Protocols and Tips | Bio-Radiations

From Gel to Journal: A Practical Guide to Western Blot Submission

Bioradiations Staff — Thu, 05 Mar 2026 20:47:39 +0000

If you are getting ready to submit a research paper with western blot data, you’ve probably noticed that journals have specific—and often strict—guidelines for submitting digital images. To help you avoid last-minute headaches and uncertainty over whether ...

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Stain-Free Technology: Get Better Data in Less Time

Bioradiations Staff — Tue, 11 Nov 2025 17:56:32 +0000

Western blotting is a cornerstone of protein analysis—but the traditional workflow is slow, labor-intensive, and error-prone, plus it requires staining steps to visualize proteins on a gel or blot. Stain-Free ...

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10 Tips for Optimizing High-Throughput Flow Cytometry Assays

Bioradiations Staff — Tue, 11 Nov 2025 17:55:05 +0000

Flow cytometry can be labor intensive, and efforts to increase throughput can lead to reduced data quality or even instrument reliability issues such as blockages. While some instruments are more ...

The post 10 Tips for Optimizing High-Throughput Flow Cytometry Assays first appeared on Bio-Radiations.

10 Key Factors for Achieving Accurate Cell Counts: Tips, Tricks, and Myths

Bioradiations Staff — Tue, 12 Nov 2024 17:47:36 +0000

Counting is something we are all familiar with but it’s not always as simple as one, two, three. Measuring the concentration of cells in suspension is a common but surprisingly complex task in cell biology that is easy to get wrong. This article explores ten factors that can influence cell counting, gives advice on how to improve accuracy, and dispels some commonly held myths.

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Step into the Future of Blot and Gel Imaging with the ChemiDoc Go Imaging System

Bioradiations Staff — Tue, 13 Aug 2024 20:57:01 +0000

The ChemiDoc Go Imaging System, the newest in the ChemiDoc imaging line, effortlessly captures precise, high-sensitivity images of western blots and nucleic acid gels from a compact benchtop system — transforming lab workflows. Explore how this next-generation imager redefines your research capabilities across various applications, including multiplexing and stain-free protein detection.

The post Step into the Future of Blot and Gel Imaging with the ChemiDoc Go Imaging System first appeared on Bio-Radiations.

Maximize Genomic Insights by Combining Molecular Profiling Methods

Bioradiations Staff — Tue, 11 Jun 2024 19:35:52 +0000

Dive into an in-depth exploration of the molecular methods for gene expression profiling — highlighting the strengths, applications, and uses of quantitative PCR (qPCR), Droplet Digital PCR (ddPCR), and RNA sequencing (RNA-Seq) technologies.

The post Maximize Genomic Insights by Combining Molecular Profiling Methods first appeared on Bio-Radiations.

PCR Plates: 5 Tips to Guide Your Buying Decision

Bioradiations Staff — Tue, 08 Aug 2023 18:13:21 +0000

Learn about 5 key aspects of a PCR plate that are crucial to your results. These tips provide you with the tools to make an informed decision when purchasing PCR plates.

The post PCR Plates: 5 Tips to Guide Your Buying Decision first appeared on Bio-Radiations.

Quick Tips for Western Blotting

Bioradiations Staff — Tue, 14 Feb 2023 19:30:23 +0000

Learn how to improve your western blotting results with our Quick Tips video series. Watch these 1–2 minute videos see how to perfect each step of the western blotting process.

The post Quick Tips for Western Blotting first appeared on Bio-Radiations.

Resolved — Comic Series

Bioradiations Staff — Tue, 11 Jan 2022 07:56:37 +0000

Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.

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6 Tips to Secure Funding for Flow Cytometers

Bioradiations Staff — Wed, 09 Jun 2021 01:38:24 +0000

We asked core facilities staff and research scientists from the U.S. and Europe for their top tips for securing funding for large instrumentation, such as a cell analyzer or cell sorter. Here are the key questions you should consider while preparing your grant application to increase your chances of a successful outcome.

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Single-Step Column Purification for H1N1 VirusUsing Nuvia HP-Q AEX Resin

Bioradiations Staff — Wed, 09 Jun 2021 00:01:27 +0000

Learn how a team developed a single-step workflow for column purification of the Influenza A H1N1 subtype using the recently developed Nuvia HP-Q Anion Exchange Resin. This protocol is scalable, efficient, and produced good yields with high purity.

The post Single-Step Column Purification for H1N1 Virus
Using Nuvia HP-Q AEX Resin first appeared on Bio-Radiations.

Inflamed in the Brain: A Protocol for Studying Neuroinflammation in Mouse Brain Tissue

Bioradiations Staff — Tue, 08 Dec 2020 18:51:33 +0000

Mice are critical model organisms for the study of neuroinflammation. But tissue quantity is often a limiting factor. In a recent paper, researchers describe a protocol for analysis of 33 cytokines and chemokines using only 50 μl of tissue homogenate. The remaining homogenate can be used for RNA extraction or other analysis.

