Cell Biology | Bio-Radiations

Build Successful Panels for Spectral Flow Cytometry with These Seven Tips

Bioradiations Staff — Mon, 09 Feb 2026 17:47:53 +0000

IT’S HERE! The core lab finally invested in a spectral flow cytometer, and you can now combine your two, 20-color panels into one large panel, potentially reducing instrument time by half. However, to build the optimal panel it is important to consider where to begin ...

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10 Tips for Optimizing High-Throughput Flow Cytometry Assays

Bioradiations Staff — Tue, 11 Nov 2025 17:55:05 +0000

Flow cytometry can be labor intensive, and efforts to increase throughput can lead to reduced data quality or even instrument reliability issues such as blockages. While some instruments are more ...

The post 10 Tips for Optimizing High-Throughput Flow Cytometry Assays first appeared on Bio-Radiations.

Optimize Your Assays for High-Throughput Flow Cytometry

Bioradiations Staff — Tue, 11 Nov 2025 17:53:11 +0000

Flow cytometry is a powerful technique with many applications in both academia and drug discovery. It can facilitate complex immunophenotyping experiments, making it an indispensable tool for understanding biological systems. ...

The post Optimize Your Assays for High-Throughput Flow Cytometry first appeared on Bio-Radiations.

Vive la Différence: The Beauty of Diversity in Life, Life Science, and Flow Cytometry

Bioradiations Staff — Tue, 11 Feb 2025 19:23:13 +0000

The differences between us are what makes life interesting, and diversity in a group that shares a common goal can lead to many benefits. Here we examine the similarities and differences between researchers using flow cytometry for academic research and those using it for drug discovery. We outline the challenges they face and learn that they share a common goal as well as a common solution.

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Biotherapeutic Antibody Discovery at Bio-Rad’s New Center for Excellence

Bioradiations Staff — Tue, 11 Feb 2025 18:19:21 +0000

The Pioneer Platform features one of the largest functional antibody libraries available, utilizing SpyLock technology for rapid prototyping and screening of hundreds of bispecific antibodies, and TrailBlazer technology for efficient antibody format conversion. Together, these services provide a comprehensive and streamlined solution for researchers seeking to develop innovative biotherapeutic therapies.

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Cytometry in Drug Discovery: An Introduction to Regulation, Standardization, and Productivity

Bioradiations Staff — Tue, 11 Feb 2025 17:40:52 +0000

Many researchers learn flow cytometry in academia, but find new challenges in the commercial sector. In this webinar, learn about regulatory issues and more.

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10 Key Factors for Achieving Accurate Cell Counts: Tips, Tricks, and Myths

Bioradiations Staff — Tue, 12 Nov 2024 17:47:36 +0000

Counting is something we are all familiar with but it’s not always as simple as one, two, three. Measuring the concentration of cells in suspension is a common but surprisingly complex task in cell biology that is easy to get wrong. This article explores ten factors that can influence cell counting, gives advice on how to improve accuracy, and dispels some commonly held myths.

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Faster PCR and Flow Cytometry Highlighted in Annual Lab Automation Roundup

Bioradiations Staff — Tue, 13 Aug 2024 19:53:00 +0000

The annual international Society for Laboratory Automation and Screening (SLAS) conference is the highlight of the year for scientists in academia, industry, and government focused on leveraging the power of automation to achieve scientific breakthroughs. Here are some highlights of this year’s conference and how Bio-Rad contributed to it.

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Flow Cytometry and the Law of Bottlenecks

Bioradiations Staff — Tue, 11 Jun 2024 19:13:40 +0000

Manufacturing and high-throughput screening may seem to have very little in common, but they are related at a fundamental level. Both seek to transform a raw material into a finished product, and both struggle with constraints, or bottlenecks, that reduce efficiency. How can we apply the revolutionary 20th century management principles of continuous improvement to 21st century flow cytometry?

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Rising from the Ashes: The History of the 488 nm Laser

Bioradiations Staff — Tue, 11 Jun 2024 19:04:42 +0000

Despite the pivotal role of the 488 nm (blue) laser in the rise in popularity of flow cytometry, it experienced somewhat of a fall from grace following the shift to higher usage of the 561 nm (yellow) laser. However, with the advancement of flow cytometry leading to the use of more complex multicolor panels and ever-increasing fluorophore numbers per experiment, the blue laser is starting to make a comeback. Discover how StarBright Blue Dyes can help you make the most out of your 488 nm laser.

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StarBright Dyes for Flow Cytometry

Bioradiations Staff — Mon, 12 Feb 2024 18:11:40 +0000

Watch this video to learn about the many benefits of StarBright Dyes — our proprietary fluorescent nanoparticles — and see how they can help you build bigger, better flow cytometry panels with ease. StarBright Dyes have been designed to be stable, with superior brightness, narrow excitation and emission characteristics, and the flexibility to be included in new and existing experiments. They offer improved resolution of specific cell populations and minimized spillover and spreading.

The post StarBright Dyes for Flow Cytometry first appeared on Bio-Radiations.

Developing Biotherapeutics for Lung Cancer Treatment

Bioradiations Staff — Mon, 12 Feb 2024 18:07:52 +0000

In recent years, biologics — drugs derived from living culture systems — have become increasingly significant in the fight against a plethora of conditions. Unfortunately, the efficiency of these drugs comes at a cost. A daily dose of a biologic drug costs, on average, 22 times more than conventional small-molecule drugs. Therefore, biosimilars are developed to make them more accessible. Lung cancer is an example where the development of biosimilars could increase treatment options available for patients.

