A Gentler Workflow: Fc-Fusion Protein Purification with Mixed-Mode and Anion Exchange Chromatography
Traditional Protein A affinity chromatography is the gold standard for Fc-fusion protein purification, but protein denaturation and degradation due to low-pH elution are limitations. This webcast presents a proof-of-concept study demonstrating the use of …
Manufacturing a Recombinant Retrovirus — Downstream Purification Process Development
Enveloped virus and VLP purification can be difficult. Learn about key factors and a cGMP-compatible, low cost, scalable process for retrovirus vector capture.
The Process of Resin Screening
Process development scientists, faced with an increasing number of proteins or complex biomolecules, continuously search for robust purification methods that can be rapidly scaled for manufacturing. What are the optimal purification conditions for high throughput and yield? Which resin or resin combination best purifies the desired protein? How can an efficient, yet cost-effective process be established? The answer: use a systematic screening approach. Read on to learn more.
Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography
Purifying proteins that are not highly expressed can be challenging. Watch this video to learn about a case study demonstrating an efficient workflow for two-step purification of a low-expressing recombinant protein using mixed-mode chromatography.
Serving your Downstream Purification Needs
The Process Chromatography Team at Bio-Rad offers a variety of resources to address scientists’ needs during the downstream purification process. Visit our robust online library, which contains application notes, tutorials, webinars, and more, or contact us to speak with a specialist or request a customized seminar.