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7 Tips for Purchasing Your Next Cell Sorter

Bioradiations Staff — Wed, 09 Sep 2020 22:01:53 +0000

Presented here are some tips to help you avoid buyer’s remorse and make an educated decision about which instrument to purchase. These tips provide you with the tools to make an informed decision when purchasing a cell sorter.

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10 Tips for Setting Up a Successful Cell Sorting Experiment

Bioradiations Staff — Wed, 10 Jun 2020 17:59:14 +0000

Incorporating fluorescence-based cell sorting into your lab grants you powerful access to new research areas and rarer cell types without sacrificing efficiency or results. New, powerful techniques like CRISPR and single-cell analysis are enhanced by the isolation of specific cells. But isolating cell populations of the right quality and quantity is both challenging and time consuming. We offer some tips to set yourself up for success and get the most out of your sorts.

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Protein Thermal Shift Assays Made Easy with Bio-Rad’s Family of CFX Real-Time PCR Detection Systems

Bioradiations Staff — Wed, 13 Nov 2019 21:12:22 +0000

Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. See how Bio-Rad’s family of CFX Real-Time PCR Detection Systems can measure protein thermal stability with higher throughput and more buffer systems than traditional circular dichroism detection.

The post Protein Thermal Shift Assays Made Easy with
Bio-Rad’s Family of CFX Real-Time PCR Detection Systems first appeared on Bio-Radiations.

Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR

Bioradiations Staff — Wed, 11 Sep 2019 18:17:59 +0000

One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.

The post Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR first appeared on Bio-Radiations.

10 Tips for Western Blot Detection of Phosphorylation Events

Bioradiations Staff — Wed, 13 Feb 2019 18:21:10 +0000

Being able to interrogate protein phosphorylation events and aberrant phosphorylation levels is critical to our understanding of various biological and pathological processes. However, detection of phosphorylated proteins in western blots can be challenging. These ten tips will help you generate robust data and cleaner blots.

The post 10 Tips for Western Blot Detection of Phosphorylation Events first appeared on Bio-Radiations.

Six Tips to Improve Your Co-IP Results

Bioradiations Staff — Tue, 09 Oct 2018 23:18:56 +0000

Co-immunoprecipitation (co-IP) is a great way to identify target protein complexes and interacting partners. Though the technique can be highly informative, it is also challenging to master. The six tips and protocol outlined in this article will help you achieve successful co-IP results.

The post Six Tips to Improve Your Co-IP Results first appeared on Bio-Radiations.

10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates

Bioradiations Staff — Wed, 15 Aug 2018 18:33:06 +0000

Extracting proteins from tissue lysates for western blotting can be a challenge. From knowing how to process your tissue to verifying protein loading to including suitable controls, there is a lot to keep track of. The ten tips in this article can help you on your way to generating reproducible and reliable blots and do better western blotting overall.

The post 10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates first appeared on Bio-Radiations.

Got Purification Troubles?

Bioradiations Staff — Wed, 15 Aug 2018 14:49:39 +0000

Use our interactive infographic to find out how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification. And find out what you can do to minimize these on-column aggregates.

The post Got Purification Troubles? first appeared on Bio-Radiations.

Finding a Good Antibody: Part III – Publish Meaningful Data

Bioradiations Staff — Tue, 10 Jul 2018 21:30:26 +0000

In the first two articles in our Finding a Good Antibody series, you learned how to select the best antibodies and received tips on proper antibody validation. In this third and final article, see how you can use this information to publish meaningful data when using antibodies. And get some guidelines for antibody best practices.

The post Finding a Good Antibody:
Part III – Publish Meaningful Data first appeared on Bio-Radiations.

Finding a Good Antibody:Part II — Antibody Validation

Bioradiations Staff — Tue, 12 Jun 2018 22:41:34 +0000

Finding antibodies that work for your application is just the first step in finding a good antibody. Next is antibody validation — making sure that your antibody does what you think it is supposed to. In this second article in our Finding a Good Antibody series, learn how best to go about validating your antibodies.

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Part II — Antibody Validation first appeared on Bio-Radiations.

Finding a Good Antibody:Part I — Selecting the Best Antibodies

Bioradiations Staff — Wed, 09 May 2018 19:21:26 +0000

Even though antibodies are central to basic research as well as drug development and diagnostics, concerns with antibody validation remain high, and finding an antibody that works well for a specific application can be quite a challenge. Use these tips to select the best antibodies for your application.

The post Finding a Good Antibody:
Part I — Selecting the Best Antibodies first appeared on Bio-Radiations.

Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples

Bioradiations Staff — Wed, 14 Feb 2018 00:15:14 +0000

Incorporating preamplification into your qPCR experiments may seem daunting, but it is a powerful way to analyze targets from limited samples. The tips in this article will help you understand how preamplification can enhance your qPCR experiments and the considerations and implications for incorporating it into your workflow.