The post Developing Biotherapeutics for Lung Cancer Treatment first appeared on Bio-Radiations.

The Pioneer Antibody Discovery Platform: Clinical-Quality Lead Candidates in Industry-Leading Timelines

Bioradiations Staff — Tue, 14 Nov 2023 19:51:08 +0000

Biotherapeutic antibody discovery is inherently challenging. Amongst the clinically proven technologies for antibody discovery is phage display — a technique utilized by one of the most recent and innovative solutions on the market: the Pioneer Antibody Discovery Platform.

The post The Pioneer Antibody Discovery Platform: Clinical-Quality Lead Candidates in Industry-Leading Timelines first appeared on Bio-Radiations.

Efficacy of a Novel Treatment for Multiple Myeloma Established by Flow Cytometry

Bioradiations Staff — Tue, 14 Nov 2023 19:39:59 +0000

Autologous stem cell transplantation (ASCT) is an effective treatment strategy for multiple myeloma (MM) but isolating enough hematopoietic stem and progenitor cells (HSPCs) for transplantation can be challenging. The selective CXCR4 inhibitor motixafortide has been shown to be a safe and efficacious HSPC mobilization agent in a phase 2 trial when paired with G-CSF. Crees et al. (2023) report the results of the phase 3 trial, in which they used the ZE5 Cell Analyzer to explore motixafortide’s mobilizing effects.

The post Efficacy of a Novel Treatment for Multiple Myeloma Established by Flow Cytometry first appeared on Bio-Radiations.

In-Cell Western: A Versatile Tool for Quantitative Analysis of Protein Expression In-Situ

Bioradiations Staff — Mon, 07 Aug 2023 20:38:44 +0000

Protein expression analysis is essential in cell biology research, as it provides valuable insights into the molecular mechanisms that underlie cellular functions. Traditional methods for protein analysis, such as western blotting and ELISA, are useful for studying protein expression in cell lysates. However, these methods require labor-intensive sample preparation and do not provide information on the spatial distribution of proteins within intact cells. In-Cell Western is a powerful technique that enables quantitative and qualitative in situ protein expression analysis.

The post In-Cell Western: A Versatile Tool for Quantitative Analysis of Protein Expression In-Situ first appeared on Bio-Radiations.

How to Build Bigger Immunophenotyping Panels with StarBright Dyes

Bioradiations Staff — Wed, 10 May 2023 08:20:05 +0000

With the advancement of technology, flow cytometers are now able to detect a much greater range of fluorescent dyes, facilitating the analysis of more extensive and complex multicolor immunophenotyping assays. But this has also introduced more pitfalls to navigate during the design of these experiments. Find out how StarBright Dyes can help circumvent many of these issues, enabling consistent and reliable results.

The post How to Build Bigger Immunophenotyping Panels with StarBright Dyes first appeared on Bio-Radiations.

Flow Cytometry Empowers CAR T-Cell Therapies

Bioradiations Staff — Tue, 14 Feb 2023 21:26:07 +0000

Chimeric antigen receptor (CAR) T cells represent the next generation of therapeutic interventions and are a major advancement in personalized disease treatment. CAR T-cell therapies utilize a patient’s own T cells to recognize and destroy cancer cells or other disease-causing cells. Find out how the ZE5 Cell Analyzer has helped overcome some of the challenges associated with development of these powerful new therapies.

The post Flow Cytometry Empowers CAR T-Cell Therapies first appeared on Bio-Radiations.

Leading the Way in Our Understanding of COVID-19: Peptide Megapool Assays

Bioradiations Staff — Wed, 13 Jul 2022 03:50:59 +0000

Understanding how T cells respond to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical in developing long-term therapeutic approaches, as well as helping inform strategies for future pandemics. Learn how one team at the La Jolla Institute for Immunology in San Diego, California, has been using novel T-cell activation assays to understand how our immune system responds to SARS-CoV-2.

The post Leading the Way in Our Understanding of COVID-19: Peptide Megapool Assays first appeared on Bio-Radiations.

The PI3K-AKT Pathway and Its Role in Tumorigenesis

Bioradiations Staff — Tue, 12 Apr 2022 16:21:43 +0000

The phosphoinositide 3-kinase/protein kinase B (PI3K-AKT) pathway is involved in the control of numerous critical cellular processes. PI3K-AKT pathway dysfunction has been observed in several diseases, including cancer, cardiovascular disease, and autoimmune and neurological disorders. In this animation, learn more about the molecular characteristics and function of the PI3K-AKT pathway and how AKT mutation plays a role in tumorigenesis.

The post The PI3K-AKT Pathway and Its Role in Tumorigenesis first appeared on Bio-Radiations.

The ZE5 Cell Analyzer, a Fast and Flexible Flow Cytometer for High-Throughput Screening

Bioradiations Staff — Tue, 12 Apr 2022 16:18:41 +0000

High-throughput screening (HTS) is a critical component of the entire drug discovery process. Flow cytometry is an attractive alternative to imaging and plate reader assays for HTS in drug discovery, due to its increased sensitivity and multiplexing capabilities. The ZE5 Cell Analyzer eliminates pain points that have hindered the full adoption of flow cytometry for drug discovery.

