
#5 How Do You Choose the Right Hardware for Your Chromatography Application?
Which valves, pumps or detectors are optimal for your FPLC system? How do you achieve optimal gradient performance? Here are some tips.

Optimizing to Automation: The Advantages of Multidimensional (Multi-D) Chromatography
Philip Chapman of Bio-Rad Laboratories discusses the advantages of automated multidimensional (Multi-D) chromatography compared to traditional multistep purification strategies.

Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography
From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don’t have to worry about proteases, we share helpful tips for developing a sample preparation protocol.

Five Tips for Picking the Right Cell Disruption Method for Protein Analysis
Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.

#4 How Do You Listen to Your FPLC UV Detector?
Here are some tips to fix random spikes in your peaks and pulsations in your gradient.

Bio-Rad’s Multi-D Method Addresses Unmet Chromatography Needs
Some of the frustrations in protein purification stem from unmet chromatography needs, such as automation, productivity, throughput, and reproducibility among others. Bio-Rad’s new Multi-D chromatography method addresses these and provides solutions to faster, efficient and more reliable protein purification.

#3 How Do You Care for Your Chromatography System?
Here are some handy tips for maintaining your chromatography columns in great shape.

#2 How Do You Best Prepare Your Columns for Chromatography?
Here are some tips for attaching, filling or running your chromatography columns.

Advantages of Multidimensional (Multi-D) Chromatography Using the NGCâ„¢ Chromatography System over Traditional Sequential Chromatography
Traditional sequential chromatography typically requires intensive hands-on user manipulation, which could hinder the reproducibility of protein purification runs. Multi-D chromatography aims to improve this process while maintaining the efficacy of the traditional workflow.

#1 How Do You Best Prepare Your Protein Samples for Purification?
Here are some tips to clarify your lysate, filter proteins, and to achieve optimal binding of your proteins.