Droplet Digital PCR (ddPCR) | Bio-Radiations https://www.bioradiations.com A Resource for Life Science Research Mon, 27 Apr 2026 20:16:19 +0000 en-US hourly 1 https://www.bioradiations.com/wp-content/uploads/2025/10/BR-knocked-out-60x60.png Droplet Digital PCR (ddPCR) | Bio-Radiations https://www.bioradiations.com 32 32 Each month, join Kashef Qaadri, a biologist turned bioinformatician, as he interviews guests exploring real-world research informatics challenges through use-cases, providing insights and strategies for integrating and analyzing complex data that are driving BioPharma R&D. Bio-Rad Laboratories, Inc. false Bio-Rad Laboratories, Inc. lsgmarcomapps@gmail.com Copyright © 2019 Bio-Rad Laboratories, Inc. All rights reserved. Copyright © 2019 Bio-Rad Laboratories, Inc. All rights reserved. podcast Each month, join Kashef Qaadri, a biologist turned bioinformatician, as he interviews guests exploring real-world research informatics challenges through use-cases, providing insights and strategies for integrating and analyzing complex data that are d... Droplet Digital PCR (ddPCR) | Bio-Radiations https://www.bioradiations.com/wp-content/uploads/powerpress/bioradio_podsq_1400x1400-400.jpg https://www.bioradiations.com/digital-droplet-pcr-ddpcr/ Gene Editing: Navigating the Path from Innovation to Application https://www.bioradiations.com/gene-editing-navigating-the-path-from-innovation-to-application/ Mon, 27 Apr 2026 20:16:19 +0000 https://www.bioradiations.com/?p=13050 Gene editing is rapidly evolving, and its relevance across biotechnology and medicine continues to expand. As these tools advance, so does the need for reliable ways to measure editing efficiency ...

The post Gene Editing: Navigating the Path from Innovation to Application first appeared on Bio-Radiations.]]> qPCR vs. ddPCR™ Assays: Select the Best Gene Expression Analysis Method for Your Application https://www.bioradiations.com/qpcr-vs-ddpcr-assays-select-the-best-gene-expression-analysis-method-for-your-application1125/ Tue, 11 Nov 2025 17:57:13 +0000 https://www.bioradiations.com/?p=12923 Unsure whether to choose quantitative PCR (qPCR) or Droplet Digital™ PCR (ddPCR) for gene expression analysis? Bio-Rad’s new objective study comparing the two methods shows that the best choice depends ...

The post qPCR vs. ddPCR™ Assays: Select the Best Gene Expression Analysis Method for Your Application first appeared on Bio-Radiations.]]> Vive la Différence: The Beauty of Diversity in Life, Life Science, and Flow Cytometry https://www.bioradiations.com/the-beauty-of-diversity-in-flow-cytometry-225/ Tue, 11 Feb 2025 19:23:13 +0000 https://www.bioradiations.com/?p=12712 The differences between us are what makes life interesting, and diversity in a group that shares a common goal can lead to many benefits. Here we examine the similarities and differences between researchers using flow cytometry for academic research and those using it for drug discovery. We outline the challenges they face and learn that they share a common goal as well as a common solution.

The post Vive la Différence: The Beauty of Diversity in Life, Life Science, and Flow Cytometry first appeared on Bio-Radiations.]]> Global Lung Cancer Expert Dr. Ed Schuuring on How ddPCR Technology Powers ctDNA Analysis https://www.bioradiations.com/how-ddpcr-technology-powers-ctdna-analysis-225/ Tue, 11 Feb 2025 18:43:53 +0000 https://www.bioradiations.com/?p=12703 Characterizing biomarkers in lung cancer tumors is challenging due to the high risks associated with tissue retrieval. Liquid biopsy offers a safer alternative but requires highly sensitive and precise methods to generate robust results from minimal genetic material. In this video, Dr. Schuuring of the University Medical Center Groningen discusses how Droplet Digital PCR (ddPCR) is used in his laboratories and why this technology has become critical for his research.

The post Global Lung Cancer Expert Dr. Ed Schuuring on How ddPCR Technology Powers ctDNA Analysis first appeared on Bio-Radiations.]]> Insights from Droplet Digital PCR World 2024 https://www.bioradiations.com/insights-from-droplet-digital-pcr-world-2024-1124/ Tue, 12 Nov 2024 17:40:10 +0000 https://www.bioradiations.com/?p=12655 In June, Bio-Rad hosted Droplet Digital PCR (ddPCR) World 2024, the premier digital PCR event bringing together global leaders in science and innovation. ddPCR World features experts showcasing how ddPCR technology has been instrumental in overcoming some of their most complex research and manufacturing challenges. In this article, we are excited to share key insights and cutting-edge research using ddPCR technology that are driving scientific breakthroughs across the globe.

