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Single-Step Column Purification for H1N1 Virus
Using Nuvia HP-Q AEX Resin

Learn how a team developed a single-step workflow for column purification of the Influenza A H1N1 subtype using the recently developed Nuvia HP-Q Anion Exchange Resin. This protocol is scalable, efficient, and produced good yields with high purity.
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Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems

See how an antibody expression service provider, Evitria (Zurich, Switzerland) optimized and expanded their antibody production services using the NGC Chromatography Systems to meet the growing demands of their customers.
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Discover Our Capabilities

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.
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Chromatography VideoVideos

Coupling Automated Chromatography with Faster Confirmation of Purity and Yield

We’ve combined the chromatography process with purity and yield assessment using stain-free gels to shorten and optimize your protein purification workflow. See how it’s done.
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Attaching a Dynaloop to the NGC™ Chromatography System

This video describes the three main steps in attaching a DynaLoop Sample Loop to the NGC™ Chromatography System to enable the injection of larger sample volumes: connecting the DynaLoop Sample Loop to the NGC Chromatography System, priming the DynaLoop Sample Loop, and creating a method in ChromLab™ Software.
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Calibrating the pH Valve on the NGC™ Chromatography System

The NGC pH valve provides real-time, in-line monitoring of buffer pH. In this video, you’ll learn how to connect the pH valve to the NGC System, add it to the flow path, and calibrate the valve using buffers at two different pH values. Features of the pH valve include accessible calibration ports and easy bypass of the probe when the NGC System is being cleaned.
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Removing Air Bubbles from the NGC™ Chromatography System

This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles.
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Using the Air Sensor on the NGC™ Chromatography System

This video describes the functions of the Air Sensor Module and how to connect it to the NGC Chromatography System. The NGC Air Sensor senses the end of sample or buffer and prevents the introduction of air into the system, which can damage columns and/or incur the loss of precious samples. Both small and large air sensors are available to fit the tubing being used. Steps for connecting the Air Sensor Module and associated air sensors are described including how to add the air sensors into the system flow path using ChromLab™ Software and the differences in using the air sensors in manual vs. method modes.
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How Do You Automate a Multicolumn Protein Purification?

Multicolumn purifications are tedious and the manual operations during this process introduce variabilities, affecting the success of purification and reproducibility of results. This whiteboard animation explains how an automated multi-dimensional chromatography method can make this an easier process with very high success rates.
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How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution?

The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.
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