Bioradiations Staff
Minimize On-Column Aggregates during mAb Puriļ¬cation
The Challenge
Have you seen two-peak elution of target monoclonal antibodies (mAb) after cation exchange (CEX) chromatography? The second elution peak consists of a mixture of monomers and aggregates of your target mAb and it leads to a decrease in your process yield.
Puriļ¬cation Tip
Choosing the optimal resin for your process will minimize on-column aggregates and maximize recovery. Be sure to screen multiple resins during your mAb puriļ¬cation process development.
Our Approach
On-column aggregate formation depends on the speciļ¬c chromatography resin used. Resin structure, surface extenders, pore size and distribution each contribute to on-column aggregate formation. Bio-Radās Nuvia HR-S Resin ā a strong cation exchanger with a pore size of 50 Āµm without surface extenders ā minimizes on-column aggregates. Nuvia HR-S generates only 10% aggregate formation relative to competing resins under similar experimental conditions.
See how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification.
Select resins by clicking on the buttons on the left.
Eshmuno S
Capto SP ImpRes
POROS 50 HS
Capto S ImpAct
Fractogel SO3–
Nuvia HR-S