The post Insights from Droplet Digital PCR World 2024 first appeared on Bio-Radiations.]]> More to Learn from ESR1 Using ctDNA Analysis https://www.bioradiations.com/more-to-learn-from-esr1-using-ctdna-analysis-824/ Tue, 13 Aug 2024 21:18:36 +0000 https://www.bioradiations.com/?p=12610 Estrogen receptor 1 (ESR1) mutations in breast tumors were first identified nearly 30 years ago. Since then, technology has evolved, as has our understanding of the impact these mutations have on tumor cell physiology. Given the dynamic nature of ESR1 mutation acquisition and enrichment, ctDNA samples appear to be the most robust source for analysis. Droplet Digital PCR (ddPCR) enables rapid, highly sensitive, cost-effective analysis of ctDNA, providing a powerful tool for this critical area of cancer research.

The post More to Learn from ESR1 Using ctDNA Analysis first appeared on Bio-Radiations.]]> Unlocking the Potential of Cell and Gene Therapies: Overcoming Production Challenges https://www.bioradiations.com/cell-and-gene-therapy-production-challenges-824/ Tue, 13 Aug 2024 19:37:47 +0000 https://www.bioradiations.com/?p=12594 A common challenge in scaling up cell and gene therapy production is monitoring for contaminants, such as Mycoplasma. Learn how ddPCR can help in this webinar.

The post Unlocking the Potential of Cell and Gene Therapies: Overcoming Production Challenges first appeared on Bio-Radiations.]]> Guidance for the Validation of qPCR and ddPCR Assays in Regulated Environments https://www.bioradiations.com/qpcr-and-ddpcr-assay-validation-in-regulated-environments-624/ Tue, 11 Jun 2024 17:46:49 +0000 https://www.bioradiations.com/?p=12542 In this webinar, learn about validating qPCR and ddPCR assays in regulated environments. Explore validation parameters and regulatory considerations.

The post Guidance for the Validation of qPCR and ddPCR Assays in Regulated Environments first appeared on Bio-Radiations.]]> Replication-Competent Virus Testing: A Critical Step in Cell and Gene Therapy Quality Control https://www.bioradiations.com/replication-competent-virus-testing-for-qc-224/ Mon, 12 Feb 2024 18:19:18 +0000 https://www.bioradiations.com/?p=12522 Scientists commonly use lentiviruses and adeno-associated viruses (AAVs) as viral vectors in cell and gene therapy (CGT) production. However, therapies that use these viruses must undergo strict quality control measures, including testing for replication-competent viruses (RCV), to ensure that the treatment is safe. Cell culture-based methods are currently the gold standard for RCV testing, but it can take up to 45 days for results to be returned. Droplet Digital PCR (ddPCR) kits now offer an attractive orthogonal method.

The post Replication-Competent Virus Testing: A Critical Step in Cell and Gene Therapy Quality Control first appeared on Bio-Radiations.]]> Enhancing the Power of Microsatellite Instability Detection with ddPCR Technology https://www.bioradiations.com/enhance-the-power-of-msi-detection-with-ddpcr-1123/ Tue, 14 Nov 2023 20:07:21 +0000 https://www.bioradiations.com/?p=12478 As the third most prevalent cancer worldwide, colorectal cancer (CRC) is a significant global health burden. Biomarker identification and detection has proven a powerful strategy for improving CRC diagnosis and informing targeted treatment. Microsatellite instability (MSI) is a key biomarker in CRC, and with Droplet Digital PCR (ddPCR), MSI can be accurately detected using a wider range of sample types.

The post Enhancing the Power of Microsatellite Instability Detection with ddPCR Technology first appeared on Bio-Radiations.]]> Did You Know That Beyond Sensitivity, ddPCR Provides Unrivaled Precision and Accuracy? https://www.bioradiations.com/ddpcr-provides-unrivaled-precision-and-accuracy-1123/ Tue, 14 Nov 2023 19:53:26 +0000 https://www.bioradiations.com/?p=12474 Methods that are highly accurate and precise provide the right answer, consistently. For digital PCR (dPCR), Poisson statistics dictate that the accuracy and precision of results are dependent on the accuracy and precision of sample partitions. This means that to get reliable dPCR results, you need to know your partition volume accurately and your partition volumes need to be consistent. See how our Droplet Digital PCR technology provides both accuracy and precision.

The post Did You Know That Beyond Sensitivity, ddPCR Provides Unrivaled Precision and Accuracy? first appeared on Bio-Radiations.]]> Explore the Epigenomic Landscape of Single Cells with the scATAC You Seq https://www.bioradiations.com/explore-the-epigenomic-landscape-of-single-cells-1123/ Tue, 14 Nov 2023 19:39:16 +0000 https://www.bioradiations.com/?p=12470 Gain access to high-performance chromatin profiling to unravel the complexities of cellular heterogeneity, differentiation, and disease-specific alterations in gene regulation. With single-cell ATAC-seq (assay for transposase-accessible chromatin using sequencing), you can delve into the gene regulatory landscape of individual cells, unlocking invaluable insights into the epigenomic mechanisms that shape their behavior.

