What a Single Cell Can Tell You: Exploring Gene Expression and Regulation of Single Cells
RNA sequencing (RNA-Seq) is a fundamental technique for transcriptome profiling, facilitating the detection of gene expression changes across sample groups. Traditional RNA-Seq of complex tissues, however, is not capable of deciphering the unique biological processes that take place within each cell. As such, single-cell RNA-Seq (scRNA-Seq) has emerged for the analysis of RNA molecules from individual cells, uncovering detailed insights that are unobtainable through bulk RNA-Seq.
Sequenced — Comic Series
Join Terence, a motivated undergrad, as he experiences the ups and downs of research using next-generation sequencing, learning valuable tips for successful NGS experiments along the way
Bridging the Gap: Using SEQuoia RNA-Seq Library Prep Kits with New Short-Read Sequencing Platforms
RNA sequencing (RNA-Seq) is a fundamental discovery technique for determining the expression patterns of encoded genes. RNA-Seq has led to innumerable breakthroughs across disciplines; however, a need remains for new sequencing and library preparation technologies to address gaps in current methods. Read how improved access to RNA-Seq would benefit translational research and how a novel RNA library preparation chemistry can synergize with new sequencing platforms.
6 Key Considerations for Getting the Long and Short of RNA-Seq with FFPE Samples
When it comes to tumor profiling, the poor quality and degradation of RNA in formalin-fixed paraffin-embedded (FFPE) samples are major limitations. To overcome this challenge, we tested a novel RNA-Seq workflow using the SEQuoia Complete Library Prep Kit and the SEQuoia Ribodepletion Kit. This workflow offers more complex transcriptome profiling, including both long and short RNA biotypes, to better represent the complete transcriptome.
Take the Expressway to RNA Library Prep
RNA-Seq library preparation is a manually intensive, time-consuming process prone to bias from the multiple enzymes required. So, how can you trust your sequencing results? Bio-Rad is revolutionizing RNA-Seq library prep with the SEQuoia Express Stranded RNA Library Prep Kit – a three-hour, three-tube solution eliminating the need for multiple enzymes and reducing both the number of pipetting steps and protocol time. The key is an engineered, proprietary retrotransposon called SEQzyme. With this serial template jumping, or continuous synthesis, reaction you can obtain an unbiased, stranded RNA-Seq library constructed to accurately represent both messenger and non-coding RNA from your sample.