Chromatography VideoVideos

Coupling Automated Chromatography with Faster Confirmation of Purity and Yield

We’ve combined the chromatography process with purity and yield assessment using stain-free gels to shorten and optimize your protein purification workflow. See how it’s done.
READ MORE →
Bench PartnersBench Partners Video SeriesChromatography VideoTips and Techniques VideoVideos

Attaching a Dynaloop to the NGC™ Chromatography System

This video describes the three main steps in attaching a DynaLoop Sample Loop to the NGC™ Chromatography System to enable the injection of larger sample volumes: connecting the DynaLoop Sample Loop to the NGC Chromatography System, priming the DynaLoop Sample Loop, and creating a method in ChromLab™ Software.
READ MORE →
Bench PartnersBench Partners Video SeriesChromatography VideoTips and Techniques VideoVideos

Calibrating the pH Valve on the NGC™ Chromatography System

The NGC pH valve provides real-time, in-line monitoring of buffer pH. In this video, you’ll learn how to connect the pH valve to the NGC System, add it to the flow path, and calibrate the valve using buffers at two different pH values. Features of the pH valve include accessible calibration ports and easy bypass of the probe when the NGC System is being cleaned.
READ MORE →
Bench PartnersBench Partners Video SeriesChromatography VideoTips and Techniques VideoVideos

Removing Air Bubbles from the NGC™ Chromatography System

This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles.
READ MORE →
Bench PartnersBench Partners Video SeriesChromatography VideoTips and Techniques VideoVideos

Using the Air Sensor on the NGC™ Chromatography System

This video describes the functions of the Air Sensor Module and how to connect it to the NGC Chromatography System. The NGC Air Sensor senses the end of sample or buffer and prevents the introduction of air into the system, which can damage columns and/or incur the loss of precious samples. Both small and large air sensors are available to fit the tubing being used. Steps for connecting the Air Sensor Module and associated air sensors are described including how to add the air sensors into the system flow path using ChromLab™ Software and the differences in using the air sensors in manual vs. method modes.
READ MORE →
ArticlesChromatographyChromatography VideoProduct HighlightsVideos

How Do You Automate a Multicolumn Protein Purification?

Multicolumn purifications are tedious and the manual operations during this process introduce variabilities, affecting the success of purification and reproducibility of results. This whiteboard animation explains how an automated multi-dimensional chromatography method can make this an easier process with very high success rates.
READ MORE →
ArticlesChromatographyChromatography VideoVideos

How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution?

The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.
READ MORE →
Chromatography VideoProduct Demonstration VideoVideos

High-Processivity Sso7d Fusion Polymerase Improves qPCR Results

Processivity is the rate at which nucleotides are added to extend PCR products. High processivity results in a greater number of nucleotides incorporated in each polymerase binding event.
READ MORE →
Bench PartnersChromatography VideoLab NotesVideos

Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography

From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don’t have to worry about proteases, we share helpful tips for developing a sample preparation protocol.
READ MORE →
Bench PartnersChromatography VideoLab NotesVideos

Five Tips for Picking the Right Cell Disruption Method for Protein Analysis

Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.
READ MORE →