The post Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples first appeared on Bio-Radiations.

Tips to Make Fluorophore Picking Easier

Bioradiations Staff — Wed, 11 Oct 2017 23:40:49 +0000

Fluorescence is a property of photo-excitable molecules that has been exploited to develop dyes, or fluorophores, that are used in a variety of experimental procedures, including western blotting. Learn more about the science behind fluorescent dyes and get some helpful tips on how to pick the right fluorophores for your experimental needs.

The post Tips to Make Fluorophore Picking Easier first appeared on Bio-Radiations.

Key Steps to Ensure Optimized and Reproducible qPCR Experiments

Bioradiations Staff — Fri, 14 Apr 2017 00:37:07 +0000

Real-time PCR is a common and easy-to-adopt technology. By keeping a few simple steps in mind as you design and run your experiments you can ensure high-quality, reproducible data.

The post Key Steps to Ensure Optimized and Reproducible qPCR Experiments first appeared on Bio-Radiations.

Characterization of Critical Reagents for Ligand Binding Assays

Bioradiations Staff — Wed, 15 Feb 2017 22:29:13 +0000

Ligand binding assays are utilized throughout the large-molecule drug development process. However, their robustness, accuracy, and reproducibility depend on the quality of critical reagents. See how you can ensure their consistent characterization, particularly during early drug development stages.

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Multiplex Immunoassays: Five Tips for Optimizing Your Experiments

Bioradiations Staff — Fri, 13 Jan 2017 00:51:22 +0000

Follow the five tips in this article before embarking on your multiplex immunoassay journey to generate accurate, efficient, and biologically relevant results. From help designing experiments to acquiring accurate data, this article has it all.

The post Multiplex Immunoassays: Five Tips for Optimizing Your Experiments first appeared on Bio-Radiations.

Digital PCR: Helpful Tips When Using Droplet Partitioning Technology

Bioradiations Staff — Thu, 10 Nov 2016 00:43:28 +0000

Digital PCR offers a highly sensitive alternative to conventional qPCR, enabling absolute quantification of nucleic acids and rare allele detection. The tips in this article help you set up and optimize your digital PCR experiments when using droplet partitioning technology.

The post Digital PCR: Helpful Tips When Using Droplet Partitioning Technology first appeared on Bio-Radiations.

Ten Tips for Improving Western Blot Detection of Immunoprecipitation Samples

Bioradiations Staff — Thu, 10 Nov 2016 00:38:14 +0000

Immunoprecipitation (IP) experiments and the detection of IP samples by western blotting can be quite challenging at times. The tips in this article help you set up and optimize your overall IP experimenta design.

The post Ten Tips for Improving Western Blot Detection of Immunoprecipitation Samples first appeared on Bio-Radiations.

TGX Stain-Free™ FastCast™ Acrylamide Gel Casting

Bioradiations Staff — Thu, 10 Nov 2016 00:35:30 +0000

This short video demonstrates how you can access the unique benefits of Bio-Rad's proprietary TGX Stain-Free Gel chemistry by hand casting your own TGX Stain-Free Gels in just a few quick and easy steps.

The post TGX Stain-Free™ FastCast™ Acrylamide Gel Casting first appeared on Bio-Radiations.

Cleaning the Nozzle Tip on the S3e™ Cell Sorter

Bioradiations Staff — Thu, 15 Sep 2016 23:20:54 +0000

In this short video, we show how to clean the nozzle tip on the S3e Cell Sorter.

The post Cleaning the Nozzle Tip on the S3e™ Cell Sorter first appeared on Bio-Radiations.

Six Tips for Efficient Transfer of Your Proteins

Bioradiations Staff — Thu, 15 Sep 2016 23:14:49 +0000

A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.

The post Six Tips for Efficient Transfer of Your Proteins first appeared on Bio-Radiations.

Performing a Fluidics Hot Swap on the S3e™ Cell Sorter

Bioradiations Staff — Thu, 15 Sep 2016 23:13:47 +0000

In this video, we show how a fluidics hot swap can be performed without shutting down the S3e Cell Sorter.

The post Performing a Fluidics Hot Swap on the S3e™ Cell Sorter first appeared on Bio-Radiations.

Cleaning the Streams Camera Window on the S3e™ Cell Sorter

Bioradiations Staff — Thu, 15 Sep 2016 23:10:43 +0000

In this video we show you how to clean the camera streams window as part of routine maintenance for your S3e Cell Sorter.

The post Cleaning the Streams Camera Window on the S3e™ Cell Sorter first appeared on Bio-Radiations.

