Monoclonal Antibody Purification: A Two-Step Approach Using a Strong AEX-HIC Mixed-Mode Resin
Monoclonal antibodies (mAbs) increasingly comprise a large portion of the product pipeline of major biopharmaceutical companies. Challenges in mAb purification include reducing production cost, developing robust processes for both product purity and viral clearance, and integrating upstream …
A Gentler Workflow: Fc-Fusion Protein Purification with Mixed-Mode and Anion Exchange Chromatography
Traditional Protein A affinity chromatography is the gold standard for Fc-fusion protein purification, but protein denaturation and degradation due to low-pH elution are limitations. This webcast presents a proof-of-concept study demonstrating the use of …
Evaluation of A New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges
Multimodal chromatography has a history of providing value for biopharmaceutical separations. Ligands that enable binding based on both charge and hydrophobic interactions provide more opportunity for effective separation of multiple …
As Easy as 1, 2, 3: Achieving Purification Success with a New Two-Mode Chromatography Resin
A common misconception is that stronger is always better. However, Nuvia wPrime 2A Media, a weak anion exchange hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin, has won over purification scientists. Read about diverse use cases for Nuvia wPrime 2A Media and see the data for yourself.
Demonstrating a Modular Selectivity with a Weak AEX-HIC Resin for Achieving High mAb Purity and Recovery in a Downstream Process
Learn how Bio-Rad’s Nuvia wPrime 2A Media, a weak AEX-HIC resin, is used in an efficient monoclonal antibody (mAb) purification process. Presented by Dr. Sushmita Koley, this webcast demonstrates how multimodal chromatography improves purity and recovery, achieving a 10x reduction in high molecular weight species while maintaining 85% mAb monomer recovery. Watch to explore innovative solutions for biomanufacturing.
Evaluation of a New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges
Nuvia wPrime 2A Resin has MMAEX functionality with a weak anion exchanger. See data showing improved mAb and bsAb purification compared to traditional resins.
Enhancing Biotherapeutic Purification Workflows with Mixed-Mode Chromatography
As biotherapeutic modalities have diversified and production processes become more advanced, an evolution in purification strategies is also needed. Modern biotherapeutic purification must be capable of removing multiple types of impurities without compromising yield or quality, in a cost-effective manner. To meet these needs, many are turning to mixed-mode chromatography.
CHT Chromatography Media: 5 Essential Facts
For over 30 years, CHT Ceramic Hydroxyapatite Chromatography Media has been a trusted, proven purification solution for many biopharmaceutical manufacturing processes. CHT Media provides exceptional removal of impurities when other chromatography resins fall short.
In this webcast, explore the essential facts and benefits of this chromatography technology.
A Polishing Strategy for Removing Impurities in Bispecific Antibody Purification
Bispecific antibodies pose unique challenges in downstream processing. See how impurities were efficiently removed by mixed-mode chromatography in this webinar.
Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography
Anion exchange and mixed-mode chromatography offer efficient and flexible AAV purification. Learn new strategies for optimizing viral vector purification.