The post Explore the Epigenomic Landscape of Single Cells with the scATAC You Seq first appeared on Bio-Radiations.]]> Poly(A) Tails: A Critical Quality Attribute in mRNA-based Therapeutics https://www.bioradiations.com/polya-tails-in-mrna-based-therapeutics-823/ Tue, 08 Aug 2023 18:30:23 +0000 https://www.bioradiations.com/?p=12419 The therapeutic potential of messenger RNA (mRNA) therapeutics has an unprecedented ability to target previously undruggable diseases. The poly(A) tail drives the therapeutic efficacy of the mRNA molecule. Using Droplet Digital PCR (ddPCR) technology to analyze poly(A) tail length and integrity yields unrivaled sensitivity, enabling developers to ensure high poly(A) tail integrity and, ultimately, functional therapeutic mRNAs.

The post Poly(A) Tails: A Critical Quality Attribute in mRNA-based Therapeutics first appeared on Bio-Radiations.]]> Accurate Titering of Lentiviral Vectors is Paramount for Biotherapeutic Success https://www.bioradiations.com/accurate-titering-of-lentiviral-vectors-823/ Mon, 07 Aug 2023 20:48:37 +0000 https://www.bioradiations.com/?p=12399 Lentiviral vectors (LVVs) have become the primary method of gene delivery for cells ex vivo in both academic and clinical applications. Currently, qPCR is the gold standard for functional titering of LVVs, but Droplet Digital PCR (ddPCR) offers an attractive alternative due to its inherent benefits, such as absolute quantification without the use of a standard curve and enhanced resistance to PCR inhibitors.

The post Accurate Titering of Lentiviral Vectors is Paramount for Biotherapeutic Success first appeared on Bio-Radiations.]]> Achieving True Excellence in RNA Therapeutic Development https://www.bioradiations.com/achieving-excellence-in-rna-therapeutics-523/ Wed, 10 May 2023 08:09:48 +0000 https://www.bioradiations.com/?p=12346 The realm of druggable targets has expanded significantly with the advent of mRNA therapeutics. Scientists require exceptional accuracy and confidence to adequately characterize these therapeutics and their anticipated effects. To this end, Droplet Digital PCR (ddPCR) technology has proven invaluable. With unparalleled precision and absolute quantification, Droplet Digital PCR is revolutionizing the field of mRNA therapeutic development and manufacturing.

The post Achieving True Excellence in RNA Therapeutic Development first appeared on Bio-Radiations.]]> Exploring Circulating Tumor DNA (ctDNA) as a New Oncology Clinical Trial Endpoint https://www.bioradiations.com/exploring-ctdna-as-an-oncology-trial-endpoint-223/ Tue, 14 Feb 2023 18:52:58 +0000 https://www.bioradiations.com/?p=12281 Historically, five-year survival rates have been used to evaluate cancer therapeutic effectiveness in clinical trials. But time is critical for research — and for patients. Fortunately, there is a promising method for evaluating cancer therapeutic effectiveness that could potentially offer prognostic insights after just one year. Noninvasive liquid biopsy analysis, including analysis of circulating tumor DNA, is improving researchers’ ability to detect clinical responses more rapidly.

The post Exploring Circulating Tumor DNA (ctDNA) as a New Oncology Clinical Trial Endpoint first appeared on Bio-Radiations.]]> Maximizing the Impact of Wastewater-Based Epidemiology with ddPCR Technology https://www.bioradiations.com/maximizing-the-impact-of-wastewater-based-epidemiology-with-ddpcr-1022/ Tue, 11 Oct 2022 18:48:47 +0000 https://www.bioradiations.com/?p=12198 As SARS-CoV-2 becomes an endemic agent worldwide, communities and health agencies must figure out how to identify novel variants and track infection rates in ways that are sustainable, sensitive, and accurate. Fortunately, wastewater-based epidemiology (WBE) has proven its ability to play an important role in monitoring COVID-19 outbreaks, and Droplet Digital PCR (ddPCR) has become a cornerstone method for ongoing wastewater-based disease surveillance. In this article, we review the role of wastewater-based testing in community health before, during, and beyond the COVID-19 pandemic, and we explore why ddPCR technology is ideally suited for this application as it continues to expand and evolve.

The post Maximizing the Impact of Wastewater-Based Epidemiology with ddPCR Technology first appeared on Bio-Radiations.]]> Using ddPCR Technology to Improve Cancer Detection and Treatment Efficacy Monitoring https://www.bioradiations.com/using-ddpcr-to-improve-cancer-detection-1022/ Tue, 11 Oct 2022 18:35:03 +0000 https://www.bioradiations.com/?p=12232 Cancer remains a leading cause of death worldwide. However, as cancer treatments continue to improve and diagnoses happen sooner, more and more patients are experiencing remission. To further increase survival rates and reduce unnecessary treatment, scientists and clinicians are turning to ultra-sensitive molecular analysis to inform treatment decisions and predict and detect relapse.

