Bench PartnersBench Partners Video SeriesChromatography VideoTips and Techniques VideoVideos

Removing Air Bubbles from the NGC™ Chromatography System

This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles.

Previous post

Using the Air Sensor on the NGC™ Chromatography System

Next post

Synthesis and Purification of Recombinant Proteins — A Success Story