A Resource for Life Science Research

Announcements

Bio-Rad's FastCast™ Acrylamide Kits — It's Time to Expect More from Your Handcast Gels
A New Breed of Cell Sorters: Bio-Rad’s S3™ Cell Sorter Appeals to the Growing Cell Biology Community

The S3 cell sorter is Bio-Rad’s new addition to the world of flow cytometry. Take a look at how this instrument breaks the mold for cell sorters, brings relief to investigators and core lab managers alike, and opens up exciting new research possibilities.

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Bio-Rad's FastCast™ Acrylamide Kits — It's Time to Expect More from Your Handcast Gels

Hand casting polyacrylamide gels is not an easy task. It is laborious, needs lot of pre-planning and is cumbersome. Bio-Rad's new TGX and TGX Stain-Free FastCast gel solutions not only address all these issues, but provide added benefits, such as increased speed, stain-free visualization, and quality control. Learn more about these new handcasting kits.

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The Chromatography Chronicles Part 5: Chromatography You Can Count On

In biochromatography, every purification or separation procedure that fails because of instrument malfunction wastes precious lab resources. That’s why Bio-Rad, in designing the NGC chromatography system, found new ways to engineer reliability into the chromatography experience. Here’s how they did it.

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In The Field

Transforming Cancer Research: Droplet Digital™ PCR Plays Integral Role at Fred Hutchinson Cancer Research Center

Droplet Digital PCR has major implications for cancer studies, where the ability to accurately measure biomarkers may result in important insights into cancer genesis and progression. Find out about new findings on micro RNA and Tumor Infilitrating Lymphocytes made possible by ddPCR technology.

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Microbes in Space! High School Students Design Experiments for the International Space Station with Bio-Rad's pGLO™ Bacterial Transformation Kits

Retired satellite engineer Dan Saldana is helping Valley Christian High School in San Jose, CA build a program to send student experiments to the International Space Station. To study microbial growth in microgravity, Saldana and his students turned to Bio-Rad's pGLO bacterial transformation kits. Learn about the high-flying travels of these glowing bacteria and the science learning that resulted.

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Labs for Inspiration: Making a Difference with Bio-Rad's Science Ambassador Program

Jefferson County, Tennessee, faced tight school budgets and a mandate to "teach to the test" that left little room for hands-on science learning. Learn how biology professor Lisa Eccles worked to reverse this trend by getting involved with the Science Ambassador program, Bio-Rad's fast-growing initiative to spark excitement about life science in primary school classrooms.

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Tips and Techniques

Efficient SPR-Based Fragment Screening and Small Molecule Affinity Analysis Using Bio-Rad's ProteOn™ XPR36 System
DIY Cell Sorting with Bio-Rad’s S3™ Cell Sorter

Until recently, cell sorting has been a complex endeavor requiring specialized facilities and trained technicians. This infographic reveals how Bio-Rad’s S3 cell sorter makes this technique available to individual, non-expert researchers in 3 easy steps.

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Bench Partners
Bio-Rad TC20™ and TC10™ Cell Counters – Cleaning the Print Heads for Clear Printed Results

Most problems with misaligned printouts on the printer supplied with the TC20 and TC10 cell counters can be solved by cleaning the print heads. In this short video, find out the fast, easy, and effective way to clean these print heads so you can get back to your research!

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Bio-Rad Supply Center — How-To Tips for Setting Up and Taking Inventory

In this new video series, Bio-Rad offers answers for some your most frequently asked questions. This installment covers setting up and taking inventory of your Bio-Rad Supply Center.

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Solutions

The Ever-Expanding Universe of Droplet Digital PCR Applications
The Ever-Expanding Universe of Droplet Digital PCR Applications

Bio-Rad’s groundbreaking Droplet Digital™ PCR technology can be applied in a variety of applications to glean precious information that cannot be obtained by any other method. Find out more about how this technology has been used in applications such as quantification of cancer biomarkers, validation of next generation sequencing, distinguishing genomic variation, and more.

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Profiling Dynamic Cancer Drug Responses with BioMarker Strategies, LLC and the Bio-Plex Multiplex Immunoassay System

In an age of personalized medicine, getting the most out of highly targeted cancer therapies takes finding the best molecular targets for each patient. BioMarker Strategies LLC, a small startup, has been developing an innovative method to do this by modeling oncogenic pathway responses, but they needed to accurately measure the levels of phosphoproteins resulting from the activation of these genes. Learn how they are using the Bio-Plex multiplex immunoassay system to accurately quantify the proteins and generate functional signaling profiles for these oncogenes.

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Building New Anti-HIV Molecules with Bio-Rad's ProteOn™ XPR36 System

Can we construct molecules that will interfere with disease pathways? Dan Mitchell of the University of Warwick and his colleagues wanted to apply new synthesis techniques to interrupting HIV infection. Find out what they learned about the potential of these novel molecules using Bio-Rad's ProteOn™ XPR36 System.

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Technical Reports

Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction Using the ProteOn™ XPR36 System
Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction Using the ProteOn™ XPR36 System

Surface plasmon resonance (SPR) biosensors offer a label-free technique for profiling biomolecular interactions, including those between drugs and liposomes. Here we describe the novel surface chemistries that make capturing liposomes possible, and explore the application of the liposome capturing kit in analyzing liposome-small molecule interactions. Read More

 
Applications of Bio-Plex Pro™ Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways
Applications of Bio-Plex Pro™ Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways

The Akt and MAPK signal transduction pathways are key areas of study for their roles in cell growth, function, and death, as well as for their possible roles in cancer development and diabetes. Bio-Rad's new Bio-Plex Pro™ Akt and MAPK cell signaling assays enable researchers to study the activation of these key pathways as a response to various stimuli. This technical report validates the sensitivity and specificity of these assays after cells were exposed to insulin, EGF (epidermal growth factor), and UV radiation. Read More

 
Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins
Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins

Removing host cell protein (HCP) contaminants represents an essential step in the production of biologics (therapeutic protein drugs), but has long been a time- and labor-intensive process. Here, we demonstrate a simpler, quicker, and more standardized workflow for evaluating anti-HCP antibodies using 2-D electrophoresis and western blotting. Read More

 
Immobilization of Active Kinases for Small Molecule Inhibition Studies
Immobilization of Active Kinases for Small Molecule Inhibition Studies

Surface plasmon resonance (SPR) is a well-established and important screening tool in the small molecule drug discovery workflow. Using the Bio-Rad SPR system ProteOn™ XPR36, rapid optimization of immobilization conditions for a kinase target was carried out in a fast workflow with a single sensor chip, showing the high performance of the ProteOn XPR36 system. Read More

 
PrimePCR™ Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy
PrimePCR™ Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy

Pathway analysis is an efficient approach for studying a large number of related gene targets in a single experiment. Bio-Rad Laboratories and Thomson Reuters, a leading provider of system biology tools, have partnered to provide predesigned pathway panels for gene expression analyses using real-time qPCR. This tech report describes how Bio-Rad's PrimePCR™ pathway panels were curated and designed using a ranking strategy developed by Thomson Reuters. Read More

 
 
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