ArticlesCell BiologyElectrophoresis/Western Blotting

In-Cell Western: A Versatile Tool for Quantitative Analysis of Protein Expression In-Situ

Protein expression analysis is essential in cell biology research, as it provides valuable insights into the molecular mechanisms that underlie cellular functions. Traditional methods for protein analysis, such as western blotting and ELISA, are useful for studying protein expression in cell lysates. However, these methods require labor-intensive sample preparation and do not provide information on the spatial distribution of proteins within intact cells. In-Cell Western is a powerful technique that enables quantitative and qualitative in situ protein expression analysis.
READ MORE →
Electrophoresis/Western Blotting VideoTips and Techniques VideoVideos

Quick Tips for Western Blotting

Learn how to improve your western blotting results with our Quick Tips video series. Watch these 1–2 minute videos see how to perfect each step of the western blotting process.
READ MORE →
Crossword puzzle header image
ArticlesBreak RoomElectrophoresis/Western Blotting

Across the Blot: A Western Blotting Crossword

Do you think you know western blotting? Test your knowledge with a crossword puzzle.
READ MORE →
ArticlesFeatured StoriesGeneral Interest

Scientists for Life: A Tale of Two Tattoos

Most scientists, no matter where they are at in their career, remember the moments of wonder as they navigated lab work for the first time. Whether it’s a band on a gel, a colony on a plate, or a piece of data, those first tangible results come with a feeling of having accomplished something incredible. More than ten years later, we caught up with two scientists who immortalized that feeling in the form of matching protein ladder tattoos, and discussed how their time as undergraduate researchers led them where they are today.
READ MORE →
ArticlesCell Biology

5 Reasons to Read the Antibody Advice Guide

In the day-to-day pressures of a research environment, it is natural to want to generate important data as quickly as possible. However, there are real benefits in ensuring that the antibody you have picked is the best for your experiment and spending time aliquoting and titrating your antibodies. Bio-Rad recently launched a free Antibody Advice Guide to equip scientists with the knowledge to get the best out of their experiments with antibodies. Read on for five examples of valuable information that you will learn from the guide, helping you generate publication-ready data.
READ MORE →
Electrophoresis/Western Blotting VideoVideos

Choosing Validated Antibodies for Trustworthy Data

The lack of reproducibility in research has been a source of frustration for scientists. Learn why antibody validation is critical for ensuring specificity and what validation methods Bio-Rad is implementing. Our scientists validate the performance of antibodies through CRISPR-Cas9 gene editing by knockout (KO), siRNA knockdown (KD), and immunoprecipitation followed by mass spectrometry (IP-MS).
READ MORE →
Resolved — comic series banner
ArticlesBreak RoomElectrophoresis/Western BlottingProtocols and Tips

Resolved — Comic Series

Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.
READ MORE →
ArticlesElectrophoresis/Western BlottingProduct Highlights

Introducing the GelDoc Go Imaging System

The next iteration of Bio-Rad Gel Documentation Systems has arrived. The GelDoc Go Imaging System gives you a benchtop imaging solution that enables gel documentation in three simple steps. Acquire high-resolution, publication-quality images of nucleic acid gels, protein gels, and colorimetric blots. Image up to four mini gels at once with the large imaging area, and easily set up and control the GelDoc Go Imaging System with Image Lab Touch Software. Read more about the compact, easy-to-use GelDoc Go System.
READ MORE →
ArticlesElectrophoresis/Western Blotting

Stain-Free Western Blotting: Faster Results, Better Data

Western blotting is a well-accepted technique which has a place in every molecular biologist’s toolkit. However, the traditional process is lengthy and filled with manual steps, which can result in variability. Stain-Free technology can shorten your workflow and improve your data. Read our top three reasons to incorporate Stain-Free technology in your western blotting workflow.
READ MORE →
Western Blot University Webinar header image
Electrophoresis/Western BlottingGeneral InterestPast WebinarsWebinars

Western Blot University

Join us for an in-depth dive into western blotting. Whether you are new to western blotting or an experienced user looking for tips, this five-part webinar series has something for you. Slides and downloadable resources will be available for each webinar. Complete all five classes to receive a certificate of completion and a colorful Western Blotting University poster.
READ MORE →