Attaching a Dynaloop to the NGC™ Chromatography System

Bioradiations Staff — Fri, 12 Aug 2016 04:47:07 +0000

This video describes the three main steps in attaching a DynaLoop Sample Loop to the NGC™ Chromatography System to enable the injection of larger sample volumes: connecting the DynaLoop Sample Loop to the NGC Chromatography System, priming the DynaLoop Sample Loop, and creating a method in ChromLab™ Software.

The post Attaching a Dynaloop to the NGC™ Chromatography System first appeared on Bio-Radiations.

Calibrating the pH Valve on the NGC™ Chromatography System

Bioradiations Staff — Fri, 12 Aug 2016 04:44:47 +0000

The NGC pH valve provides real-time, in-line monitoring of buffer pH. In this video, you’ll learn how to connect the pH valve to the NGC System, add it to the flow path, and calibrate the valve using buffers at two different pH values. Features of the pH valve include accessible calibration ports and easy bypass of the probe when the NGC System is being cleaned.

The post Calibrating the pH Valve on the NGC™ Chromatography System first appeared on Bio-Radiations.

Tips and Strategies for Successful Purification of Your Proteins

Bioradiations Staff — Fri, 12 Aug 2016 04:36:16 +0000

A biomolecule protein purification workflow requires more than one resin. The trick is to find the combination of resins that provides the highest purity in the least number of steps and at the lowest cost. Here are some guides to select the optimal resins for the purification of monoclonal antibodies, native and recombinant proteins, Ig G and mammalian and adenovirus, and for removing aggregates and endotoxins.

The post Tips and Strategies for Successful Purification of Your Proteins first appeared on Bio-Radiations.

Removing Air Bubbles from the NGC™ Chromatography System

Bioradiations Staff — Fri, 15 Jul 2016 21:21:39 +0000

This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles.

The post Removing Air Bubbles from the NGC™ Chromatography System first appeared on Bio-Radiations.

Using the Air Sensor on the NGC™ Chromatography System

Bioradiations Staff — Fri, 15 Jul 2016 21:09:24 +0000

This video describes the functions of the Air Sensor Module and how to connect it to the NGC Chromatography System. The NGC Air Sensor senses the end of sample or buffer and prevents the introduction of air into the system, which can damage columns and/or incur the loss of precious samples. Both small and large air sensors are available to fit the tubing being used. Steps for connecting the Air Sensor Module and associated air sensors are described including how to add the air sensors into the system flow path using ChromLab™ Software and the differences in using the air sensors in manual vs. method modes.

The post Using the Air Sensor on the NGC™ Chromatography System first appeared on Bio-Radiations.

A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates

Bioradiations Staff — Thu, 16 Jun 2016 23:33:14 +0000

It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.

The post A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates first appeared on Bio-Radiations.

Tips for Selecting the Best Gel for Your Application

Bioradiations Staff — Thu, 16 Jun 2016 23:05:15 +0000

Picking the right gel for your application is not easy. It depends on so many factors, such as the purpose of your application, the size of your protein, what kind of buffer you need to have in your sample etc. Here we provide a guide for you to choose the best Bio-Rad gel based on all these factors.

The post Tips for Selecting the Best Gel for Your Application first appeared on Bio-Radiations.

8 Tips for Understanding Multiplex Immunoassay Development and Validation

Bioradiations Staff — Tue, 08 Mar 2016 18:40:39 +0000

Multiplex immunoassay development requires a lot of validation and standardization steps. Several factors can influence the performance of these assays. Some factors that need to be considered are mentioned here.

The post 8 Tips for Understanding Multiplex Immunoassay Development and Validation first appeared on Bio-Radiations.

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Bioradiations Staff — Tue, 09 Feb 2016 21:47:40 +0000

Watch this video to see how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

The post Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies first appeared on Bio-Radiations.

List of Mouse Lineage Biomarkers for Different Immune Cell Types

Bioradiations Staff — Wed, 13 Jan 2016 01:54:06 +0000

How can we easily identify biomarkers for different immune cell types? This color-coded poster makes identification of mouse lineage biomarkers easy.

The post List of Mouse Lineage Biomarkers for Different Immune Cell Types first appeared on Bio-Radiations.

Chromatography: Everything You Need to Know about Your Peaks

Bioradiations Staff — Wed, 13 Jan 2016 01:53:29 +0000

What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.

The post Chromatography: Everything You Need to Know about Your Peaks first appeared on Bio-Radiations.

Flow Cytometry: Basic Definitions

Bioradiations Staff — Tue, 08 Dec 2015 19:07:09 +0000

It’s always good to get the basics straight. Here, we’ve defined some flow cytometry terms that might have eluded you.

The post Flow Cytometry: Basic Definitions first appeared on Bio-Radiations.

High-Processivity Sso7d Fusion Polymerase Improves qPCR Results

Bioradiations Staff — Tue, 08 Dec 2015 19:01:56 +0000

Processivity is the rate at which nucleotides are added to extend PCR products. High processivity results in a greater number of nucleotides incorporated in each polymerase binding event.

