ArticlesReal-time qPCR/PCRTechnical Reports

PrimePCR™ Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation

The MIQE guidelines have set a new standard for publishing qPCR results to ensure integrity in the scientific literature and increase experimental transparency. One of the key guidelines focuses on the proper design and experimental validation of primer assays used in qPCR. This tech report describes the design and wet-lab validation of PrimePCR assays and how they comply with the MIQE guidelines.
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ArticlesReal-time qPCR/PCRTechnical Reports

A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data

This tech report shows how qPCR can lead to erroneous conclusions regarding differences in MCM7 gene expression between normal and tumor human breast cancer samples if the key steps set out in the MIQE guidelines are not followed.
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