The post Using ddPCR Technology to Improve Cancer Detection and Treatment Efficacy Monitoring first appeared on Bio-Radiations.]]> Precise Cell and Gene Therapy Potency Quantification https://www.bioradiations.com/precise-cell-and-gene-therapy-potency-quantification-722/ Wed, 13 Jul 2022 02:56:38 +0000 https://www.bioradiations.com/?p=12161 Inadequate quality control assays shouldn't limit cell and gene therapy applications. Instead, quantifying the potency of cell and gene therapies is essential for successful therapeutics. In this article, we describe multiple leading potency assays developed using Droplet Digital PCR (ddPCR) technology, including an assay that quantifies transgene integration and expression in one step.

The post Precise Cell and Gene Therapy Potency Quantification first appeared on Bio-Radiations.]]> Modernizing Gene Editing Detection with Droplet Digital PCR https://www.bioradiations.com/modernizing-gene-editing-detection-with-droplet-digital-pcr-722/ Wed, 13 Jul 2022 02:30:52 +0000 https://www.bioradiations.com/?p=12156 Gene editing is only useful if it’s successful. Read how scientists are leveraging the unparalleled accuracy and specificity of ddPCR technology to optimize quality control in gene editing projects, including HIV detection and CAR-T cell generation.

The post Modernizing Gene Editing Detection with Droplet Digital PCR first appeared on Bio-Radiations.]]> Trends in Wastewater-Based Epidemiology for 2022 https://www.bioradiations.com/trends-in-wastewater-based-epidemiology-for-2022-422/ Tue, 12 Apr 2022 16:29:04 +0000 https://www.bioradiations.com/?p=12108 Two years into the COVID-19 pandemic, trends point to a transition for the SARS-CoV-2 virus into an endemic agent. As major surveillance infrastructure scales back, it will be critical to maintain a cost-effective, simple, and rapid strategy for detecting and monitoring the spread of new variants. The key to such a strategy? Wastewater, where all disease agents leave their unique signature.

The post Trends in Wastewater-Based Epidemiology for 2022 first appeared on Bio-Radiations.]]> Wastewater SARS-CoV-2 Testing with Droplet Digital PCR (ddPCR) Technology https://www.bioradiations.com/wastewater-sars-cov-2-testing-with-ddpcr-422/ Tue, 12 Apr 2022 16:20:44 +0000 https://www.bioradiations.com/?p=12111 Wastewater-based epidemiology indirectly monitors infection rates in a community based on pathogen levels in local wastewater. Because symptomatic and asymptomatic SARS-CoV-2 infected individuals shed viral RNA in sewer systems, analysis of SARS-CoV-2 in sewage networks can provide information about SARS-CoV-2 infections within the community. However, the accuracy of these data depends on the quality of the wastewater testing method. During the COVID-19 pandemic, Droplet Digital PCR has proven that it has the sensitivity needed to detect outbreaks and monitor SARS-CoV-2 variants in communities via wastewater testing.

The post Wastewater SARS-CoV-2 Testing with Droplet Digital PCR (ddPCR) Technology first appeared on Bio-Radiations.]]> Bispecific Antibodies and Cancer Immunotherapy https://www.bioradiations.com/bispecific-antibodies-and-cancer-immunotherapy-122/ Tue, 11 Jan 2022 08:59:13 +0000 https://www.bioradiations.com/?p=12024 Bispecific antibodies (bsAbs) are an important addition to the immuno-oncology toolbox. Designed to recognize two distinct epitopes, bsAbs have enhanced binding, specificity, and efficacy compared to current monovalent antibody therapeutics, making them exciting candidates for more targeted cancer treatments. Learn about bsAbs and how our tools can help scientists with their research on therapeutic candidates.

The post Bispecific Antibodies and Cancer Immunotherapy first appeared on Bio-Radiations.]]> Monitoring CAR T-Cell Therapy Quality with Droplet Digital PCR https://www.bioradiations.com/monitoring-car-t-cell-therapy-quality-with-droplet-digital-pcr-122/ Tue, 11 Jan 2022 08:58:38 +0000 https://www.bioradiations.com/?p=12028 The CAR T-cell manufacturing process involves high potential for variability. Learn how sensitive ddPCR assays can be used to ensure safety and efficacy.