The post High-Processivity Sso7d Fusion Polymerase Improves qPCR Results first appeared on Bio-Radiations.

10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels

Bioradiations Staff — Tue, 10 Nov 2015 22:30:16 +0000

Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.

The post 10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels first appeared on Bio-Radiations.

Protocol: Preparation of Cells for Flow Cytometry

Bioradiations Staff — Wed, 14 Oct 2015 00:34:44 +0000

Harvesting of tissue culture cells could be challenging. Here is an easy protocol for efficient harvesting of cells for flow cytometry analysis.

The post Protocol: Preparation of Cells for Flow Cytometry first appeared on Bio-Radiations.

Four Tips for Running the Perfect SDS-PAGE Gel

Bioradiations Staff — Tue, 11 Aug 2015 22:25:07 +0000

We share five simple tricks that address common gel running woes, from samples that float out of wells to sample buffers that turn yellow.

The post Four Tips for Running the Perfect SDS-PAGE Gel first appeared on Bio-Radiations.

Get Published! 10 Tips for Getting Published Successfully

Bioradiations Staff — Tue, 11 Aug 2015 09:34:26 +0000

When you have finished all your experiments and have the paper ready in your head, you need to consider several factors before sending it for publication. Here are some tips that Bio-Rad’s PrimePCR™ PCR Primers and Assays group put together for getting the paper published successfully.

The post Get Published! 10 Tips for Getting Published Successfully first appeared on Bio-Radiations.

Automated Multi-D Chromatography on the NGC™ System

Bioradiations Staff — Wed, 15 Jul 2015 00:01:04 +0000

What is Multidimensional (Multi-D) chromatography? Here is an animated explanation of how Multi-D chromatography works and what its advantages are over traditional chromatography.

The post Automated Multi-D Chromatography on the NGC™ System first appeared on Bio-Radiations.

7 Tips for Choosing the Right Antibody for Western Blotting

Bioradiations Staff — Tue, 09 Jun 2015 22:40:56 +0000

Which antibody is the best to obtain a definitive and conclusive answer for my questions? How do I choose the best antibody for my purpose? These are standard questions that scientists ask time and time again. Here are a few tips to choose the most appropriate antibody for your specific application.

The post 7 Tips for Choosing the Right Antibody for Western Blotting first appeared on Bio-Radiations.

Cell Imaging Simplified – the ZOE™ Fluorescent Cell Imager

Bioradiations Staff — Wed, 20 May 2015 22:41:58 +0000

The ZOE fluorescent cell imager features an integrated digital camera and touch-screen interface that makes brightfield and multichannel fluorescence microscopy simple, fast, and intuitive. An easy-to-use alternative to the traditional inverted microscope, ZOE eliminates the complexities of multichannel fluorescence imaging by combining the ease of use of a personal tablet and the power of an inverted microscope.

The post Cell Imaging Simplified – the ZOE™ Fluorescent Cell Imager first appeared on Bio-Radiations.

SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol

Bioradiations Staff — Mon, 11 May 2015 21:00:43 +0000

Immunoprecipitation (IP) is a commonly used protocol for concentrating and purifying proteins using specific antibodies and magnetic beads. Here is a simple IP protocol using Bio-Rad’s SureBeads Magnetic Beads.

The post SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol first appeared on Bio-Radiations.

PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells – A Fast Approach to Staining Nuclei

Bioradiations Staff — Mon, 12 Jan 2015 20:00:47 +0000

PureBlu Hoechst 33342 Nuclear Staining Dye is the optimal choice for staining the nuclei of live cells for ultraviolet imaging. This short video goes through the steps involved in staining live cells with PureBlu Hoechst 33342 Nuclear Staining Dye.

The post PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells – A Fast Approach to Staining Nuclei first appeared on Bio-Radiations.

PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate

Bioradiations Staff — Mon, 12 Jan 2015 20:00:45 +0000

One easy way to obtain a PrimePCR plate customized for your research is to modify one of Bio-Rad’s predesigned panels. This short video tutorial shows you how to use the online tools at Bio-Rad.com to edit a predesigned plate and create, then order your own customized 96- or 384-well PCR plate.

The post PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate first appeared on Bio-Radiations.

How to Build a Custom PrimePCR™ Real-Time PCR Plate Using a Suggested or Blank Template

Bioradiations Staff — Mon, 12 Jan 2015 20:00:45 +0000

It’s fast and easy to build your own PrimePCR plate. This video walks you through using the Custom Plate Configurator at Bio-Rad.com to create your own 96- or 384-well PCR plate.

The post How to Build a Custom PrimePCR™ Real-Time PCR Plate Using a Suggested or Blank Template first appeared on Bio-Radiations.

