
As Easy as 1, 2, 3: Achieving Purification Success with a New Two-Mode Chromatography Resin
A common misconception is that stronger is always better. However, Nuvia wPrime 2A Media, a weak anion exchange hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin, has won over purification scientists. Read about diverse use cases for Nuvia wPrime 2A Media and see the data for yourself.

Development of an IEX Purification Process for Lentiviral Vectors
In recent years, gene therapy treatments have increased significantly, requiring more efficient processes to purify and deliver these therapeutics. Lentiviral vectors (LVVs) are a type of retrovirus and are an effective tool for gene delivery. In this webinar, data are shown for a high-throughput screening strategy that was employed for LVV purification, which provided high yield as well as acceptable host cell protein (HCP) clearance.

Strategies to Address Large Molecule Purification Challenges
Large molecule purification presents significant challenges due to their considerable size. For instance, Immunoglobulin M (IgM) molecules, ranging from 900 to 1,000 kD, play pivotal roles in mammalian immune responses and find extensive applications in diagnostics and therapeutics. Purification of such molecules is particularly intricate owing to several factors including their weak Protein A binding sites, limited dynamic binding capacity, and slow diffusion rates.

Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography
Anion exchange and mixed-mode chromatography offer efficient and flexible AAV purification. Learn new strategies for optimizing viral vector purification.

Single-Step Influenza Virus Purification Using an Anion Exchange Resin
A new and simplified anion exchange chromatographic process for the purification of both live and inactivated cell grown H1N1 influenza viruses was established with a recently developed anion exchanger. The resin is designed with an optimized surface extender and pore size for superior accessibility and large biomolecule binding capacity, aiming to overcome common drawbacks of existing ion exchangers for virus purification.

Single-Step Column Purification for H1N1 Virus
Using Nuvia HP-Q AEX Resin
Learn how a team developed a single-step workflow for column purification of the Influenza A H1N1 subtype using the recently developed Nuvia HP-Q Anion Exchange Resin. This protocol is scalable, efficient, and produced good yields with high purity.

Serving your Downstream Purification Needs
The Process Chromatography Team at Bio-Rad offers a variety of resources to address scientists’ needs during the downstream purification process. Visit our robust online library, which contains application notes, tutorials, webinars, and more, or contact us to speak with a specialist or request a customized seminar.

Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media
See how you can use CHT XT Mixed-Mode Media to recover over 75% of your virus in a single purification step. All this while reducing contaminating proteins by over 90% and removing over 60% of dsDNA. Get simple, fast, and reproducible viral purification with CHT XT.

A Resin Built Specifically for Large Biomolecule Purifications
Nuvia HP-Q is a high-performance strong anion exchange resin designed for purification of large biomolecules. See how its optimized particle and pore size leads to high target recovery and high-throughput purifications.