Electrophoresis-western-blotting

2-D electrophoresis followed by immunodetection of protein spots using western blotting is adopted commonly in proteomics studies. Here we present step-by-step instructions for obtaining accurate overlaps between the gel and the blot using the PDQuest 2-D gel analysis software.

Bio-Rad’s 2-D electrophoresis workflow offers better results in a shorter time. Take a detailed look at this workflow by downloading the updated edition of our How-To Guide.

Bio-Rad’s innovative Gel Doc EZ imaging system features something unique that no other imaging system has – brains.

Learn how scientists in the Gene Regulation Laboratory at the Korea Advanced Institute of Science and Technology (KAIST) use the ChemiDoc MP imaging system in their work investigating the molecular machinery involved in epigenetic gene regulation.

When Dr. Fred Bauzon started his research, he worked with bioinformatics specialists to functionalize suitable gene candidates based on their expression levels with HMGCR transcript levels. All his experiments demonstrated a reduction in low-density lipoprotein receptor protein (LDLR) and candidate gene protein levels after the candidate gene was silenced. Here Bauzon discusses how TGX Stain-free precast gels have aided his research toward future drug targets for the therapeutic control of LDL uptake.

Learn how scientists in the Department of Animal Physiology at the University of Erlangen in Erlangen, Germany use the ChemiDoc MP imaging system in their work investigating sensory systems, in particular, the eye.

Bio-Rad’s new Clarity Western ECL substrate provides high sensitivity, long signal duration, and low background even at long exposures.

Bio-Rad’s latest semi-dry transfer system, the Trans-Blot® Turbo™, has changed the way researchers think about western blot transfers.

Learning to use analysis software can be time-consuming and following a manual can be challenging.

The nine PDQuest tutorials are chapterized so you can focus on what you want to learn. Each video runs from 3 to 9 minutes and features callout boxes that highlight important information to complement the audio explanations.