The post Monitoring CAR T-Cell Therapy Quality with Droplet Digital PCR first appeared on Bio-Radiations.]]> CDMOs Drive Cell and Gene Therapy from Behind the Scenes https://www.bioradiations.com/cdmos-drive-cell-and-gene-therapy-from-behind-the-scenes-921/ Tue, 14 Sep 2021 16:35:41 +0000 https://www.bioradiations.com/?p=11959 As more of the cell and gene therapies revolutionizing healthcare are approved for use, innovator companies usually get the credit. But many therapies would never make it to market without the expertise and capabilities of contract development and manufacturing organizations (CDMOs) operating behind the scenes. Learn more about how CDMOs enable new cell and gene therapies to come to market, and how Droplet Digital PCR can help.

The post CDMOs Drive Cell and Gene Therapy from Behind the Scenes first appeared on Bio-Radiations.]]> Top 5 Takeaways from ddPCR World 2021 https://www.bioradiations.com/top-5-takeaways-from-ddpcr-world-2021/ Fri, 06 Aug 2021 18:53:29 +0000 https://www.bioradiations.com/?p=11918 Precision diagnostics for cancer. COVID-19 surveillance in wastewater. Quality control of cell and gene therapies. These were just a few of the cutting-edge applications on display at the Droplet Digital PCR (ddPCR) World 2021 symposium. Recapping the event in 5 takeaways, moderator Francisco Bizouarn shares how international experts are leveraging ddPCR technology to solve today’s scientific and public health problems.

The post Top 5 Takeaways from ddPCR World 2021 first appeared on Bio-Radiations.]]> Advancing Molecular Diagnostics for Myelodysplastic Syndrome https://www.bioradiations.com/advancing-molecular-diagnostics-for-myelodysplastic-syndrome/ Wed, 09 Jun 2021 01:03:30 +0000 https://www.bioradiations.com/?p=11885 No patient who has survived cancer wants to hear that they’ve relapsed. But hearing it sooner rather than later can dramatically increase the chances of finding a successful, potentially life-saving treatment. Using next-generation sequencing and Droplet Digital PCR, researchers at the University of Uppsala aim to detect relapse by monitoring patient-specific biomarkers. Read about their ongoing clinical study.

The post Advancing Molecular Diagnostics for Myelodysplastic Syndrome first appeared on Bio-Radiations.]]> With Cell and Gene Therapies, Success and Quality Control Go Hand-in-Hand https://www.bioradiations.com/with-cell-and-gene-therapies-success-and-quality-control-go-hand-in-hand/ Tue, 11 May 2021 17:57:30 +0000 https://www.bioradiations.com/?p=11856 While the FDA has approved Abecma, the first CAR-T cell therapy in its class, regulatory approval is just the beginning for cell and gene therapies. Such therapies' safety and financial viability rest on a robust manufacturing and quality control program with the flexibility to scale up and out. Droplet Digital PCR is a sensitive biopharmaceutical quality control tool, offering absolute quantification of nucleic acids and other molecules in a sample.

The post With Cell and Gene Therapies, Success and Quality Control Go Hand-in-Hand first appeared on Bio-Radiations.]]> The Role of ddPCR in Developing Safe and Effective AAV-Based Gene Therapies https://www.bioradiations.com/the-role-of-ddpcr-in-developing-safe-and-effective-aav-based-gene-therapies/ Mon, 08 Mar 2021 19:46:06 +0000 https://www.bioradiations.com/?p=11782 Developing adeno-associated virus (AAV)-mediated gene therapies requires accurate and precise quality control. Read how Droplet Digital PCR technology provides the sensitivity needed to assess the potential safety and effectiveness of these treatments.

The post The Role of ddPCR in Developing Safe and Effective AAV-Based Gene Therapies first appeared on Bio-Radiations.]]> Top Questions from Our Wastewater Surveillance Webinar https://www.bioradiations.com/top-questions-from-our-wastewater-surveillance-webinar/ Tue, 08 Dec 2020 18:52:32 +0000 https://www.bioradiations.com/?p=11754 The COVID-19 pandemic has captured the attention of the world and won’t let go. And that has sparked efforts for creative solutions in some surprising places. Wastewater is not where most peoples’ minds go first when they think of airborne diseases, but it holds much promise for tracing and predicting SARS-CoV-2 outbreaks.

The post Top Questions from Our Wastewater Surveillance Webinar first appeared on Bio-Radiations.]]> Top 5 Challenges Faced by AAV Developers Today https://www.bioradiations.com/top-5-challenges-faced-by-aav-developers-today/ Tue, 08 Dec 2020 18:49:01 +0000 https://www.bioradiations.com/?p=11732 Adeno-associated viruses (AAVs) are some of the most commonly used viral vectors for gene therapy, supporting approximately 250 clinical trials to date. However, the manufacturing processes are challenging to scale. Read about five key issues that gene therapy manufacturers who use AAVs need to look out for.