The post The ZE5 Cell Analyzer, a Fast and Flexible Flow Cytometer for High-Throughput Screening first appeared on Bio-Radiations.

5 Reasons to Read the Antibody Advice Guide

Bioradiations Staff — Tue, 11 Jan 2022 08:57:55 +0000

In the day-to-day pressures of a research environment, it is natural to want to generate important data as quickly as possible. However, there are real benefits in ensuring that the antibody you have picked is the best for your experiment and spending time aliquoting and titrating your antibodies. Bio-Rad recently launched a free Antibody Advice Guide to equip scientists with the knowledge to get the best out of their experiments with antibodies. Read on for five examples of valuable information that you will learn from the guide, helping you generate publication-ready data.

The post 5 Reasons to Read the Antibody Advice Guide first appeared on Bio-Radiations.

5 High-Throughput Screening Applications Using Flow Cytometry

Bioradiations Staff — Tue, 14 Sep 2021 16:36:30 +0000

For many scientists, high-throughput flow cytometry screens are associated with drug or compound libraries, but did you know that you can also apply this technique to study microorganisms, clinical melanoma samples, and even protein trafficking? Read on for five examples of the use of flow cytometry in diverse, high-throughput screening applications.

The post 5 High-Throughput Screening Applications Using Flow Cytometry first appeared on Bio-Radiations.

6 Tips to Secure Funding for Flow Cytometers

Bioradiations Staff — Wed, 09 Jun 2021 01:38:24 +0000

We asked core facilities staff and research scientists from the U.S. and Europe for their top tips for securing funding for large instrumentation, such as a cell analyzer or cell sorter. Here are the key questions you should consider while preparing your grant application to increase your chances of a successful outcome.

The post 6 Tips to Secure Funding for Flow Cytometers first appeared on Bio-Radiations.

Immune Surveillance in SARS-CoV-2 Vaccine Development

Bioradiations Staff — Mon, 08 Mar 2021 22:14:23 +0000

The ability to characterize the target of cell-mediated immune response is crucial during vaccine development. In studying the characterization of SARS-CoV-2 infection pathogenesis in humans, a research team identified neutralizing antibodies that provided context for vaccine antigen design. Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein–specific antibodies in a rapid, high-throughput manner.

The post Immune Surveillance in SARS-CoV-2 Vaccine Development first appeared on Bio-Radiations.

How FACS, Genetic Screens, and the HAP1 Cell Line Uncover the Biological Mechanisms of Cancer

Bioradiations Staff — Mon, 08 Mar 2021 20:17:49 +0000

Genetic screens are powerful tools for finding genes associated with a particular phenotype. They play an especially important role in cancer research and are leveraged to identify predictive biomarkers that can be used to improve treatment efficacy or trace the progression of genetic alterations that ultimately lead to disease. The HAP1 cancer cell line model and fluorescence-activated cell sorting (FACS) bring efficiency to the development of genetic screens that is making them a force to be reckoned with.

The post How FACS, Genetic Screens, and the HAP1 Cell Line Uncover the Biological Mechanisms of Cancer first appeared on Bio-Radiations.

7 Tips for Purchasing Your Next Cell Sorter

Bioradiations Staff — Wed, 09 Sep 2020 22:01:53 +0000

Presented here are some tips to help you avoid buyer’s remorse and make an educated decision about which instrument to purchase. These tips provide you with the tools to make an informed decision when purchasing a cell sorter.

The post 7 Tips for Purchasing Your Next Cell Sorter first appeared on Bio-Radiations.

Sorting an Old Problem: Are Cells Stressed by Fluorescence-Activated Cell Sorting?

Bioradiations Staff — Wed, 09 Sep 2020 21:16:22 +0000

Fluorescence-activated cell sorting is a valuable tool for research, but could the physical forces of sorting stress the cells, altering their behavior and confounding experimental results? Recent research found that although cell sorting activated p38 MAPK stress signaling, no functional or structural changes were observable. The results indicate that jet-in-air systems such as the Bio-Rad S3e Cell Sorter do not alter cell behavior.

The post Sorting an Old Problem: Are Cells Stressed by Fluorescence-Activated Cell Sorting? first appeared on Bio-Radiations.

10 Tips for Setting Up a Successful Cell Sorting Experiment

Bioradiations Staff — Wed, 10 Jun 2020 17:59:14 +0000

Incorporating fluorescence-based cell sorting into your lab grants you powerful access to new research areas and rarer cell types without sacrificing efficiency or results. New, powerful techniques like CRISPR and single-cell analysis are enhanced by the isolation of specific cells. But isolating cell populations of the right quality and quantity is both challenging and time consuming. We offer some tips to set yourself up for success and get the most out of your sorts.

The post 10 Tips for Setting Up a Successful Cell Sorting Experiment first appeared on Bio-Radiations.

Improving CRISPR-Cas9 Gene Editing:Tackling the HDR Inefficiency Problem

Bioradiations Staff — Thu, 12 Mar 2020 00:04:27 +0000

A recent publication showed that expression of a protein involved in homology-directed repair (HDR) can improve efficiency of CRISPR-Cas9 gene editing. Learn how manipulation of DNA repair pathways and improvements to gene editing workflows, such as the addition of cell sorting, can facilitate applications of CRISPR-Cas9 technology.

