Articles

Genetic screens are powerful tools for finding genes associated with a particular phenotype. They play an especially important role in cancer research and are leveraged to identify predictive biomarkers that can be used to improve treatment efficacy or trace the progression of genetic alterations that ultimately lead to disease. The HAP1 cancer cell line model and fluorescence-activated cell sorting (FACS) bring efficiency to the development of genetic screens that is making them a force to be reckoned with.

Developing adeno-associated virus (AAV)-mediated gene therapies requires accurate and precise quality control. Read how Droplet Digital PCR technology provides the sensitivity needed to assess the potential safety and effectiveness of these treatments.

The COVID-19 pandemic has captured the attention of the world and won’t let go. And that has sparked efforts for creative solutions in some surprising places. Wastewater is not where most peoples’ minds go first when they think of airborne diseases, but it holds much promise for tracing and predicting SARS-CoV-2 outbreaks.

Mice are critical model organisms for the study of neuroinflammation. But tissue quantity is often a limiting factor. In a recent paper, researchers describe a protocol for analysis of 33 cytokines and chemokines using only 50 μl of tissue homogenate. The remaining homogenate can be used for RNA extraction or other analysis.

Will the trend toward using biofuels benefit the earth? Listen in as our Chromies team discusses different types of biofuels and how chromatography plays a role in developing mass produced and energy efficient biofuels.

In vaccine development, safety and efficacy are critical. Recent studies have shown that multiplex immunoassays offer a flexible way to monitor immune responses. With the ever-increasing pressure on scientists to develop a practical COVID-19 vaccine, multiplex immunoassays stand out as a proven tool.

The Process Chromatography Team at Bio-Rad offers a variety of resources to address scientists’ needs during the downstream purification process. Visit our robust online library, which contains application notes, tutorials, webinars, and more, or contact us to speak with a specialist or request a customized seminar.

Adeno-associated viruses (AAVs) are some of the most commonly used viral vectors for gene therapy, supporting approximately 250 clinical trials to date. However, the manufacturing processes are challenging to scale. Read about five key issues that gene therapy manufacturers who use AAVs need to look out for.

The Reliance Select cDNA Synthesis Kit is the newest reverse transcription product from Bio-Rad. This high-performance kit features Reliance Reverse Transcriptase, a novel chimeric enzyme that delivers superior results across a broad dynamic range, even with challenging samples. The Reliance Select cDNA Synthesis Kit delivers more accurate reverse transcription quantitative PCR (RT-qPCR) results, even from samples containing PCR inhibitors or degraded RNA.

From quantifying a patient’s tumor DNA to counting the viral load of an individual infected with COVID-19, the breadth of digital PCR (dPCR) applications continues to grow and fulfill critical scientific needs. dPCR offers the ability to count nucleic acid molecules directly, rather than extrapolating the quantity from standard curves as with many other methods. By harmonizing diagnostic procedures on dPCR platforms, the scientific and medical communities can become more coordinated and efficient.