From the origins of digital PCR in leukemia research to stopping HIV with novel polymers to better methods for fragment-based drug discovery, BioRadiations has covered some of the most exciting life science research stories of 2013. Now take a look back at these stories and more as we review the best pieces of the year.
Twenty years after the discovery of the link between BRCA gene mutations and breast cancer, researcher Ryan Jensen of the Yale Medical School is looking into the interactions between the DNA repair protein RAD51 and BRCA proteins using Bio-Rad’s V3 Western Workflow.
Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting
2-D electrophoresis followed by immunodetection of protein spots using western blotting is adopted commonly in proteomics studies. Here we present step-by-step instructions for obtaining accurate overlaps between the gel and the blot using the PDQuest 2-D gel analysis software.
Bio-Rad’s 2-D electrophoresis workflow offers better results in a shorter time. Take a detailed look at this workflow by downloading the updated edition of our How-To Guide.
Image Lab™ 4.1, the Latest Software Version, Addresses the Challenges of Multiplex Analysis and Protein Normalization
The updated Image Lab image acquisition and analysis software provides improved tools for total protein normalization using the stain-free technology.
Although the Western blot is a core lab procedure, surprisingly little has changed in traditional bench work practice since its inception in 1979. The V3 Western Workflow delivers a streamlined solution to the conventional western blotting workflow as explored here by one protein researcher specializing in chronic obstructive pulmonary disease (COPD).
Bio-Rad has long demonstrated innovation in imaging, with the introduction of the stain-free gel electrophoresis workflow, which was rapidly followed by the launch of the Gel Doc™ EZ imager. Now, this progression continues with the release of the ChemiDoc™ MP imaging system.
Mini-PROTEAN® TGX Stain-Free™ and Criterion™ TGX Stain-Free™ precast gels, when combined with Bio-Rad’s ChemiDoc™ MP or Gel Doc™ EZ imagers, provide researchers with the fastest protein separation and visualization available using the standard Laemmli system.
The ChemiDoc MP imaging system enables stain-free operation and lets you visualize proteins at every stage of your experiment. Its flexibility and sensitivity are complemented by a simple, intuitive operation that integrates seamlessly into your workflow.