The post Improving CRISPR-Cas9 Gene Editing:
Tackling the HDR Inefficiency Problem first appeared on Bio-Radiations.

GMP-Compliant Production of CAR-T Cells Using mRNA Transfection

Bioradiations Staff — Wed, 15 Jan 2020 22:43:59 +0000

Many patients with treatment-resistant blood cancers have found new hope in chimeric antigen receptor (CAR)-T cell therapy. But generation of CAR-T cells at clinical scale can be challenging. Find out how the Gene Pulser Xcell Electroporation System can be used to reduce costs and risks associated with clinical-scale CAR-T cell production, while maintaining GMP compliance.

The post GMP-Compliant Production of CAR-T Cells Using mRNA Transfection first appeared on Bio-Radiations.

Turning Back the Clock on Mouse Neural Progenitor Cells

Bioradiations Staff — Wed, 15 Jan 2020 22:25:36 +0000

Is the fate of an embryonic cell determined by the age of the cell or the age of the embryo? To explore this question, researchers isolated and transplanted live neural cells from mouse embryos at different stages of development. Learn about their findings and how the S3e Cell Sorter helped achieve this incredible feat.

The post Turning Back the Clock on Mouse Neural Progenitor Cells first appeared on Bio-Radiations.

Tyrosine Phosphorylation of EGF R

Bioradiations Staff — Tue, 13 Aug 2019 20:57:17 +0000

Growth factors are protein signaling molecules that play crucial roles in the regulation of numerous cellular processes such as proliferation, differentiation, and survival. Learn more about the phosphorylation of one such factor, the epidermal growth factor receptor (EGF R).

The post Tyrosine Phosphorylation of EGF R first appeared on Bio-Radiations.

Discover Our Capabilities

Bioradiations Staff — Wed, 10 Apr 2019 18:16:19 +0000

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.

The post Discover Our Capabilities first appeared on Bio-Radiations.

A 16-Color Flow Cytometry Panel to Monitor Checkpoint Inhibitor Expression and T-Cell Exhaustion Following Treatment with the PD1 Inhibitor Nivolumab

Bioradiations Staff — Tue, 09 Oct 2018 23:15:05 +0000

Presented by: Angie Green, PhD, Senior Scientist

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We discuss using a multiparameter flow cytometry panel to monitor T-cell exhaustion states and the expression of checkpoint inhibitors. We used an in vitro model; however, the panel is designed to be compatible with clinical samples to monitor immunotherapy.

The post A 16-Color Flow Cytometry Panel to Monitor Checkpoint Inhibitor Expression and T-Cell Exhaustion Following Treatment with the PD1 Inhibitor Nivolumab first appeared on Bio-Radiations.

R-Loops and Their Key Role in Cancer Research

Bioradiations Staff — Tue, 11 Sep 2018 19:11:59 +0000

R-loops are being investigated as potential therapeutic targets for cancer. Find out what they are, how they form, how their formation is controlled by our cells, and, above all, their potential as therapeutic targets.

The post R-Loops and Their Key Role in Cancer Research first appeared on Bio-Radiations.

To Boldly Flow: Gain Confidence to Start Flow Cytometry

Bioradiations Staff — Tue, 11 Sep 2018 18:56:01 +0000

Presented by: Mike Blundell, PhD, Product Manager

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Flow cytometry is a powerful technique used widely in biological research, but if you are just beginning your flow journey, it can be hard to know where to start. In this webinar, you will:

Learn the important steps to obtaining better flow cytometry data
Get advice on how to apply this knowledge to improve your experiments
Find out how Bio-Rad’s panel builder tool can help you design your experiments, no matter how many colors you plan to use

The post To Boldly Flow: Gain Confidence to Start Flow Cytometry first appeared on Bio-Radiations.

Flow Cytometry — A Gateway for Discovering More about Small Vesicles

Bioradiations Staff — Wed, 15 Aug 2018 18:42:00 +0000

Small vesicles were identified over 50 years ago but scientists are still trying to understand their biology and role in disease. These vesicles have become relevant for monitoring, diagnosing, and treating complex diseases such as cancer. Yet, because of their small size compared to cells, they require intricate technologies to truly understand their impact in research. Learn about the various technologies used to study small vesicles and discover the power of flow cytometry for learning more about these valuable vesicles.

The post Flow Cytometry — A Gateway for Discovering More about Small Vesicles first appeared on Bio-Radiations.

Finding a Good Antibody: Part III – Publish Meaningful Data

Bioradiations Staff — Tue, 10 Jul 2018 21:30:26 +0000

In the first two articles in our Finding a Good Antibody series, you learned how to select the best antibodies and received tips on proper antibody validation. In this third and final article, see how you can use this information to publish meaningful data when using antibodies. And get some guidelines for antibody best practices.

The post Finding a Good Antibody:
Part III – Publish Meaningful Data first appeared on Bio-Radiations.

The Benefits of Flow Cytometry in Immuno-Oncology Research

Bioradiations Staff — Tue, 12 Jun 2018 23:00:16 +0000

Immuno-oncology, or cancer immunotherapy, research focuses heavily on the various types of cells and their behavior in cancer, so the use of flow cytometry to interrogate these cells is a no brainer. See how flow cytometry fits in this growing field of research and how it can help identify new targets and improve drug design to generate more effective and safer cancer therapeutics.

