flow cytometry

Presented here are some tips to help you avoid buyer’s remorse and make an educated decision about which instrument to purchase. These tips provide you with the tools to make an informed decision when purchasing a cell sorter.

Fluorescence-activated cell sorting is a valuable tool for research, but could the physical forces of sorting stress the cells, altering their behavior and confounding experimental results? Recent research found that although cell sorting activated p38 MAPK stress signaling, no functional or structural changes were observable. The results indicate that jet-in-air systems such as the Bio-Rad S3e Cell Sorter do not alter cell behavior.

Incorporating fluorescence-based cell sorting into your lab grants you powerful access to new research areas and rarer cell types without sacrificing efficiency or results. New, powerful techniques like CRISPR and single-cell analysis are enhanced by the isolation of specific cells. But isolating cell populations of the right quality and quantity is both challenging and time consuming. We offer some tips to set yourself up for success and get the most out of your sorts.

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.

Presented by: Angie Green, PhD, Senior Scientist

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We discuss using a multiparameter flow cytometry panel to monitor T-cell exhaustion states and the expression of checkpoint inhibitors. We used an in vitro model; however, the panel is designed to be compatible with clinical samples to monitor immunotherapy.

Presented by: Mike Blundell, PhD, Product Manager

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Flow cytometry is a powerful technique used widely in biological research, but if you are just beginning your flow journey, it can be hard to know where to start. In this webinar, you will:

• Learn the important steps to obtaining better flow cytometry data
• Get advice on how to apply this knowledge to improve your experiments
• Find out how Bio-Rad’s panel builder tool can help you design your experiments, no matter how many colors you plan to use

Small vesicles were identified over 50 years ago but scientists are still trying to understand their biology and role in disease. These vesicles have become relevant for monitoring, diagnosing, and treating complex diseases such as cancer. Yet, because of their small size compared to cells, they require intricate technologies to truly understand their impact in research. Learn about the various technologies used to study small vesicles and discover the power of flow cytometry for learning more about these valuable vesicles.

In the first two articles in our Finding a Good Antibody series, you learned how to select the best antibodies and received tips on proper antibody validation. In this third and final article, see how you can use this information to publish meaningful data when using antibodies. And get some guidelines for antibody best practices.

Immuno-oncology, or cancer immunotherapy, research focuses heavily on the various types of cells and their behavior in cancer, so the use of flow cytometry to interrogate these cells is a no brainer. See how flow cytometry fits in this growing field of research and how it can help identify new targets and improve drug design to generate more effective and safer cancer therapeutics.

Finding antibodies that work for your application is just the first step in finding a good antibody. Next is antibody validation — making sure that your antibody does what you think it is supposed to. In this second article in our Finding a Good Antibody series, learn how best to go about validating your antibodies.