As Easy as 1, 2, 3: Achieving Purification Success with a New Two-Mode Chromatography Resin
A common misconception is that stronger is always better. However, Nuvia wPrime 2A Media, a weak anion exchange hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin, has won over purification scientists. Read about diverse use cases for Nuvia wPrime 2A Media and see the data for yourself.
Demonstrating a Modular Selectivity with a Weak AEX-HIC Resin for Achieving High mAb Purity and Recovery in a Downstream Process
Learn how Bio-Rad’s Nuvia wPrime 2A Media, a weak AEX-HIC resin, is used in an efficient monoclonal antibody (mAb) purification process. Presented by Dr. Sushmita Koley, this webcast demonstrates how multimodal chromatography improves purity and recovery, achieving a 10x reduction in high molecular weight species while maintaining 85% mAb monomer recovery. Watch to explore innovative solutions for biomanufacturing.
Evaluation of a New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges
Nuvia wPrime 2A Resin has MMAEX functionality with a weak anion exchanger. See data showing improved mAb and bsAb purification compared to traditional resins.
Strategies to Address Large Molecule Purification Challenges
Large molecule purification presents significant challenges due to their considerable size. For instance, Immunoglobulin M (IgM) molecules, ranging from 900 to 1,000 kD, play pivotal roles in mammalian immune responses and find extensive applications in diagnostics and therapeutics. Purification of such molecules is particularly intricate owing to several factors including their weak Protein A binding sites, limited dynamic binding capacity, and slow diffusion rates.
Nature’s Lipid Nanoparticles: Challenges and Opportunities in Isolation and Analysis of Extracellular Vesicles
In nature, mammalian extracellular vesicles (EVs) are vital for intercellular communication, immune responses, and cell removal. EVs also hold promise in diagnostics, therapeutics, and vaccine delivery due to their abundance and natural role as carriers. Explore how overcoming challenges in EV purification and precise RNA analysis methods can help unlock their potential applications.
Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography
Anion exchange and mixed-mode chromatography offer efficient and flexible AAV purification. Learn new strategies for optimizing viral vector purification.
Efficient and Rapid Purification of E. coli–Expressed Toxin Recombinant Protein Fragments
Tetanus toxoid (TT) is a common carrier protein for conjugate vaccines, but its production is faced with significant challenges. Learn about a purification process that yields over 98% pure target protein in this video.
Assessing Viral Clearance in Early-Phase Process Development
Viral clearance studies are part of a multifaceted approach to ensure biopharmaceutical product safety. To prevent costly changes to a manufacturing process, it is important to assess each operation unit for its efficiency early on during downstream process development. See how a design of experiments (DOE) approach was utilized in this case study to investigate the effect of buffer pH and conductivity on the removal of impurities by an anion exchange (AEX) mixed-mode chromatography resin.
Purification of DNA Oligonucleotides using Anion Exchange Chromatography
In this webcast, see how Nuvia HP-Q Resin, an anion exchange resin, was used to purify oligonucleotides with phosphodiester backbones in a scaleable workflow.
Large Biomolecule Purification with a New High-Performance Anion Exchange Resin
Presented by: Hana Kim, PhD, Global Product Manager, Process Chromatography
Jiali Liao, PhD, Principal Scientist, Process Chromatography
View on demand
Learn about Nuvia HP-Q Resin, a new rigid bead engineered with large pores and optimized surface extenders to deliver both high binding capacity and high recovery at high flow rates. It is ideal for large molecule purification, including plasma proteins, viruses, and VLPs.