The post The Benefits of Flow Cytometry in Immuno-Oncology Research first appeared on Bio-Radiations.

Finding a Good Antibody:Part II — Antibody Validation

Bioradiations Staff — Tue, 12 Jun 2018 22:41:34 +0000

Finding antibodies that work for your application is just the first step in finding a good antibody. Next is antibody validation — making sure that your antibody does what you think it is supposed to. In this second article in our Finding a Good Antibody series, learn how best to go about validating your antibodies.

The post Finding a Good Antibody:
Part II — Antibody Validation first appeared on Bio-Radiations.

When Small Matters — See How the ZE5 Cell Analyzer Can Help

Bioradiations Staff — Tue, 12 Jun 2018 22:40:04 +0000

Presented by: Yasha Talaga, MSc, Applications and Collaborations Product Manager, Cell Biology

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In this webinar, Yasha Talaga will explain how specific methodologies and instrument setup on the ZE5 Cell Analyzer allow for reliable exosome detection data.

The post When Small Matters — See How the ZE5 Cell Analyzer Can Help first appeared on Bio-Radiations.

Finding a Good Antibody:Part I — Selecting the Best Antibodies

Bioradiations Staff — Wed, 09 May 2018 19:21:26 +0000

Even though antibodies are central to basic research as well as drug development and diagnostics, concerns with antibody validation remain high, and finding an antibody that works well for a specific application can be quite a challenge. Use these tips to select the best antibodies for your application.

The post Finding a Good Antibody:
Part I — Selecting the Best Antibodies first appeared on Bio-Radiations.

Improve the Quality of Your Cell Analysis with the ZE5™ Cell Analyzer

Bioradiations Staff — Wed, 11 Apr 2018 19:57:40 +0000

Cell analysis is a crucial first step in many experiments so a reliable, flexible, and quick cell analyzer that produces high-resolution, reproducible results is what every lab needs. See how the ZE5™ Cell Analyzer can help improve the quality of your cell analysis experiments, instill confidence in your results, and shorten the time to publish.

The post Improve the Quality of Your Cell Analysis with the ZE5™ Cell Analyzer first appeared on Bio-Radiations.

Identifying Predictive Biomarkers for Successful Immunotherapy Treatments

Bioradiations Staff — Tue, 09 Jan 2018 23:08:16 +0000

Find out how the S3e™ Cell Sorter can help scientists discover new biomarkers that have the potential to become new therapies for cancer and other diseases.

The post Identifying Predictive Biomarkers for Successful Immunotherapy Treatments first appeared on Bio-Radiations.

Cell Sorting and Its Significance in Stem Cell Research

Bioradiations Staff — Thu, 16 Nov 2017 16:49:52 +0000

Stem cells possess the remarkable potential to self-renew and to differentiate into many different types of cells. Due to their unique ability to regenerate, they are being used in regenerative medicine and offer great hope for treating diseases such as diabetes and heart disease. However, since stem cells represent a rare population of cells, they are not always easy to study. See how the simplicity, efficiency, and flexibility of cell sorting is making it possible to isolate pure populations of stem cells for downstream analyses.

The post Cell Sorting and Its Significance in Stem Cell Research first appeared on Bio-Radiations.

Speed Time to Results by Incorporating Flow Cytometry into the CRISPR‑Cas9 Gene Editing Workflow

Bioradiations Staff — Tue, 12 Sep 2017 22:38:32 +0000

The power to edit a gene is the power to change its function, and with it the biology of a cell. From generating novel cell lines and better animal models for the discovery and preclinical phases of therapeutic research to actually creating a therapeutic itself, CRISPR gene editing is allowing science to advance rapidly. See how enrichment and cell sorting can help generate edited cells faster and more reliably.

The post Speed Time to Results by Incorporating Flow Cytometry into the CRISPR‑Cas9 Gene Editing Workflow first appeared on Bio-Radiations.

BrdU in Adult Neurogenesis Research

Bioradiations Staff — Wed, 09 Aug 2017 17:55:25 +0000

In this podcast, you will learn about the history of bromodeoxyuridine (BrdU) and how it was established as a popular tool for neurogenesis research. Gain insight into the latest BrdU-related findings and get five practical tips for controlling your BrdU labeling experiments.

The post BrdU in Adult Neurogenesis Research first appeared on Bio-Radiations.

Understanding the Eukaryotic Cell Cycle

Bioradiations Staff — Wed, 12 Jul 2017 21:20:57 +0000

The eukaryotic cell cycle is an evolutionarily conserved process that results in the replication of cells. Read this mini review to better understand the eukaryotic cell cycle and get an overview of common molecular techniques used to evaluate proliferating cells.

The post Understanding the Eukaryotic Cell Cycle first appeared on Bio-Radiations.

Assessing Cell Health: Necroptosis

Bioradiations Staff — Wed, 15 Mar 2017 23:05:29 +0000

In this fourth and final part of the series on assessing the health of your cells, we review the different stages of necroptosis, see how it differs from apoptosis, and learn about the various methods of assessing cellular necroptosis.

The post Assessing Cell Health: Necroptosis first appeared on Bio-Radiations.

The ZE5™ Cell Analyzer: Designed by Users for Users

Bioradiations Staff — Wed, 15 Feb 2017 23:00:07 +0000

Bio-Rad’s new ZE5 Cell Analyzer is a game-changing cell analyzer that was designed with direct input from scientists and flow core labs. Karen Helm runs the flow core facility at the University of Colorado Cancer Center and was one of the experts consulted. See how ZE5 is helping her flow core users do better science.