The post Top 5 Challenges Faced by AAV Developers Today first appeared on Bio-Radiations.]]> A New International Standard to Measure DNA? https://www.bioradiations.com/a-new-international-standard-to-measure-dna/ Wed, 09 Sep 2020 22:36:43 +0000 https://www.bioradiations.com/?p=11665 From quantifying a patient’s tumor DNA to counting the viral load of an individual infected with COVID-19, the breadth of digital PCR (dPCR) applications continues to grow and fulfill critical scientific needs. dPCR offers the ability to count nucleic acid molecules directly, rather than extrapolating the quantity from standard curves as with many other methods. By harmonizing diagnostic procedures on dPCR platforms, the scientific and medical communities can become more coordinated and efficient.

The post A New International Standard to Measure DNA? first appeared on Bio-Radiations.]]> Expert Coffee Chats: Coffee Chat #4 – Droplet Digital PCR in Cell and Gene Therapy https://www.bioradiations.com/expert-coffee-chats-coffee-chat-4-droplet-digital-pcr-in-cell-and-gene-therapy/ Wed, 10 Jun 2020 17:41:19 +0000 https://www.bioradiations.com/?p=11564 Presented by: Matthew L. Turner, PhD, Field Application Scientist - ddPCR, NGC and qPCR, Tara Ellison, PhD, Senior Field Application Scientist

Date: June 17, 2020
Time: 9 AM, U.S. Pacific time

  • Robust methodology for viral titer using ddPCR
  • Precise copy number determination & improved confidence in acceptance criteria
  • Enhance your throughput and minimize hands-on time
  • Learn how ddPCR is used in other key applications
The post Expert Coffee Chats: Coffee Chat #4 – Droplet Digital PCR in Cell and Gene Therapy first appeared on Bio-Radiations.]]> Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies https://www.bioradiations.com/expanded-droplet-digital-pcr-multiplexing-capability-using-two-different-strategies/ Wed, 11 Sep 2019 17:38:33 +0000 https://www.bioradiations.com/?p=11221 Multiplexing with Droplet Digital PCR (ddPCR) allows for improved sensitivity and precision, as well as increased low-level detection in limited sample types like liquid biopsies. Learn about amplitude and probe-mixing multiplexing and how our ddPCR Assays can help set you up for multiplexing success.

The post Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies first appeared on Bio-Radiations.]]> Rapid, Sensitive Cancer Biomarker Screening in cfDNA https://www.bioradiations.com/rapid-sensitive-cancer-biomarker-screening-in-cfdna/ Fri, 14 Apr 2017 00:32:06 +0000 http://www.bioradiations.com/?p=9094 Disease-associated mutations can be prognostic of outcome and inform treatment choices. However, traditional tissue biopsies limit the ability to implement personalized treatments. Read about how multiplex digital PCR can enable the tracking of treatment efficacy using liquid biopsies.

The post Rapid, Sensitive Cancer Biomarker Screening in cfDNA first appeared on Bio-Radiations.]]> Digital PCR: Helpful Tips When Using Droplet Partitioning Technology https://www.bioradiations.com/digital-pcr-helpful-tips-when-using-droplet-partitioning-technology/ Thu, 10 Nov 2016 00:43:28 +0000 http://www.bioradiations.com/?p=8907 Digital PCR offers a highly sensitive alternative to conventional qPCR, enabling absolute quantification of nucleic acids and rare allele detection. The tips in this article help you set up and optimize your digital PCR experiments when using droplet partitioning technology.

The post Digital PCR: Helpful Tips When Using Droplet Partitioning Technology first appeared on Bio-Radiations.]]> Research Review: Phasing Genomes using Droplet Digital™ PCR https://www.bioradiations.com/research-review-phasing-genomes-using-droplet-digital-pcr/ Thu, 16 Jun 2016 22:05:29 +0000 http://www.bioradiations.com/?p=8659 Find out how a new Droplet Digital PCR-based technique called Drop Phase lets you quickly and easily phase genomes to better understand diseases like cystic fibrosis.

The post Research Review: Phasing Genomes using
Droplet Digital™ PCR first appeared on Bio-Radiations.]]> A Semi-Automated Quantitative PCR Approach to Assess Differential Anti-Apoptotic Gene Expression Patterns Associated with Prostate Cancer Progression https://www.bioradiations.com/a-semi-automated-quantitative-pcr-approach-to-assess-differential-anti-apoptotic-gene-expression-patterns-associated-with-prostate-cancer-progression/ Wed, 14 Oct 2015 00:35:36 +0000 http://www.bioradiations.com/?p=7750 Gene expression analyses become complicated and extremely time consuming when large numbers of genes need to be analyzed. Here we present a semi-automated qPCR workflow with reduced setup and processing times using Bio-Rad's PrimePCR™ Panels and CFX Automation System II Plate Handler.

