Normalization of western blot data is very crucial in quantitating proteins. Normalization of band intensity of proteins of interest with the band intensity of housekeeping proteins (HKP) is routinely done in labs. But more and more journals question the validity of using HKPs in normalization and are demanding more validations and tests. An alternative method is to use total protein normalization (TPN) for normalization. Explore the TPN option and see how you can get reliable western blot data easily.
Cancer is one of the leading causes of death in the world and, due to an aging population, more and more people are diagnosed with cancer every year. Researchers are actively searching for treatment options in the form of potential biomarkers. Long noncoding RNAs (lncRNAs) have piqued the interest of researchers as a relatively new source of therapeutics. Learn more about lncRNAs and see how they can be used in cancer diagnosis and intervention.
It is hoped that the development of biosimilars will lead to more accessible and cost-effective treatment options. Here we explore the current opportunities for biosimilars in rheumatoid arthritis and discuss the opportunities and concerns tied to their use.
Biosimilars are gaining in popularity and estimates predict that the U.S. could save between $40–250 billion in healthcare costs over the next decade by switching from biologics to biosimilars. See how biosimilars have gained in popularity recently, the challenges the U.S. faces in developing them, and what the future holds.
In 2017, Bioradiations published a wide variety of general interest articles, tips, podcasts, and application notes. Revisit the technological advancements and growth in technology that researchers experienced this year. Here are the highlights of what we learned this year.
Explore the most watched Bio-Rad videos in 2017. From understanding CRISPR gene editing to generating better qPCR data to selecting antibodies for fluorescent western blotting, these videos highlight how we continually strive to help our customers do better science.
Stem cells possess the remarkable potential to self-renew and to differentiate into many different types of cells. Due to their unique ability to regenerate, they are being used in regenerative medicine and offer great hope for treating diseases such as diabetes and heart disease. However, since stem cells represent a rare population of cells, they are not always easy to study. See how the simplicity, efficiency, and flexibility of cell sorting is making it possible to isolate pure populations of stem cells for downstream analyses.
Cancer kills eight million people annually worldwide; this number will increase to 13 million by 2020. Lung, breast, colorectal, and prostate cancer are the four most commonly diagnosed cancers. Meaningful developments in immuno-oncology, or cancer immunotherapy, treatments are important now more than ever. Learn about some of the newer immunotherapies, including BiTE Antibodies and vaccines, and the companies developing them.
Apolipoproteins bind to lipids to form lipoproteins, which transport lipids throughout the lymphatic and circulatory systems. In addition to cardiovascular disease, apolipoproteins are now being linked to other diseases such as cancer, autoimmunity, and neurodegeneration. Learn more about the different types of apolipoproteins and see how they serve as biomarkers for these diseases.