Articles

Better model systems and faster, more comprehensive screens are only two of the many ways in which CRISPR is changing the pace of discovery. See how CRISPR is transforming research and development by providing us with tools that were once unimaginable.

In 2016, we published a wide variety of general interest articles, customer testimonials, tips, protocols, videos, product highlight articles, and technical and application notes. Here are the ten articles you found the most interesting in 2016.

Digital PCR offers a highly sensitive alternative to conventional qPCR, enabling absolute quantification of nucleic acids and rare allele detection. The tips in this article help you set up and optimize your digital PCR experiments when using droplet partitioning technology.

Immunoprecipitation (IP) experiments and the detection of IP samples by western blotting can be quite challenging at times. The tips in this article help you set up and optimize your overall IP experimenta design.

Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.

Introduce students (and teachers) to the concepts of photosynthesis and cellular respiration in the context of how these processes evolved and how they affect our daily lives.

Part III of our cell health series describes the three main autophagy mechanisms and reviews the various methods for assessing macroautophagy and autophagic flux in cells.

A well-designed multidimensional (Multi-D) chromatography process, which utilizes optimized individual column methods, can help automate multistep protein purification processes. This can help you save time and increase productivity. See how you can use Multi-D chromatography to expedite the purification of polyhistidine-tagged proteins when using a typical capture, intermediate, and polish purification workflow.

CRISPR has revolutionized gene editing but is not the only way to edit a gene. We compare the pros and cons of four main gene editing techniques – meganucleases, zinc finger nucleases, TALENs, and CRISPR-Cas9 – discuss the advance of these methods to the clinic for therapeutic applications, and introduce newer technologies that eliminate some of the pitfalls of current techniques.

Monocytes play key roles in mediating innate and adaptive immune responses. Here is an overview of the types, functions, process of recruitment, and the role of monocytes in disease.