How to Design Primers for qPCR
How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.
Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography
From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don’t have to worry about proteases, we share helpful tips for developing a sample preparation protocol.
Five Tips for Picking the Right Cell Disruption Method for Protein Analysis
Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.
Four Tips for Handcasting SDS-PAGE Gels
Watch this episode of LabNotes for helpful tips like how to pour the stacking and resolving gel at the same time.
Four Tips for Running the Perfect SDS-PAGE Gel
We share five simple tricks that address common gel running woes, from samples that float out of wells to sample buffers that turn yellow.
Choosing the Right Percentage Gel
Unsure about what percentage SDS-PAGE gel to pick for your experiment? We share four tips that make picking the perfect gel a piece of cake.
Automated Multi-D Chromatography on the NGCâ„¢ System
What is Multidimensional (Multi-D) chromatography? Here is an animated explanation of how Multi-D chromatography works and what its advantages are over traditional chromatography.
Minimizing Protein Degradation
In this episode, we share tips and tricks for minimizing protein degradation during cell lysis and sample preparation.
High Quality Gene Expression from Precious Samples
— A Simplified Workflow
Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad’s SingleShot Cell Lysis Kit, PreAmp Supermix, and PrimePCR validated primer panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.
Teaching Inquiry in the Classroom
A look inside Julianne Zedalis’ classroom at The Bishop’s School in San Diego, CA, gives a glimpse of how a master teacher implements inquiry in her classroom. Bio-Rad’s ThINQ! Investigations enable instructors to guide students through real-world inquiry investigations. These labs meet the revised College Board curriculum requirements and are aligned to AP Biology Big Ideas; they are designed to connect inquiry to NGSS and college curricula, encouraging students to think like real scientists.