The post A Semi-Automated Quantitative PCR Approach to Assess Differential Anti-Apoptotic Gene Expression Patterns Associated with Prostate Cancer Progression first appeared on Bio-Radiations.]]> Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital PCR https://www.bioradiations.com/ultra-sensitive-quantification-of-genome-editing-events-using-droplet-digital-pcr/ Tue, 25 Aug 2015 18:52:06 +0000 http://www.bioradiations.com/?p=7437 Genome editing using tools such as CRISPR/Cas9 and TALEN has gained enormous popularity in recent days due to their ability to create precise deletions or insertions. However when the frequency of these gene edits are really small, an ultrasensitive method like ddPCR™ is needed for accurate quantification. This technote explains how Droplet Digital™ PCR is used for the detection of HDR and NHEJ alleles generated using CRISPR/Cas9 and TALEN editing systems.

The post Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital PCR first appeared on Bio-Radiations.]]> Using Droplet Digital™ PCR to Monitor Environmental Contaminants in Water https://www.bioradiations.com/using-droplet-digital-pcr-to-monitor-environmental-contaminants-in-water/ Wed, 15 Oct 2014 19:00:10 +0000 http://www.bioradiations.com/?p=4436 South of Los Angeles, near some of America’s most famous beaches, one small agency is working on new methods for coastal water monitoring. Watch this video and see how scientists from SCCWRP, the Southern California Coastal Water Research Project, are using Droplet Digital PCR to watch over swimmer safety and ensure a clean environment.

The post Using Droplet Digital™ PCR to Monitor Environmental Contaminants in Water first appeared on Bio-Radiations.]]> Protecting Coastal Ecosystems with Droplet Digital™ PCR https://www.bioradiations.com/protecting-coastal-ecosystems-with-droplet-digital-pcr/ Tue, 14 Oct 2014 19:00:24 +0000 http://www.bioradiations.com/?p=2025 Who’s watching over swimmer safety at America’s beaches? Go inside one agency tasked with pioneering new methods for water quality monitoring, and see how its scientists are employing Droplet Digital PCR technology to check coastal waters for harmful pathogens.

The post Protecting Coastal Ecosystems with Droplet Digital™ PCR first appeared on Bio-Radiations.]]> Genomic Variation Analysis with Droplet Digital PCR: Success Stories https://www.bioradiations.com/genomic-variation-analysis-with-droplet-digital-pcr-success-stories/ Tue, 10 Jun 2014 22:38:18 +0000 http://www.bioradiations.com/?p=1024 Researchers are having success applying Droplet Digital PCR (ddPCR™) technology across many different fields. Here are two such success stories, one involving the application of ddPCR in detecting mutations in iPS cells, the other in predicting allele-specific variation in human brain development.

The post Genomic Variation Analysis with Droplet Digital PCR: Success Stories first appeared on Bio-Radiations.]]> The Ever-Expanding Universe of Droplet Digital™ PCR Applications https://www.bioradiations.com/the-ever-expanding-universe-of-droplet-digital-pcr-applications/ Tue, 11 Mar 2014 21:24:19 +0000 http://www.bioradiations.com/?p=1597 Bio-Rad’s groundbreaking Droplet Digital™ PCR technology can be applied in a variety of applications to glean precious information that cannot be obtained by any other method. Find out more about how this technology has been used in applications such as quantification of cancer biomarkers, validation of next generation sequencing, distinguishing genomic variation, and more.

The post The Ever-Expanding Universe of Droplet Digital™ PCR Applications first appeared on Bio-Radiations.]]> Transforming Cancer Research: Droplet Digital PCR Plays Integral Role at Fred Hutchinson Cancer Research Center https://www.bioradiations.com/transforming-cancer-research-droplet-digital-pcr-plays-integral-role-at-fred-hutchinson-cancer-research-center/ Tue, 11 Mar 2014 20:11:22 +0000 http://www.bioradiations.com/?p=1008 Droplet Digital PCR has major implications for cancer studies, where the ability to accurately measure biomarkers may result in important insights into cancer genesis and progression. Find out about new findings on micro RNA and Tumor Infilitrating Lymphocytes made possible by ddPCR technology.

The post Transforming Cancer Research: Droplet Digital PCR Plays Integral Role at Fred Hutchinson Cancer Research Center first appeared on Bio-Radiations.]]> Breaking Leukemia’s Limits of Detection with Droplet Digital™ PCR https://www.bioradiations.com/breaking-leukemias-limits-of-detection-with-droplet-digital-pcr-video/ Mon, 16 Sep 2013 21:13:12 +0000 http://www.bioradiations.com/?p=2187 Prof. Alec Morley, a pioneer in digital PCR, describes his use of Droplet Digital PCR (ddPCR™) technology to advance the diagnosis and treatment of leukemia. Morley and his colleagues at Flinders University and Medical Center in Adelaide, South Australia previously developed a digital PCR method for the early detection of residual acute lymphoblastic leukemia (ALL). Learn how Morley is now using Bio-Rad's ddPCR technology to develop a diagnostic test for monitoring chronic myeloid leukemia.