The post The ZE5™ Cell Analyzer: Designed by Users for Users first appeared on Bio-Radiations.

Assessing Cell Health: Autophagy

Bioradiations Staff — Thu, 13 Oct 2016 17:40:47 +0000

Part III of our cell health series describes the three main autophagy mechanisms and reviews the various methods for assessing macroautophagy and autophagic flux in cells.

The post Assessing Cell Health: Autophagy first appeared on Bio-Radiations.

Cleaning the Nozzle Tip on the S3e™ Cell Sorter

Bioradiations Staff — Thu, 15 Sep 2016 23:20:54 +0000

In this short video, we show how to clean the nozzle tip on the S3e Cell Sorter.

The post Cleaning the Nozzle Tip on the S3e™ Cell Sorter first appeared on Bio-Radiations.

Monocytes — a Mini Review

Bioradiations Staff — Thu, 15 Sep 2016 23:18:26 +0000

Monocytes play key roles in mediating innate and adaptive immune responses. Here is an overview of the types, functions, process of recruitment, and the role of monocytes in disease.

The post Monocytes — a Mini Review first appeared on Bio-Radiations.

Performing a Fluidics Hot Swap on the S3e™ Cell Sorter

Bioradiations Staff — Thu, 15 Sep 2016 23:13:47 +0000

In this video, we show how a fluidics hot swap can be performed without shutting down the S3e Cell Sorter.

The post Performing a Fluidics Hot Swap on the S3e™ Cell Sorter first appeared on Bio-Radiations.

Cleaning the Streams Camera Window on the S3e™ Cell Sorter

Bioradiations Staff — Thu, 15 Sep 2016 23:10:43 +0000

In this video we show you how to clean the camera streams window as part of routine maintenance for your S3e Cell Sorter.

The post Cleaning the Streams Camera Window on the S3e™ Cell Sorter first appeared on Bio-Radiations.

Assessing Cell Health: Apoptosis

Bioradiations Staff — Fri, 15 Jul 2016 21:30:37 +0000

In this second part of the series on assessing the health of your cells, we review the different types and stages of apoptosis, and the various methods of assessing apoptosis of cells.

The post Assessing Cell Health: Apoptosis first appeared on Bio-Radiations.

Macrophage Polarization — An Overview

Bioradiations Staff — Fri, 15 Jul 2016 21:28:12 +0000

Macrophages are important players in the phagocytosis process in the immune system. In this detailed review, we provide an overview on the different types of macrophages, the signaling molecules involved in the polarization of M1 and M2 macrophage subsets, and more.

The post Macrophage Polarization — An Overview first appeared on Bio-Radiations.

Assessing Cell Health: Viability and Proliferation

Bioradiations Staff — Thu, 16 Jun 2016 23:16:59 +0000

In this multi-part series on assessing the health of your cells, we review different factors to be considered in your assessments such as cell viability, proliferation, apoptosis, and autophagy. The first part covers the methods for assessing viability and proliferation and their advantages, and discusses some common considerations.

The post Assessing Cell Health: Viability and Proliferation first appeared on Bio-Radiations.

Expanding Flow Core Capabilities with the S3e™ Cell Sorter

Bioradiations Staff — Wed, 13 Apr 2016 01:00:38 +0000

Andy Riddell, Flow Cytometry Core Facility Manager at the Wellcome Trust- MRC Stem Cell Institutes, is expanding his core facility by allowing his researchers to conduct sorting on their own. In doing so, he is now free to explore and answer the challenging flow cytometry questions that require more of his expert time. Find out how the S3e Cell Sorter has allowed Andy that freedom and how easy sorting can be for researchers.

The post Expanding Flow Core Capabilities with the S3e™ Cell Sorter first appeared on Bio-Radiations.

Expanding Flow Core Capabilities by Shifting Routine Sorts to Self-Sort on the S3e™ Cell Sorter

Bioradiations Staff — Tue, 12 Apr 2016 20:50:48 +0000

To run a core flow cytometry lab is no easy task. There is always a balance between running simple sorts and developing new assays for answering complicated questions that demand pushing the limits of existing assays and the limitations of the sorters. See how Andy Riddell, a flow core manager, pushes the limits of his existing assays and how Bio-Rad’s S3e™ cell sorter is allowing him to accomplish that.

The post Expanding Flow Core Capabilities by Shifting Routine Sorts to Self-Sort on the S3e™ Cell Sorter first appeared on Bio-Radiations.

Flow Cytometry: Basic Definitions

Bioradiations Staff — Tue, 08 Dec 2015 19:07:09 +0000

It’s always good to get the basics straight. Here, we’ve defined some flow cytometry terms that might have eluded you.

The post Flow Cytometry: Basic Definitions first appeared on Bio-Radiations.

10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels

Bioradiations Staff — Tue, 10 Nov 2015 22:30:16 +0000

Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.

The post 10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels first appeared on Bio-Radiations.

Protocol: Preparation of Cells for Flow Cytometry

Bioradiations Staff — Wed, 14 Oct 2015 00:34:44 +0000

Harvesting of tissue culture cells could be challenging. Here is an easy protocol for efficient harvesting of cells for flow cytometry analysis.

