protein purification

Monoclonal antibodies (mAbs) increasingly comprise a large portion of the product pipeline of major biopharmaceutical companies. Challenges in mAb purification include reducing production cost, developing robust processes for both product purity and viral clearance, and integrating upstream …

Traditional Protein A affinity chromatography is the gold standard for Fc-fusion protein purification, but protein denaturation and degradation due to low-pH elution are limitations. This webcast presents a proof-of-concept study demonstrating the use of …

A common misconception is that stronger is always better. However, Nuvia wPrime 2A Media, a weak anion exchange hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin, has won over purification scientists. Read about diverse use cases for Nuvia wPrime 2A Media and see the data for yourself.

Learn how Bio-Rad’s Nuvia wPrime 2A Media, a weak AEX-HIC resin, is used in an efficient monoclonal antibody (mAb) purification process. Presented by Dr. Sushmita Koley, this webcast demonstrates how multimodal chromatography improves purity and recovery, achieving a 10x reduction in high molecular weight species while maintaining 85% mAb monomer recovery. Watch to explore innovative solutions for biomanufacturing.

Nuvia wPrime 2A Resin has MMAEX functionality with a weak anion exchanger. See data showing improved mAb and bsAb purification compared to traditional resins.

Choosing the right chromatography resin is a complex and critical decision that can significantly impact the success of your downstream purification workflow, whether for preparative or analytical applications. To make an informed choice, you must consider the scientific principles of the process, practical implications for your specific application, and attributes of the manufacturer.

Follow the story of Evitria as it grows from a small Chinese hamster ovary (CHO) cell supernatant supplier to a global therapeutic antibody production company serving clients across the globe. Bio-Rad has partnered with Evitria at every stage of this remarkable journey and looks forward to supporting its continued success.

Modern chromatographic resins enable new strategies for downstream processing of bispecific antibodies. Watch this webinar to see case studies and workflows.

Large molecule purification presents significant challenges due to their considerable size. For instance, Immunoglobulin M (IgM) molecules, ranging from 900 to 1,000 kD, play pivotal roles in mammalian immune responses and find extensive applications in diagnostics and therapeutics. Purification of such molecules is particularly intricate owing to several factors including their weak Protein A binding sites, limited dynamic binding capacity, and slow diffusion rates.

For over 30 years, CHT Ceramic Hydroxyapatite Chromatography Media has been a trusted, proven purification solution for many biopharmaceutical manufacturing processes. CHT Media provides exceptional removal of impurities when other chromatography resins fall short. In this webcast, explore the essential facts and benefits of this chromatography technology.