The post Breaking Leukemia’s Limits of Detection with Droplet Digital™ PCR first appeared on Bio-Radiations.]]> Breaking Leukemia’s Limits of Detection with Droplet Digital PCR https://www.bioradiations.com/breaking-leukemias-limits-of-detection-with-droplet-digital-pcr/ Wed, 11 Sep 2013 05:11:54 +0000 http://www.bioradiations.com/?p=524 In the early 1990s, researcher Alec Morley and his colleagues pioneered digital PCR techniques to measure acute lymphoblastic leukemia. Today, Morley is trying to develop a more accurate way to quantify chronic myeloid leukemia. Find out why he chose Bio-Rad’s Droplet Digital PCR technology for this effort.

The post Breaking Leukemia’s Limits of Detection with Droplet Digital PCR first appeared on Bio-Radiations.]]> Digital PCR at the Next Level: Users Find Many Applications for the QX100™ Droplet Digital™ PCR System https://www.bioradiations.com/digital-pcr-at-the-next-level-users-find-many-applications-for-the-qx100-droplet-digital-pcr-system/ Tue, 12 Mar 2013 19:00:55 +0000 http://www.bioradiations.com/?p=2610 The QX100 Droplet Digital PCR system gives researchers an improvement in PCR precision and sensitivity. See how this innovative digital PCR system is enabling companies to create better products, improve workflow efficiencies, and validate results with greater accuracy than previous methods allowed.

The post Digital PCR at the Next Level: Users Find Many Applications for the QX100™ Droplet Digital™ PCR System first appeared on Bio-Radiations.]]> QX100™ Droplet Digital™ ddPCR™ System at Sangamo BioSciences https://www.bioradiations.com/qx100-droplet-digital-ddpcr-system-at-sangamo-biosciences/ Fri, 13 Jul 2012 22:36:02 +0000 http://www.bioradiations.com/?p=2250 Researchers at Sangamo BioSciences, Inc. explain the benefits of using third generation PCR as it applies to their research in the field of personalized medicine. Watch to see the latest generation of quantitative PCR in action.

The post QX100™ Droplet Digital™ ddPCR™ System at Sangamo BioSciences first appeared on Bio-Radiations.]]> Droplet Digital PCR Opens New Perspectives in HIV Research https://www.bioradiations.com/droplet-digital-pcr-opens-new-perspectives-in-hiv-research/ Fri, 15 Jun 2012 22:42:23 +0000 http://www.bioradiations.com/?p=1040 Sangamo BioSciences, Inc., located in Richmond, California, is developing HIV therapeutics toward a functional cure using their proprietary zinc finger DNA-binding protein technology. Droplet digital PCR (ddPCR™) — or third generation PCR — offers a superior solution as it can detect target sequence within a single molecule, making it a powerful technology for low copy event detection.

The post Droplet Digital PCR Opens New Perspectives in HIV Research first appeared on Bio-Radiations.]]> The QX100™ Droplet Digital™ PCR System Workflow https://www.bioradiations.com/the-qx100-droplet-digital-pcr-system-workflow/ Mon, 16 Apr 2012 19:00:10 +0000 http://www.bioradiations.com/?p=2256 Watch the step-by-step workflow for absolute quantification of DNA molecules using the third generation droplet digital PCR technology.

The post The QX100™ Droplet Digital™ PCR System Workflow first appeared on Bio-Radiations.]]> A Simple Method for Obtaining Absolute Quantification of DNA Molecules Using the Innovative Droplet Digital™ PCR Technology https://www.bioradiations.com/a-simple-method-for-obtaining-absolute-quantification-of-dna-molecules-using-the-innovative-droplet-digital-pcr-technology/ Thu, 29 Mar 2012 19:18:12 +0000 http://www.bioradiations.com/?p=3484 Droplet digital PCR is considered the third generation of PCR technology. This technology enables the quantification of absolute measure of target DNA molecules, rather than the relative measure obtained from real-time PCR. Absolute quantification is advantageous in several applications, such as measuring copy number variation, detecting rare sequences and mutations, and analyzing gene expression.

The post A Simple Method for Obtaining Absolute Quantification of DNA Molecules Using the Innovative Droplet Digital™ PCR Technology first appeared on Bio-Radiations.]]> A New Paradigm for Precise Quantitation of RNA https://www.bioradiations.com/a-new-paradigm-for-precise-quantitation-of-rna/ Mon, 27 Feb 2012 20:31:17 +0000 http://www.bioradiations.com/?p=4200 The one-step RT-ddPCR kit for probes creates a new paradigm for the precise quantitation of RNA by combining reverse transcription with Droplet Digital™ PCR (ddPCR™), providing an absolute measure of target RNA molecules with unrivaled precision and sensitivity

The post A New Paradigm for Precise Quantitation of RNA first appeared on Bio-Radiations.]]>