The post Protocol: Preparation of Cells for Flow Cytometry first appeared on Bio-Radiations.

Cell Imaging Simplified – the ZOE™ Fluorescent Cell Imager

Bioradiations Staff — Wed, 20 May 2015 22:41:58 +0000

The ZOE fluorescent cell imager features an integrated digital camera and touch-screen interface that makes brightfield and multichannel fluorescence microscopy simple, fast, and intuitive. An easy-to-use alternative to the traditional inverted microscope, ZOE eliminates the complexities of multichannel fluorescence imaging by combining the ease of use of a personal tablet and the power of an inverted microscope.

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Introducing the ZOE™ Fluorescent Cell Imager for Routine Cell Culture and Fluorescent Imaging Applications

Bioradiations Staff — Fri, 15 May 2015 22:06:25 +0000

Using traditional fluorescence microscopes for everyday cell imaging can be frustrating, with a lot of time spent in the darkroom mastering a highly specialized piece of equipment, all to accomplish relatively simple tasks. The ZOE Fluorescent Cell Imager eliminates the complexities of cell imaging by combining the ease of use of a personal tablet with the power of an inverted microscope. Check it out.

The post Introducing the ZOE™ Fluorescent Cell Imager for Routine Cell Culture and Fluorescent Imaging Applications first appeared on Bio-Radiations.

PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells – A Fast Approach to Staining Nuclei

Bioradiations Staff — Mon, 12 Jan 2015 20:00:47 +0000

PureBlu Hoechst 33342 Nuclear Staining Dye is the optimal choice for staining the nuclei of live cells for ultraviolet imaging. This short video goes through the steps involved in staining live cells with PureBlu Hoechst 33342 Nuclear Staining Dye.

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PureBlu™ DAPI Nuclear Staining Dye for Fixed Cells – A Fast Approach to Staining Nuclei

Bioradiations Staff — Mon, 12 Jan 2015 20:00:43 +0000

PureBlu DAPI Nuclear Staining Dye is the optimal choice for staining the nuclei of fixed cells for ultraviolet imaging. This short video goes through the steps involved in staining live cells with PureBlu DAPI Nuclear Staining Dye.

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Investigating Cancer Stem Cells with the S3™ Cell Sorter

Bioradiations Staff — Tue, 09 Dec 2014 23:54:02 +0000

The Cancer Stem Cell model proposes that a special group of dormant cells in the cancer population plays a key role in tumor development and growth, as well as regeneration following treatment. Find out how researchers Stacy Blain and Danielle Joseph are using the S3 Cell Sorter in studying potential cancer stem cells for multiple myeloma.

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Investigating Cancer Stem Cells with the S3™ Cell Sorter

Bioradiations Staff — Tue, 09 Dec 2014 20:00:56 +0000

At SUNY Downstate Medical Center in Brooklyn, New York, researchers Stacy Blain and Danielle Joseph are trying to find out how the Cancer Stem Cell model might apply to multiple myeloma. See how they use Bio-Rad’s S3 Cell Sorter to isolate a small population of cells that might play an important role in this disease.

The post Investigating Cancer Stem Cells with the S3™ Cell Sorter first appeared on Bio-Radiations.

Bio-Rad’s S3 Cell Sorter Development: Redesigning Cell Sorting Technology

Bioradiations Staff — Wed, 08 Oct 2014 00:54:43 +0000

Take a look into the development of Bio-Rad’s S3 Cell Sorter, and see the fresh approach to cell sorting technology that resulted in an innovative new design.

The post Bio-Rad’s S3 Cell Sorter Development: Redesigning Cell Sorting Technology first appeared on Bio-Radiations.

ZOE™ Fluorescent Cell Imager

Bioradiations Staff — Sat, 02 Aug 2014 19:14:48 +0000

Launch the ZOE Interactive Web App to try the intuitive ZOE user interface, manipulate images, view features and specifications in depth, and more.

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Developing Bio-Rad’s S3™ Cell Sorter: The Great Cell Sorting Redesign

Bioradiations Staff — Thu, 15 May 2014 04:53:34 +0000

Cell sorting technology is not new. However, sometimes taking a new look at a mature technology brings unexpected results. See how we reimagined drop delay calculation, rethought software and automation, and more during the design and development of Bio-Rad’s S3 Cell Sorter.

The post Developing Bio-Rad’s S3™ Cell Sorter: The Great Cell Sorting Redesign first appeared on Bio-Radiations.

DIY Cell Sorting with Bio-Rad’s S3™ Cell Sorter

Bioradiations Staff — Tue, 08 Apr 2014 17:13:56 +0000

Until recently, cell sorting has been a complex endeavor requiring specialized facilities and trained technicians. This infographic reveals how Bio-Rad’s S3 cell sorter makes this technique available to individual, non-expert researchers in 3 easy steps.

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TC20™ and TC10™ Cell Counters – Cleaning the Print Heads for Clear Printed Results

Bioradiations Staff — Mon, 07 Apr 2014 20:11:39 +0000

Most problems with misaligned printouts on the printer supplied with the TC20 and TC10 cell counters can be solved by cleaning the print heads. In this short video, find out the fast, easy, and effective way to clean these print heads so you can get back to your research!