PureBlu™ DAPI Nuclear Staining Dye for Fixed Cells – A Fast Approach to Staining Nuclei

Bioradiations Staff — Mon, 12 Jan 2015 20:00:43 +0000

PureBlu DAPI Nuclear Staining Dye is the optimal choice for staining the nuclei of fixed cells for ultraviolet imaging. This short video goes through the steps involved in staining live cells with PureBlu DAPI Nuclear Staining Dye.

The post PureBlu™ DAPI Nuclear Staining Dye for Fixed Cells – A Fast Approach to Staining Nuclei first appeared on Bio-Radiations.

How to Order PrimePCR™ Assays – Primers, Probes, and Panels

Bioradiations Staff — Tue, 09 Dec 2014 20:00:48 +0000

A wide range of PCR assays is available through Bio-Rad.com for use with real-time or Droplet Digital™ PCR systems. Get a quick refresher on ordering these products with this short video.

The post How to Order PrimePCR™ Assays – Primers, Probes, and Panels first appeared on Bio-Radiations.

Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: Device Time-Out

Bioradiations Staff — Thu, 12 Jun 2014 19:00:40 +0000

A “device time-out” message on the Bio-Plex^® 200 System occurs when the instrument’s needle or probe cannot move correctly. Watch this video for several troubleshooting steps to try when this message occurs.

The post Troubleshooting the Bio-Plex^® 200 Multiplex Immunoassay System: Device Time-Out first appeared on Bio-Radiations.

Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: HTF Alarm

Bioradiations Staff — Tue, 10 Jun 2014 19:00:30 +0000

The high-throughput fluidics (HTF) alarm on the Bio-Plex^® 200 System indicates a problem with the instrument’s sheath fluid delivery system. See the actions needed to quickly resolve potential issues with this system and get back to your research!

The post Troubleshooting the Bio-Plex^® 200 Multiplex Immunoassay System: HTF Alarm first appeared on Bio-Radiations.

Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: Device Failed to Pressurize

Bioradiations Staff — Tue, 10 Jun 2014 17:55:20 +0000

During calibration of your Bio-Plex^® 200 System, you may receive a “failed to pressurize message.” In this short video, get some easy fixes for the most common issues prompting this message.

The post Troubleshooting the Bio-Plex^® 200 Multiplex Immunoassay System: Device Failed to Pressurize first appeared on Bio-Radiations.

How to Remove Stripes from a Gel Image — ChemiDoc™ Imager Filter Alignment

Bioradiations Staff — Sat, 31 May 2014 23:10:56 +0000

Usually when stripes appear on a gel image produced with a ChemiDoc XRS+ or ChemiDoc MP Imaging System, a misaligned filter is the cause. Learn the simple steps to correct this issue with this video from Bench Partners.

The post How to Remove Stripes from a Gel Image — ChemiDoc™ Imager Filter Alignment first appeared on Bio-Radiations.

Ligand Immobilization in Protein Interaction Studies — An Unattended Amine Coupling Protocol with Automatic Coinjection Activation

Bioradiations Staff — Fri, 16 May 2014 19:00:40 +0000

Amine coupling is the most commonly adopted technique to bind proteins to biosensor chips. Because of the possibility of the amine coupling reagents reacting with each other, these have to be premixed just before the injection and injected independently. Here are some tips to automatically coinject these reagents.

The post Ligand Immobilization in Protein Interaction Studies — An Unattended Amine Coupling Protocol with Automatic Coinjection Activation first appeared on Bio-Radiations.

DIY Cell Sorting with Bio-Rad’s S3™ Cell Sorter

Bioradiations Staff — Tue, 08 Apr 2014 17:13:56 +0000

Until recently, cell sorting has been a complex endeavor requiring specialized facilities and trained technicians. This infographic reveals how Bio-Rad’s S3 cell sorter makes this technique available to individual, non-expert researchers in 3 easy steps.

The post DIY Cell Sorting with Bio-Rad’s S3™ Cell Sorter first appeared on Bio-Radiations.

TC20™ and TC10™ Cell Counters – Cleaning the Print Heads for Clear Printed Results

Bioradiations Staff — Mon, 07 Apr 2014 20:11:39 +0000

Most problems with misaligned printouts on the printer supplied with the TC20 and TC10 cell counters can be solved by cleaning the print heads. In this short video, find out the fast, easy, and effective way to clean these print heads so you can get back to your research!

The post TC20™ and TC10™ Cell Counters – Cleaning the Print Heads for Clear Printed Results first appeared on Bio-Radiations.

Bio-Rad Supply Center — How-To Tips for Setting Up

Bioradiations Staff — Tue, 11 Mar 2014 22:09:50 +0000

Learn the best practices and tips for setting up your Bio-Rad Supply Center.

The post Bio-Rad Supply Center — How-To Tips for Setting Up first appeared on Bio-Radiations.

Bio-Rad Supply Center — How-To Tips for Taking Inventory

Bioradiations Staff — Tue, 11 Mar 2014 20:46:07 +0000

In this new video series, Bio-Rad offers answers for some your most frequently asked questions. This installment covers setting up and taking inventory of your Bio-Rad Supply Center.

