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See NEUE Documentation, 10. Shortcodes:
Know your transfer method
Understanding the different methods of transfer and which one would be optimal for your protein is the first step. Wet transfer, where the membrane sandwich is completely immersed in the transfer buffer in a buffer tank, is used for transferring proteins of broad molecular weights. In the semi-dry method of transfer, the buffer is restricted to the two stacks of filter paper covering the membrane. Semi-dry conditions are favorable for transferring proteins in the 30–120 kD range. A third method, the rapid semi-dry transfer has special buffer and material formulations that facilitate the transfer of proteins in as short a time as in 3–10 minutes. The rapid semi-dry method can be used for proteins with a broad range of molecular weights.
Note: This is a screenshot of the shortcode (below). Use the Column shortcode under the [SC] menu. Protocols and Tips number blocks use a combination of 1/4 + 3/4 column.