Choosing Validated Antibodies for Trustworthy Data
The lack of reproducibility in research has been a source of frustration for scientists. Learn why antibody validation is critical for ensuring specificity and what validation methods Bio-Rad is implementing. Our scientists validate the performance of antibodies through CRISPR-Cas9 gene editing by knockout (KO), siRNA knockdown (KD), and immunoprecipitation followed by mass spectrometry (IP-MS).
A Scalable Immobilized Metal Affinity Chromatography Media for Process Purification
Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity (DBC) that provides production efficiencies and good process economics. Nuvia IMAC Resin provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operational run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure. Protein purification can efficiently be scaled up from milligrams in the lab to kilograms in bioprocess manufacturing. The chemical stability of Nuvia IMAC Resins ensure efficient column regeneration, reproducibility between purification runs, extended column lifetime, and product purity. Watch our video to learn how Nuvia IMAC Resins overcome the challenges associated with process purification of proteins using metal affinity chromatography.
Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography
Purifying proteins that are not highly expressed can be challenging. Watch this video to learn about a case study demonstrating an efficient workflow for two-step purification of a low-expressing recombinant protein using mixed-mode chromatography.
Single-Step Column Purification for H1N1 Virus
Using Nuvia HP-Q AEX Resin
Learn how a team developed a single-step workflow for column purification of the Influenza A H1N1 subtype using the recently developed Nuvia HP-Q Anion Exchange Resin. This protocol is scalable, efficient, and produced good yields with high purity.
Advantages of Multiplexing with Bio-Plex Assays
The Bio-Plex Assay development process is designed to produce high performance multiplex assays. We prioritize sensitivity, broad dynamic range, reproducibility, and shorter incubation times to get you meaningful results faster. Watch this video to learn more about our SMARTS approach to assay development.
Discover lncRNA: Understanding the Long Noncoding Transcriptome
Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).
Identifying Predictive Biomarkers for Successful Immunotherapy Treatments
Find out how the S3e™ Cell Sorter can help scientists discover new biomarkers that have the potential to become new therapies for cancer and other diseases.
Top Ten Videos of 2017
Explore the most watched Bio-Rad videos in 2017. From understanding CRISPR gene editing to generating better qPCR data to selecting antibodies for fluorescent western blotting, these videos highlight how we continually strive to help our customers do better science.
Get More Answers from Your Mouse with the Bio-Plex Pro™ Mouse Chemokine Panel
Biomarker screening can sometimes be like working in a black box. While traditional research tools focus on one protein at a time, Bio-Rad’s new Bio-Plex Pro™ Mouse Chemokine Panel can simultaneously interrogate 33 chemokines and cytokines in a single well. See how you can get more answers with this panel.
TGX Stain-Free™ FastCast™ Acrylamide Gel Casting
This short video demonstrates how you can access the unique benefits of Bio-Rad’s proprietary TGX Stain-Free Gel chemistry by hand casting your own TGX Stain-Free Gels in just a few quick and easy steps.