This presentation by Dr. Dawne Shelton describes how Droplet Digital PCR (ddPCR™) can be used to measure circulating nucleic acids (cfDNA) and circulating tumor cells (CTCs) in blood and to detect rare tumorigenic mutations down to 0.01% or less in a high background of normal DNA. Techniques and methods discussed in this webinar will enable you to begin your own investigations.
Droplet Digital PCR (ddPCR™) has revolutionized infectious disease research, cancer biomarker and genomic variation analysis, and other fields. This webinar by Dr. George Karlin-Neumann provides an overview of the ddPCR concept and workflow and discusses recent journal publications showcasing the technology’s diverse applications.
In the first half of this two-part presentation, Dr. Anton Posch, an expert in 1- and 2-D electrophoresis, provides tips and tricks for the 2DE workflow, covering sample preparation, protein extraction and solubilization, reduction and alkylation of protein side chains, and IEF. The webinar highlights critical success factors and is aimed at both novices and experts wanting to sharpen their skills and keep up on new advances in the field.
In the second part of this presentation, Dr. Posch details IEF separation, use of immobilized pH gradient (IPG) strips, running second-dimension PAGE, spot detection, gel matching, and data analysis. The Q&A elaborates on sample preparation, storage, and running conditions.
In this webinar, Prof. Alexander Urban of the Stanford School of Medicine describes how his lab used the QX200™ Droplet Digital™ PCR System to investigate copy number variation (CNV) in induced pluripotent stem cells (iPSCs) that were being used to study the genetics of autism.
This presentation by Vicki Hwang details how UC Davis researchers used Droplet Digital PCR (ddPCR™) technology to determine copy number variation (CNV) and characterize the deletion endpoints of the region of chromosome 22 that is deleted in 22q11 deletion syndrome.
In this webinar, Dr. David Dodd describes how he and Prof. David Corey and colleagues at UT Southwestern Medical Center have used Droplet Digital™ PCR (ddPCR™) in their research into low-abundance targets with therapeutic potential for cancer and Huntington’s disease.