
Choosing Validated Antibodies for Trustworthy Data
The lack of reproducibility in research has been a source of frustration for scientists. Learn why antibody validation is critical for ensuring specificity and what validation methods Bio-Rad is implementing. Our scientists validate the performance of antibodies through CRISPR-Cas9 gene editing by knockout (KO), siRNA knockdown (KD), and immunoprecipitation followed by mass spectrometry (IP-MS).

Generating Semi-Quantitative Western Blot Data Using Bio-Rad’s Image Lab™ 6.0 Software
Presented by: Kenneth J. Oh, PhD, Applications, Collaborations, and New Technology Manager, Protein Quantitation Marketing Group, Bio-Rad Laboratories, Inc.
Date: Tuesday, March 6, 2018
Western blotting is a popular tool for protein quantitation, and the advent of digital imaging has greatly increased the sensitivity, dynamic range, and quantitation limits of this technique. In the second webinar in this series, get step-to-step instructions for normalizing western blot data using Image Lab 6.0 Software.

Tips to Make Fluorophore Picking Easier
Fluorescence is a property of photo-excitable molecules that has been exploited to develop dyes, or fluorophores, that are used in a variety of experimental procedures, including western blotting. Learn more about the science behind fluorescent dyes and get some helpful tips on how to pick the right fluorophores for your experimental needs.

Identify Major Immune Cell Types by Their CD Marker Expression
Our new immune cell lineage and marker database provides an interactive way to help you easily examine detailed marker data, view immune cell marker expression, and find the antibody to your marker. Learn more and try out the interactive tool for yourself.

Validating Antibodies — the Good, the Bad, and the Necessary
Despite the fact that antibodies are one of the most frequently used tools in life science and clinical research, there are no universally accepted guidelines to ensure that they have been properly validated. Learn about the issues facing researchers and what antibody suppliers and researchers can do to ensure that the research being carried out is trustworthy and is helping to improve overall data quality.

Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol
Western blotting using fluorescently tagged detection antibodies is a technique that is gaining in popularity. However, adopting a new technique can be challenging. Use the tips provided in this article to get started and see how fluorescent western blotting can enhance your scientific research.

The Fluorescent Way: Why Start Fluorescent Western Blotting Now?
Find out why fluorescent western blotting is gaining in popularity and how it compares to more widely used methods, such as chemiluminescence. From easier multiplexing to brighter fluorophores to easier method development, learn about the many advantages of fluorescent western blotting.

TGX Stain-Free™ FastCast™ Acrylamide Gel Casting
This short video demonstrates how you can access the unique benefits of Bio-Rad’s proprietary TGX Stain-Free Gel chemistry by hand casting your own TGX Stain-Free Gels in just a few quick and easy steps.

Meet the Brightest ECL Substrate Around — Clarity Max™ Western ECL Substrate
See how our new high-sensitivity western ECL substrate, Clarity Max, can help you achieve greater western blotting success.

Six Tips for Efficient Transfer of Your Proteins
A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.