Traci Lyons describes how PrimePCR Assays ended her laboratory’s struggle to find a qPCR assay that could reliably detect transcription of the cyclooxygenase-2 (Cox-2) gene, which they had identified as potentially prometastatic. Using the PrimePCR Assay for Cox-2, researchers in her lab were finally able to verify cyclooxygenase-2 knockdown, which is a necessary step toward confirming its role in breast cancer metastasis.
What’s the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5′ guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.
Follow these six simple tips and you’ll increase the reproducibility of your qPCR experiments.
Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad’s SingleShot Cell Lysis Kit, PreAmp Supermix, and PrimePCR validated primer panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.