Research Highlights | Bio-Radiations

Gene Editing: Navigating the Path from Innovation to Application

Bioradiations Staff — Mon, 27 Apr 2026 20:16:19 +0000

Gene editing is rapidly evolving, and its relevance across biotechnology and medicine continues to expand. As these tools advance, so does the need for reliable ways to measure editing efficiency ...

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The Journey from Lab to Clinic: Zolgensma, the First Gene Therapy Treatment for Spinal Muscular Atrophy (SMA)

Bioradiations Staff — Tue, 11 Feb 2025 20:16:49 +0000

Translational research bridges the gap between scientific discoveries and real-world applications, transforming scientific breakthroughs into life-changing therapies. Success in this endeavor hinges on several factors, including collaboration between scientists and clinicians. A notable example of this is the approval of Zolgensma, a gene therapy for spinal muscular atrophy (SMA), which marked a significant milestone in the field of translational research.

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CAR T-Cell Therapy and the Cytokine Storm: The Balancing Act at the Center of Next-Generation Cancer Therapies

Bioradiations Staff — Mon, 12 Feb 2024 18:18:07 +0000

Recent advances in chimeric antigen receptor (CAR) T-cell therapy have opened exciting new avenues for the treatment of a variety of malignancies. Cytokine release syndrome (CRS), a powerful immune response to activated CAR T cells, is the primary side effect of CAR T-cell therapy, but existing CRS treatments can make CAR T-cell treatment less effective. In two recent studies, researchers used multiplexed cytokine immunoassays to better understand how CAR T-cell therapy triggers CRS and to identify strategies for early intervention in CRS development.

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Efficacy of a Novel Treatment for Multiple Myeloma Established by Flow Cytometry

Bioradiations Staff — Tue, 14 Nov 2023 19:39:59 +0000

Autologous stem cell transplantation (ASCT) is an effective treatment strategy for multiple myeloma (MM) but isolating enough hematopoietic stem and progenitor cells (HSPCs) for transplantation can be challenging. The selective CXCR4 inhibitor motixafortide has been shown to be a safe and efficacious HSPC mobilization agent in a phase 2 trial when paired with G-CSF. Crees et al. (2023) report the results of the phase 3 trial, in which they used the ZE5 Cell Analyzer to explore motixafortide’s mobilizing effects.

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Advancing Molecular Diagnostics for Myelodysplastic Syndrome

Bioradiations Staff — Wed, 09 Jun 2021 01:03:30 +0000

No patient who has survived cancer wants to hear that they’ve relapsed. But hearing it sooner rather than later can dramatically increase the chances of finding a successful, potentially life-saving treatment. Using next-generation sequencing and Droplet Digital PCR, researchers at the University of Uppsala aim to detect relapse by monitoring patient-specific biomarkers. Read about their ongoing clinical study.

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Immune Surveillance in SARS-CoV-2 Vaccine Development

Bioradiations Staff — Mon, 08 Mar 2021 22:14:23 +0000

The ability to characterize the target of cell-mediated immune response is crucial during vaccine development. In studying the characterization of SARS-CoV-2 infection pathogenesis in humans, a research team identified neutralizing antibodies that provided context for vaccine antigen design. Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein–specific antibodies in a rapid, high-throughput manner.

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How FACS, Genetic Screens, and the HAP1 Cell Line Uncover the Biological Mechanisms of Cancer

Bioradiations Staff — Mon, 08 Mar 2021 20:17:49 +0000

Genetic screens are powerful tools for finding genes associated with a particular phenotype. They play an especially important role in cancer research and are leveraged to identify predictive biomarkers that can be used to improve treatment efficacy or trace the progression of genetic alterations that ultimately lead to disease. The HAP1 cancer cell line model and fluorescence-activated cell sorting (FACS) bring efficiency to the development of genetic screens that is making them a force to be reckoned with.

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Inflamed in the Brain: A Protocol for Studying Neuroinflammation in Mouse Brain Tissue

Bioradiations Staff — Tue, 08 Dec 2020 18:51:33 +0000

Mice are critical model organisms for the study of neuroinflammation. But tissue quantity is often a limiting factor. In a recent paper, researchers describe a protocol for analysis of 33 cytokines and chemokines using only 50 μl of tissue homogenate. The remaining homogenate can be used for RNA extraction or other analysis.

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Sorting an Old Problem: Are Cells Stressed by Fluorescence-Activated Cell Sorting?

Bioradiations Staff — Wed, 09 Sep 2020 21:16:22 +0000

Fluorescence-activated cell sorting is a valuable tool for research, but could the physical forces of sorting stress the cells, altering their behavior and confounding experimental results? Recent research found that although cell sorting activated p38 MAPK stress signaling, no functional or structural changes were observable. The results indicate that jet-in-air systems such as the Bio-Rad S3e Cell Sorter do not alter cell behavior.

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Monocytes — a Mini Review

Bioradiations Staff — Thu, 15 Sep 2016 23:18:26 +0000

Monocytes play key roles in mediating innate and adaptive immune responses. Here is an overview of the types, functions, process of recruitment, and the role of monocytes in disease.

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Macrophage Polarization — An Overview

Bioradiations Staff — Fri, 15 Jul 2016 21:28:12 +0000

Macrophages are important players in the phagocytosis process in the immune system. In this detailed review, we provide an overview on the different types of macrophages, the signaling molecules involved in the polarization of M1 and M2 macrophage subsets, and more.

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So, How Can the Sso7d Fusion Polymerase Technology Help Your PCR?