The post Bio-Rad Supply Center — How-To Tips for Taking Inventory first appeared on Bio-Radiations.

Casting Polyacrylamide Gels with TGX™ and TGX Stain-Free™ FastCast™ Acrylamide Kits

Bioradiations Staff — Tue, 14 Jan 2014 20:19:42 +0000

Want a streamlined, easy workflow for hand casting polyacrylamide gels? This tutorial video shows how to use Bio-Rad’s new FastCast™ acrylamide kits to hand cast gels, saving time while providing the benefits of the latest precast gel chemistries.

The post Casting Polyacrylamide Gels with TGX™ and TGX Stain-Free™ FastCast™ Acrylamide Kits first appeared on Bio-Radiations.

Guide to SPR Data Processing on the ProteOn™ XPR36 System

Bioradiations Staff — Tue, 13 Aug 2013 19:00:00 +0000

Good data processing practice ensures that protein interaction experiments using a surface plasmon resonance (SPR) system will yield the clearest possible results. Here we present tips for understanding and processing SPR sensorgrams when using Bio-Rad’s ProteOn™ XPR36 protein interaction array system.

The post Guide to SPR Data Processing on the ProteOn™ XPR36 System first appeared on Bio-Radiations.

Guide to SPR Data Analysis on the ProteOn™ XPR36 System

Bioradiations Staff — Tue, 09 Jul 2013 19:00:28 +0000

Analyzing protein interaction data using a surface plasmon resonance (SPR) system can be complicated. This user guide describes how to analyze SPR data to obtain kinetic and equilibrium constants, as well as sample concentrations.

The post Guide to SPR Data Analysis on the ProteOn™ XPR36 System first appeared on Bio-Radiations.

Options for Dataset Export Using the ProteOn Manager™ Software

Bioradiations Staff — Tue, 09 Jul 2013 16:34:56 +0000

This appendix to “Guide to SPR Data Analysis on the Proteon™ XPR36 System” gives information on the data set export using ProteOn Manager™ Software.

The post Options for Dataset Export Using the ProteOn Manager™ Software first appeared on Bio-Radiations.

Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting

Bioradiations Staff — Tue, 23 Apr 2013 01:00:00 +0000

2-D electrophoresis followed by immunodetection of protein spots using western blotting is adopted commonly in proteomics studies. Here we present step-by-step instructions for obtaining accurate overlaps between the gel and the blot using the PDQuest 2-D gel analysis software.

The post Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting first appeared on Bio-Radiations.

A Novel Biotinylated Ligand-Capture Method with Surface Regeneration Capability for Label-Free Biomolecular Interaction Analysis

Bioradiations Staff — Tue, 12 Feb 2013 20:00:47 +0000

Ligand-capture approaches using biotin as an affinity tag are often utilized in biomolecular interaction analyses with surface plasmon resonance (SPR) biosensors. However, a drawback to using biotin is that it makes surface regeneration of SPR sensor chips difficult. Here is a strategy that facilitates surface regeneration when using biotinylated ligand-capture approaches to SPR analysis.

The post A Novel Biotinylated Ligand-Capture Method with Surface Regeneration Capability for Label-Free Biomolecular Interaction Analysis first appeared on Bio-Radiations.

Analyzing Binding Kinetics with Surface Plasmon Resonance Complemented with Direct Mass Spectrometry on the Same Sensor Chip

Bioradiations Staff — Mon, 07 Jan 2013 20:00:23 +0000

Surface Plasmon Resonance (SPR) is an optical phenomenon that is used for the label-free analysis of the binding of any two molecules in real-time.

The post Analyzing Binding Kinetics with Surface Plasmon Resonance Complemented with Direct Mass Spectrometry on the Same Sensor Chip first appeared on Bio-Radiations.

PDQuest™ Software: Bite-Sized Video Tutorials Make Learning 2-D Analysis Easy

Bioradiations Staff — Wed, 18 Jul 2012 19:10:36 +0000

Learning to use analysis software can be time-consuming and following a manual can be challenging.

The post PDQuest™ Software: Bite-Sized Video Tutorials Make Learning 2-D Analysis Easy first appeared on Bio-Radiations.

PDQuest™ Software Tutorials

Bioradiations Staff — Wed, 18 Jul 2012 19:00:24 +0000

The nine PDQuest tutorials are chapterized so you can focus on what you want to learn. Each video runs from 3 to 9 minutes and features callout boxes that highlight important information to complement the audio explanations.

The post PDQuest™ Software Tutorials first appeared on Bio-Radiations.

Video Tutorial for Analyzing Binding Interactions with Histidine-Tagged Proteins

Bioradiations Staff — Tue, 22 May 2012 19:00:35 +0000

Analyzing antibody binding to histidine-tagged proteins is not always easy because of inherent problems such as nonspecific binding and loss of analyte response associated with these tagged proteins. Optimization of these factors is key to precisely measuring the binding kinetics of these proteins.

