High Quality Gene Expression from Precious Samples
— A Simplified Workflow
Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad’s SingleShot Cell Lysis Kit, PreAmp Supermix, and PrimePCR validated primer panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.
Teaching Inquiry in the Classroom
A look inside Julianne Zedalis’ classroom at The Bishop’s School in San Diego, CA, gives a glimpse of how a master teacher implements inquiry in her classroom. Bio-Rad’s ThINQ! Investigations enable instructors to guide students through real-world inquiry investigations. These labs meet the revised College Board curriculum requirements and are aligned to AP Biology Big Ideas; they are designed to connect inquiry to NGSS and college curricula, encouraging students to think like real scientists.
Cell Imaging Simplified – the ZOE™ Fluorescent Cell Imager
The ZOE fluorescent cell imager features an integrated digital camera and touch-screen interface that makes brightfield and multichannel fluorescence microscopy simple, fast, and intuitive. An easy-to-use alternative to the traditional inverted microscope, ZOE eliminates the complexities of multichannel fluorescence imaging by combining the ease of use of a personal tablet and the power of an inverted microscope.
Tips for Keeping Your NGC™ Chromatography System in Great Shape
Doing these three simple checks will protect your chromatography system from common lab hazards.
Tips for Using Chromatography Columns with Your NGC™ Chromatography System
Here are the methods you need to ensure optimal, long-lasting column performance.
Tips for Sample Preparation with Your NGC™ Chromatography System
Follow these steps to go from crude cell lysate to a sample that’s ready to purify.
Tips for Storing Proteins Purified on Your NGC™ Chromatography System
Get the most out of your proteins, including better stability, the best ways to freeze fractions, and more!
Tips for Preparing and Using Buffers with Your NGC™ Chromatography System
Here are four fast and easy things that will help you maintain peak performance for your medium pressure chromatography system. Beat contamination and save your samples!
PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells – A Fast Approach to Staining Nuclei
PureBlu Hoechst 33342 Nuclear Staining Dye is the optimal choice for staining the nuclei of live cells for ultraviolet imaging. This short video goes through the steps involved in staining live cells with PureBlu Hoechst 33342 Nuclear Staining Dye.
PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate
One easy way to obtain a PrimePCR plate customized for your research is to modify one of Bio-Rad’s predesigned panels. This short video tutorial shows you how to use the online tools at Bio-Rad.com to edit a predesigned plate and create, then order your own customized 96- or 384-well PCR plate.