Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways

The Akt and MAPK signal transduction pathways are key areas of study for their roles in cell growth, function, and death, as well as for their possible roles in cancer development and diabetes. Bio-Rad's new Bio-Plex Pro™ Akt and MAPK cell signaling assays enable researchers to study the activation of these key pathways as a response to various stimuli. This technical report validates the sensitivity and specificity of these assays after cells were exposed to insulin, EGF (epidermal growth factor), and UV radiation. Read More

 
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