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A New Breed of Cell Sorters: Bio-Rad’s S3™ Cell Sorter Appeals to the Growing Cell Biology Community

Bioradiations Staff — Tue, 11 Mar 2014 19:00:00 +0000

The S3 cell sorter is Bio-Rad’s new addition to the world of flow cytometry. Take a look at how this instrument breaks the mold for cell sorters, brings relief to investigators and core lab managers alike, and opens up exciting new research possibilities.

The post A New Breed of Cell Sorters: Bio-Rad’s S3™ Cell Sorter Appeals to the Growing Cell Biology Community first appeared on Bio-Radiations.

New and Improved Microplate Manager® Software for Microplate Data Acquisition and Analysis

Bioradiations Staff — Wed, 27 Mar 2013 17:16:23 +0000

Bio-Rad’s microplate readers function at their full potential when used with Microplate Manager Software. This updated version of the software features two new read modes, compatibility with the latest operating systems, and more.

The post New and Improved Microplate Manager^® Software for Microplate Data Acquisition and Analysis first appeared on Bio-Radiations.

Bio-Rad’s New S3™ Cell Sorter Automates and Simplifies Cell Sorting

Bioradiations Staff — Mon, 29 Oct 2012 19:11:32 +0000

Cell sorting is a complex and expensive process, with complicated steps requiring training. Bio-Rad’s new S3™ cell sorter is compact, easy to use, automated, affordable, and provides exceptional sort purity and yield results.

The post Bio-Rad’s New S3™ Cell Sorter Automates and Simplifies Cell Sorting first appeared on Bio-Radiations.

New TC20™ Automated Cell Counter Facilitates Quick Counting with Precision

Bioradiations Staff — Wed, 05 Sep 2012 20:10:10 +0000

Bio-Rad’s new TC20 automated cell counter is compatible with broad range of cell sizes and types such as cell lines, primary cells (from tissue or blood), and stem cells.

The post New TC20™ Automated Cell Counter Facilitates Quick Counting with Precision first appeared on Bio-Radiations.

QX100™ Droplet Digital™ ddPCR™ System at Sangamo BioSciences

Bioradiations Staff — Fri, 13 Jul 2012 22:36:02 +0000

Researchers at Sangamo BioSciences, Inc. explain the benefits of using third generation PCR as it applies to their research in the field of personalized medicine. Watch to see the latest generation of quantitative PCR in action.

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The QX100™ Droplet Digital™ PCR System Workflow

Bioradiations Staff — Mon, 16 Apr 2012 19:00:10 +0000

Watch the step-by-step workflow for absolute quantification of DNA molecules using the third generation droplet digital PCR technology.

The post The QX100™ Droplet Digital™ PCR System Workflow first appeared on Bio-Radiations.

Accurate Counting of Bio-Plex® Magnetic or Polystyrene Beads Using the TC10™ Automated Cell Counter

Bioradiations Staff — Wed, 28 Sep 2011 20:15:04 +0000

This report demonstrates that the TC10 automated cell counter is a suitable alternative to the Coulter Counter and hemocytometer for the preparation of custom Bio-Plex assays.

The post Accurate Counting of Bio-Plex^® Magnetic or Polystyrene Beads Using the TC10™ Automated Cell Counter first appeared on Bio-Radiations.

Targeting Suppression of B-Cell Lymphoma Proliferation

Bioradiations Staff — Wed, 15 Jun 2011 19:00:29 +0000

Rossi considers the discovery of RNAi as pivotal to antiviral discovery. "RNAi has become another important, powerful genetic tool we can use to further our antiviral work," he says. Rossi's lab became the first to publish research demonstrating the expression of small interfering RNAs (siRNAs) to inhibit HIV replication in human cells. Various aspects of RNAi and siRNAs continue to play a key role in his research on viral diseases and lymphomas.

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Unlocking the Potential of Stem Cell Research Using the EpiQ™ Chromatin Analysis Kit

Bioradiations Staff — Mon, 13 Jun 2011 19:00:05 +0000

Bedtime stories in Dr David Schaffer's — one of today's pioneers in stem cell research — childhood home were often not standard fairy tales. With both parents in careers as biomedical researchers (his mother in drug development and clinical trials for a major pharmaceutical company and his father, in cardiovascular research and a pharmacology professor) much of the conversation as far back as Schaffer can remember centered on biology and science.

The post Unlocking the Potential of Stem Cell Research Using the EpiQ™ Chromatin Analysis Kit first appeared on Bio-Radiations.

Comparison between the TC10™ Automated Cell Counter and the Lactate Dehydrogenase (LDH) Assay to Assess Cellular Toxicity in vitro

Bioradiations Staff — Thu, 09 Jun 2011 19:00:24 +0000

This tech report presents the results of a comparative study between the TC10 automated cell counter and the classical LDH assay to measure cell viability in suspension cells. Results show that the TC10 cell counter can replace the LDH assay to measure both the viability of cell suspensions and the cytotoxic effects of various compounds.

The post Comparison between the TC10™ Automated Cell Counter and the Lactate Dehydrogenase (LDH) Assay to Assess Cellular Toxicity in vitro first appeared on Bio-Radiations.

Using the TC10™ Automated Cell Counter

Bioradiations Staff — Thu, 09 Jun 2011 19:00:22 +0000

A better, more accurate, easier way to count cells than a traditional hemocytometer — learn how this automated system works.

The post Using the TC10™ Automated Cell Counter first appeared on Bio-Radiations.