Droplet Digital PCR (ddPCR)

Researchers are having success applying Droplet Digital PCR (ddPCR™) technology across many different fields. Here are two such success stories, one involving the application of ddPCR in detecting mutations in iPS cells, the other in predicting allele-specific variation in human brain development.

Bio-Rad’s groundbreaking Droplet Digital^™ PCR technology can be applied in a variety of applications to glean precious information that cannot be obtained by any other method. Find out more about how this technology has been used in applications such as quantification of cancer biomarkers, validation of next generation sequencing, distinguishing genomic variation, and more.

Droplet Digital PCR has major implications for cancer studies, where the ability to accurately measure biomarkers may result in important insights into cancer genesis and progression. Find out about new findings on micro RNA and Tumor Infilitrating Lymphocytes made possible by ddPCR technology.

Prof. Alec Morley, a pioneer in digital PCR, describes his use of Droplet Digital PCR (ddPCR™) technology to advance the diagnosis and treatment of leukemia. Morley and his colleagues at Flinders University and Medical Center in Adelaide, South Australia previously developed a digital PCR method for the early detection of residual acute lymphoblastic leukemia (ALL). Learn how Morley is now using Bio-Rad’s ddPCR technology to develop a diagnostic test for monitoring chronic myeloid leukemia.

In the early 1990s, researcher Alec Morley and his colleagues pioneered digital PCR techniques to measure acute lymphoblastic leukemia. Today, Morley is trying to develop a more accurate way to quantify chronic myeloid leukemia. Find out why he chose Bio-Rad’s Droplet Digital PCR technology for this effort.

The QX100 Droplet Digital PCR system gives researchers an improvement in PCR precision and sensitivity. See how this innovative digital PCR system is enabling companies to create better products, improve workflow efficiencies, and validate results with greater accuracy than previous methods allowed.

Researchers at Sangamo BioSciences, Inc. explain the benefits of using third generation PCR as it applies to their research in the field of personalized medicine. Watch to see the latest generation of quantitative PCR in action.

Sangamo BioSciences, Inc., located in Richmond, California, is developing HIV therapeutics toward a functional cure using their proprietary zinc finger DNA-binding protein technology. Droplet digital PCR (ddPCR™) — or third generation PCR — offers a superior solution as it can detect target sequence within a single molecule, making it a powerful technology for low copy event detection.

Watch the step-by-step workflow for absolute quantification of DNA molecules using the third generation droplet digital PCR technology.

Droplet digital PCR is considered the third generation of PCR technology. This technology enables the quantification of absolute measure of target DNA molecules, rather than the relative measure obtained from real-time PCR. Absolute quantification is advantageous in several applications, such as measuring copy number variation, detecting rare sequences and mutations, and analyzing gene expression.