Chromatography VideoVideos

A Mixed-Mode Approach — An Alternative to a Three-Step mAb Purification Process

Monoclonal antibody (mAb) purification methods often involve the use of resin-based chromatography. In this video, we compare a three-step mAb purification workflow, which uses Nuvia Ion Exchange Resins post–Protein A capture, to a two-step purification workflow, which includes a Protein A capture step followed by a mixed-mode chromatography step using Nuvia aPrime 4A Resin, a hydrophobic anion exchange resin. We present an additional two-step purification approach using Protein A resin followed by a calcium affinity cation exchange media, CHT Ceramic Hydroxyapatite XT Media. The results of both studies demonstrate that a high purity product can be attained using one fewer unit operation than traditional workflows.
READ MORE →
Featured WebinarsWebinars

Generation of Recombinant Tool Antibodies to Support Cell and Gene Therapy Development

Recombinant antibodies, CAR-T Cell Therapy Development, CAR-T Cell Therapy, Monoclonal Antibody Generation, custom antibodies, cell and gene therapy development, bioanalysis, biosimilar, immunogenicity, drug monitoring, antibody drug conjugate.
READ MORE →
ArticlesDroplet Digital PCR (ddPCR)General Interest

Bispecific Antibodies and Cancer Immunotherapy

Bispecific antibodies (bsAbs) are an important addition to the immuno-oncology toolbox. Designed to recognize two distinct epitopes, bsAbs have enhanced binding, specificity, and efficacy compared to current monovalent antibody therapeutics, making them exciting candidates for more targeted cancer treatments. Learn about bsAbs and how our tools can help scientists with their research on therapeutic candidates.
READ MORE →
ArticlesCell Biology

5 Reasons to Read the Antibody Advice Guide

In the day-to-day pressures of a research environment, it is natural to want to generate important data as quickly as possible. However, there are real benefits in ensuring that the antibody you have picked is the best for your experiment and spending time aliquoting and titrating your antibodies. Bio-Rad recently launched a free Antibody Advice Guide to equip scientists with the knowledge to get the best out of their experiments with antibodies. Read on for five examples of valuable information that you will learn from the guide, helping you generate publication-ready data.
READ MORE →
Electrophoresis/Western Blotting VideoVideos

Choosing Validated Antibodies for Trustworthy Data

The lack of reproducibility in research has been a source of frustration for scientists. Learn why antibody validation is critical for ensuring specificity and what validation methods Bio-Rad is implementing. Our scientists validate the performance of antibodies through CRISPR-Cas9 gene editing by knockout (KO), siRNA knockdown (KD), and immunoprecipitation followed by mass spectrometry (IP-MS).
READ MORE →
ArticlesChromatography VideoCustomer Stories

Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems

See how an antibody expression service provider, Evitria (Zurich, Switzerland) optimized and expanded their antibody production services using the NGC Chromatography Systems to meet the growing demands of their customers.
READ MORE →
R-Loops in Cancer Research
ArticlesCell BiologyDrug Discovery and Development

R-Loops and Their Key Role in Cancer Research

R-loops are being investigated as potential therapeutic targets for cancer. Find out what they are, how they form, how their formation is controlled by our cells, and, above all, their potential as therapeutic targets.
READ MORE →
Cell BiologyPast WebinarsWebinars

To Boldly Flow: Gain Confidence to Start Flow Cytometry

Presented by: Mike Blundell, PhD, Product Manager

View on demand

Flow cytometry is a powerful technique used widely in biological research, but if you are just beginning your flow journey, it can be hard to know where to start. In this webinar, you will:

  • Learn the important steps to obtaining better flow cytometry data
  • Get advice on how to apply this knowledge to improve your experiments
  • Find out how Bio-Rad’s panel builder tool can help you design your experiments, no matter how many colors you plan to use

READ MORE →
ArticlesGeneral InterestGeneral Interest Video

Discover the Technology behind Bio-Rad’s Custom Recombinant Antibodies

See inside Bio-Rad’s custom antibody facility and learn how specific antibody requirements can become a reality through the use of Human Combinatorial Antibody Library (HuCAL®) technology for specialized custom antibodies.
READ MORE →