The post Video Tutorial for Analyzing Binding Interactions with Histidine-Tagged Proteins first appeared on Bio-Radiations.

Bio-Rad Automated Supply Center in Action

Bioradiations Staff — Fri, 10 Feb 2012 20:00:09 +0000

Automated supply centers reduce paper and packaging waste, apart from providing convenience. As part of its sustainability efforts, Bio-Rad is increasing the number of its supply center sites. The video describes how the supply center works.

The post Bio-Rad Automated Supply Center in Action first appeared on Bio-Radiations.

Transitioning from Chemiluminescent to Multiplex Fluorescent Blotting: Things to Consider

Bioradiations Staff — Thu, 26 Jan 2012 22:12:02 +0000

Today many researchers are considering changing their western blot detection method from chemiluminescence to multiplex fluorescence. There are several drivers behind this trend. Most significantly, fluorescent detection allows users to multiplex their western blots, enabling simultaneous detection of several target proteins at once, reducing or eliminating the need to strip and re-probe. Other benefits of fluorescence include better dynamic range, more quantitative results, and better signal stability over time.

The post Transitioning from Chemiluminescent to Multiplex Fluorescent Blotting: Things to Consider first appeared on Bio-Radiations.

PROTEAN® i12™ IEF System Introduction

Bioradiations Staff — Mon, 12 Dec 2011 20:00:30 +0000

Bio-Rad’s PROTEAN i12 IEF system is specially designed for running different sample types, different gradients, and multiple 2-D electrophoresis protocols at the same time. Watch the video to learn about all the new features of this IEF system.

The post PROTEAN^® i12™ IEF System Introduction first appeared on Bio-Radiations.

Innovating Electrophoresis with Precast Gels

Bioradiations Staff — Fri, 26 Aug 2011 19:00:59 +0000

Setting new standards for consistency and speed in the electrophoresis workflow.

The post Innovating Electrophoresis with Precast Gels first appeared on Bio-Radiations.

Trans-Blot Turbo Instructional Video

Bioradiations Staff — Wed, 24 Aug 2011 19:00:02 +0000

Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.

The post Trans-Blot Turbo Instructional Video first appeared on Bio-Radiations.

Trans-Blot® Turbo™ System vs. the iBlot System

Bioradiations Staff — Tue, 23 Aug 2011 19:00:44 +0000

Battle of the blotters: see how the Trans-Blot Turbo system performs compared to the iBlot dry blotting system.

The post Trans-Blot^® Turbo™ System vs. the iBlot System first appeared on Bio-Radiations.

Trans-Blot® Turbo™ System vs. Traditional Blotting Techniques

Bioradiations Staff — Mon, 22 Aug 2011 19:00:37 +0000

See how the Trans-Blot Turbo system measures up against traditional blotting techniques.

The post Trans-Blot^® Turbo™ System vs. Traditional Blotting Techniques first appeared on Bio-Radiations.

Bio-Plex® Assay Configurations Simplified with the Bio-Plex Assay Builder for Complete Flexibility

Bioradiations Staff — Mon, 13 Jun 2011 19:00:55 +0000

Now you can optimize research expenditure by multiplexing only the targets you need to get the answers you're seeking. This newly updated tool allows you to choose from within panels to build the perfect kit.

The post Bio-Plex^® Assay Configurations Simplified with the Bio-Plex Assay Builder for Complete Flexibility first appeared on Bio-Radiations.

Guidelines for Developing Robust and Reproducible High-Resolution Melt Analysis Assays

Bioradiations Staff — Mon, 13 Jun 2011 19:00:40 +0000

Classifying and understanding genetic variation between populations and individuals is an important aim in the field of genomics. High resolution melt (HRM) analysis is the quantitative analysis of the melt curve of a DNA fragment following amplification by PCR and can be considered the next-generation application of amplicon melting analysis. Careful sample preparation and planning of experimental and assay design are crucial for robust and reproducible results.

The post Guidelines for Developing Robust and Reproducible High-Resolution Melt Analysis Assays first appeared on Bio-Radiations.

Using the Precision Plus Protein™ WesternC™ Standard as a QC Tool at Key Steps in Your Electrophoresis and Blotting Workflow

Bioradiations Staff — Mon, 13 Jun 2011 16:16:18 +0000

As a fundamental tool in protein research, it is critical that all steps of the electrophoresis workflow (from running gels to visualizing blots) are performed successfully to ensure accurate, reliable, and reproducible results. Protein standards are often used to monitor electrophoretic separation and transfer efficiency but do not always enable ladder visualization after blot development.

The post Using the Precision Plus Protein™ WesternC™ Standard as a QC Tool at Key Steps in Your Electrophoresis and Blotting Workflow first appeared on Bio-Radiations.