Bioradiations Staff — Tue, 08 Dec 2015 19:10:10 +0000

During a PCR reaction, it is imperative for the DNA polymerase to remain attached to the template while extending the new strand. This association is jeopardized under challenging PCR conditions and when amplifying longer DNA fragments. See how Sso7d, a small protein capable of binding with the polymerase, helps in stabilizing this association and increasing PCR efficiency.

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Next Generation Computers: Transforming Cells into Autonomous Computing Devices

Bioradiations Staff — Tue, 14 Jul 2015 23:13:50 +0000

What do computers and cells have in common? Can cells function as computers or recording devices? Can a bacterial computer be built? Synthetic biologists have recently developed a platform called SCRIBE (Synthetic Cellular Recorders Integrating Biological Events) to convert genomic DNA into a “tape” for recording and memorizing information. This article explores many interesting aspects of autonomous cellular memory and the potential applications of such living computational systems.

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Researcher Profile: Suzanne Topalian Pioneers PD-1 Inhibitor Drug to Treat Advanced Cancer

Bioradiations Staff — Wed, 27 May 2015 18:01:57 +0000

Suzanne Topalian was recognized in 2014 by Nature as one of “Ten People Who Mattered This Year” for her contributions in pioneering nivolumab, a programmed cell death protein-1 (PD-1) inhibitor that was recently approved in Japan for advanced melanoma treatment and that shifts the paradigm for a wide range of cancer therapies.

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Ebola: New Techniques for Diagnosis, Treatment, and Prevention

Bioradiations Staff — Tue, 09 Dec 2014 18:23:35 +0000

The 2014 West African Ebola outbreak has been an unprecedented public health emergency, with thousands infected across multiple countries and cases spreading internationally. This article examines the science behind this deadly hemorrhagic fever, including its pathogenesis, detection, the challenges it presents in the field, and research into new therapies such as monoclonal antibodies.

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Trends in Protein Separation and Analysis — the Advance of Stain-Free Technology

Bioradiations Staff — Tue, 09 Sep 2014 23:45:23 +0000

What is stain-free technology? How does it work in electrophoresis and western blotting? How does it compare with existing staining techniques? What are its advantages and possible concerns? Here is a technical review of this rapidly growing technological advance.

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Purification Trends: How Smart is Chromatography Automation?

Bioradiations Staff — Wed, 09 Jul 2014 04:56:59 +0000

What does automated protein purification mean today? What might it look like tomorrow? Bioradiations talks to a variety of users about what they’re doing now to implement automation in their purification processes, and what possibilities they envision that will improve chromatography in the near future.

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The Ever-Expanding Universe of Droplet Digital™ PCR Applications

Bioradiations Staff — Tue, 11 Mar 2014 21:24:19 +0000

Bio-Rad’s groundbreaking Droplet Digital^™ PCR technology can be applied in a variety of applications to glean precious information that cannot be obtained by any other method. Find out more about how this technology has been used in applications such as quantification of cancer biomarkers, validation of next generation sequencing, distinguishing genomic variation, and more.

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Building New Anti-HIV Molecules with Bio-Rad’s ProteOn™ XPR36 System

Bioradiations Staff — Wed, 13 Nov 2013 00:47:47 +0000

Can we construct molecules that will interfere with disease pathways? Dan Mitchell of the University of Warwick and his colleagues wanted to apply new synthesis techniques to interrupting HIV infection. Find out what they learned about the potential of these novel molecules using Bio-Rad’s ProteOn™ XPR36 System.

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Digital PCR at the Next Level: Users Find Many Applications for the QX100™ Droplet Digital™ PCR System

Bioradiations Staff — Tue, 12 Mar 2013 19:00:55 +0000

The QX100 Droplet Digital PCR system gives researchers an improvement in PCR precision and sensitivity. See how this innovative digital PCR system is enabling companies to create better products, improve workflow efficiencies, and validate results with greater accuracy than previous methods allowed.

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Stellan Hjertén Wins the California Separations Science Society Award

Bioradiations Staff — Fri, 21 Oct 2011 23:52:25 +0000

This year the California Separation Science Society will present its annual Scientific Achievement Award to Professor Stellan Hjertén of Uppsala University. The award dinner will be held November 3, 2011 at the Waterfront Hotel in Jack London Square.

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Targeting Suppression of B-Cell Lymphoma Proliferation

Bioradiations Staff — Wed, 15 Jun 2011 19:00:29 +0000

Rossi considers the discovery of RNAi as pivotal to antiviral discovery. "RNAi has become another important, powerful genetic tool we can use to further our antiviral work," he says. Rossi's lab became the first to publish research demonstrating the expression of small interfering RNAs (siRNAs) to inhibit HIV replication in human cells. Various aspects of RNAi and siRNAs continue to play a key role in his research on viral diseases and lymphomas.

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Protein Quantitation Assays — Frequently Asked Questions

Bioradiations Staff — Mon, 13 Jun 2011 18:23:22 +0000

Protein quantitation is an important part of many workflows. The estimation or quantitation of the protein in a sample frequently allows researchers to understand the results from their work and make decisions on the subsequent steps. Presented are answers to some of the most frequently ask questions related to usage of Bio-Rad's colorimetric assays for protein quantitation.

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Research Applications for the EpiQ™ Chromatin Analysis Kit

Bioradiations Staff — Wed, 08 Jun 2011 19:00:31 +0000

EpiQ chromatin analysis kit is a novel epigenetics research tool for the quantitative assessment of chromatin structure in cultured cells. By combining in situ chromatin digestion, genomic DNA purification, and real-time PCR, the chromatin state for several gene promoters can be studied simultaneously.

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